Team:Clemson

From 2014.igem.org

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<center><a href="https://2013.igem.org/Team:Lethbridge/project"><image src="https://static.igem.org/mediawiki/2013/0/05/ULeth2013iGEM_Mainpage_PKFig.png" width="600px" height="400px"/></a></center>
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=Welcome to the Official Page of Clemson iGEM!=
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==Our Mission==
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<h2>Project Overview</h2>
 
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<p style="color:black">This year, the Lethbridge iGEM team is working to bring a whole new class of parts to the iGEM community: programmed ribosomal frameshifting elements. To do this, we have been working towards standardizing the PK401 pseudoknot for use within the BioBrick system. These RNA secondary structural elements cause the ribosome to switch between translational frames and give another degree of freedom when engineering genetic circuits.</p><br>
 
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<b style="color:black"><u>WHAT?</u></b>
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The FDA has maintained a zero-tolerance policy for several foodborne pathogens.  For example, a policy of “zero-tolerance” for ''Listeria monocytogenes'' in ready-to-eat foods means that the detection of any ''L. monocytogenes'' in either of two 25 gram samples of a food renders the food adulterated; the infectious dosage of ''E. coli'' O157:H7 has been determined to be 10 cells; the Environmental Protection Agency standard for ''E. coli'' O157:H7 in water is 40 cells per liter.  The current detection methods suffer from one or more of the following limitations: 1) the requirement of pre-enrichment and enrichment to increase the number of target pathogens, e.g., bio-chemical assays and immunoassays, 2) high detection limit, e.g., 10^3 – 10^5 CFU per ml or per gram of sample for immunoassays, 3) inability to distinguish viable from non-viable cells, e.g., PCR-based detection methods, 4) small sample volume capacity, e.g., microfluidic-based biosensors (µl instead of the required ml to liter capacity), 5) tedious detection procedures, and 6) the current high per-assay cost.
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<ul style="color:black"><li>Our project is directed towards standardizing pseudoknots to make a new class of parts available to the synthetic biology community</li></ul>
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The aim of this project is develop a Universal Self-Amplified (USA) Biosensor that addresses the aforementioned disadvantages of current detection methods. This two component system utilizes a universal signal amplification bacterial system and a unique pathogen-specific detection counterpart for a one-step detection of target microorganisms in a scalable volume.
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<b style="color:black"><u>WHY?</u></b>
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<ul style="color:black"><li>As the field of synthetic biology grows, so should its toolset. By introducing a standardized method of implementing programmed ribosomal frameshifts in synthetic gene networks, we could not only enable others to reduce plasmid size and regulate operon expression, but also enable them to come up with new, exciting applications</li></ul>
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<b style="color:black"><u>HOW?</b></u>
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<ul style="color:black"><li>We have brought pseudoknots to the iGEM community by:
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<ul><li>Characterizing their function in a biobrick system</li>
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<li>Designing software that enables others to dual code proteins</li>
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<li>Ensuring that the release of these tools to the wider public does not pose a significant risk to the rest of the world</li></li></ul></ul><br>
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<h2>Sponsors</h2>
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<p><center><image src="https://static.igem.org/mediawiki/2013/0/00/ULeth2013_Sponsors_-_Platinum.png"; width="200px"; height="100px" />&nbsp;&nbsp;&nbsp;&nbsp;
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<image src="https://static.igem.org/mediawiki/2013/1/1e/ULeth2013_Sponsors_-_Silver.png"; width="200px"; height="100px" />&nbsp;&nbsp;&nbsp;&nbsp;
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<image src="https://static.igem.org/mediawiki/2013/4/4c/ULeth2013_Sponsors_-_Bronze.png"; width="300px"; height="100px" /></center></p>&nbsp;&nbsp;&nbsp;&nbsp;
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Revision as of 00:16, 17 October 2014

Welcome to the Official Page of Clemson iGEM!

<image src=”https://static.igem.org/mediawiki/2013/c/c7/TillmanPaw.png”width="600px" height="400px"/></a></center>

Our Mission

The FDA has maintained a zero-tolerance policy for several foodborne pathogens. For example, a policy of “zero-tolerance” for Listeria monocytogenes in ready-to-eat foods means that the detection of any L. monocytogenes in either of two 25 gram samples of a food renders the food adulterated; the infectious dosage of E. coli O157:H7 has been determined to be 10 cells; the Environmental Protection Agency standard for E. coli O157:H7 in water is 40 cells per liter. The current detection methods suffer from one or more of the following limitations: 1) the requirement of pre-enrichment and enrichment to increase the number of target pathogens, e.g., bio-chemical assays and immunoassays, 2) high detection limit, e.g., 10^3 – 10^5 CFU per ml or per gram of sample for immunoassays, 3) inability to distinguish viable from non-viable cells, e.g., PCR-based detection methods, 4) small sample volume capacity, e.g., microfluidic-based biosensors (µl instead of the required ml to liter capacity), 5) tedious detection procedures, and 6) the current high per-assay cost.

The aim of this project is develop a Universal Self-Amplified (USA) Biosensor that addresses the aforementioned disadvantages of current detection methods. This two component system utilizes a universal signal amplification bacterial system and a unique pathogen-specific detection counterpart for a one-step detection of target microorganisms in a scalable volume.

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