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Team:UIUC Illinois/Modeling
From 2014.igem.org
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</tr> | </tr> | ||
</table> | </table> | ||
| + | <center><b>Fig1. Caffeine Demethylation Pathway</b>.</center> | ||
<p><img src="https://static.igem.org/mediawiki/2014/c/cb/Demethylation.PNG", width="98%"/></p> | <p><img src="https://static.igem.org/mediawiki/2014/c/cb/Demethylation.PNG", width="98%"/></p> | ||
| + | <center><b>Fig2. Caffeine Dehydrogenase Pathway</b>.</center> | ||
<p><img src="https://static.igem.org/mediawiki/2014/8/87/Cbb1.PNG", width="98%"/></p> | <p><img src="https://static.igem.org/mediawiki/2014/8/87/Cbb1.PNG", width="98%"/></p> | ||
<div style="clear: both;"></div> | <div style="clear: both;"></div> | ||
Revision as of 23:29, 16 October 2014
Mathematical Modeling of Caffeine Degradation Pathway
To predict the result of bioreactor, we used mathematica to solve differential using Michaelis-Menton equation. The strength of utilizing mathematica rather than matlab was that it allowed us to set up the value of several constants as varying rather than setting it as invariant. Kcat & Km values were obtained through research papers by Swati & Sathyanarayana (2006), and Ryan M.Summers (2010).
This is caffeine demehtlyation pathway by demethlyase. It goes from Caffeine to theobromine to 7-methylxanthine to xanthine. The other pathway is caffeine dehydrogenase. It goes from Caffeine to Trimethyl Uric acid.
First equation corresponds to caffeine concentration at different time. Second corresponds to the concentration of next product at different time.
Parameters
| Name | Description |
|---|---|
| Vm | Maximum rate of system |
| Kcat | Maximum number of substrate molecules converted into products |
| Km | Substrate concentration where the reaction rate is half of maximum (depend on both enzyme and substrate) |
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