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Team:Cambridge-JIC/Guide/Transformation/Outline
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Agrobacterium mediated transformation is extensively used in many plant and fungal chassis. For Marchantia, two protocols have been developed to optimise speed and efficiency of transformation. | Agrobacterium mediated transformation is extensively used in many plant and fungal chassis. For Marchantia, two protocols have been developed to optimise speed and efficiency of transformation. | ||
| - | The [[cocultivation]] and [[agar trap | + | The [[Team:Cambridge-JIC/Guide/Transformation/Cocultivation| cocultivation]] and [[Team:Cambridge-JIC/Guide/Transformation/AgarTrap| agar trap]] methods. |
| - | The cocultivation requires spores to be 5 days old prior to cocultivation with agrobacterium, and the agar trap 3 days old. | + | ==Timing== |
| + | The cocultivation method requires spores to be (at least) 5 days old prior to cocultivation with agrobacterium, and the agar trap 3 days old. Agrobacteria takes 3 days to grow after [[Team:Cambridge-JIC/Guide/Transformation/TransformingAgro | transformation]]. | ||
| + | '''For each method, your timing will be optimal if you [[Team:Cambridge-JIC/Guide/Care/PreparingSpores| prepare spores]] and [[Team:Cambridge-JIC/Guide/Transformation/TransformingAgro| transform agrobacteria]] on day 1. | ||
| + | ''' | ||
Revision as of 23:43, 16 October 2014
Outline
Agrobacterium mediated transformation is extensively used in many plant and fungal chassis. For Marchantia, two protocols have been developed to optimise speed and efficiency of transformation.
The cocultivation and agar trap methods.
Timing
The cocultivation method requires spores to be (at least) 5 days old prior to cocultivation with agrobacterium, and the agar trap 3 days old. Agrobacteria takes 3 days to grow after transformation. For each method, your timing will be optimal if you prepare spores and transform agrobacteria on day 1.
