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Team:Cambridge-JIC/Guide/Transformation/Outline
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| - | Agrobacterium mediated transformation is extensively used in many plant and fungal chassis. For Marchantia, two protocols have been developed to optimise speed and efficiency of transformation | + | Agrobacterium mediated transformation is extensively used in many plant and fungal chassis. For Marchantia, two main protocols have been developed to optimise speed and efficiency of transformation: |
| - | + | The | |
| - | The [[Team:Cambridge-JIC/Guide/Transformation/Cocultivation| cocultivation]] | + | -[[Team:Cambridge-JIC/Guide/Transformation/Cocultivation| cocultivation]] method |
| + | -[[Team:Cambridge-JIC/Guide/Transformation/AgarTrap| agar trap]] methods | ||
==Timing== | ==Timing== | ||
Revision as of 23:44, 16 October 2014
Outline
Agrobacterium mediated transformation is extensively used in many plant and fungal chassis. For Marchantia, two main protocols have been developed to optimise speed and efficiency of transformation: The - cocultivation method - agar trap methods
Timing
The cocultivation method requires spores to be (at least) 5 days old prior to cocultivation with agrobacterium, and the agar trap 3 days old. Agrobacteria takes 3 days to grow after transformation.
For each method, your timing will be optimal if you prepare spores and transform agrobacteria on day 1.
