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Team:UFAM Brazil/7-9-2014
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| - | The transformation with pUC72 using approximately 1. | + | The transformation with pUC72 using approximately 1.000 ng/ul and 1 ng/ul worked!! But our transformation with the DNA diluted from the Kit Parts 2013 (BBa_E0840) didn’t grow any transforming cells!!! =( |
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| - | <p> Today we decided to work on the transformation once again with <i>Escherichia coli</i> DH5α RR1 using Transformation Efficiency Kit as a control and | + | <p> Today we decided to work on the transformation once again with <i>Escherichia coli</i> DH5α RR1 using Transformation Efficiency Kit as a control and DNA diluted from DNAa Parts Kit 2013 (BBa_E0840), using iGEM’s standard transformation protocol. We have to be very careful!!!!</p> |
| - | <p> We had a big meeting with | + | <p> We also had a big meeting with all our team members.</p> |
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| - | <td class="back" ><a href="https://2014.igem.org/Team:UFAM_Brazil/7-8-2014"> | + | <td class="back" ><a href="https://2014.igem.org/Team:UFAM_Brazil/7-8-2014">Back</a></td> |
| - | <td class="next" ><a href="https://2014.igem.org/Team:UFAM_Brazil/7-10-2014">Next | + | <td class="next" ><a href="https://2014.igem.org/Team:UFAM_Brazil/7-10-2014">Next</a></td> |
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Latest revision as of 23:18, 16 October 2014
07/09/2014 | ||
The transformation with pUC72 using approximately 1.000 ng/ul and 1 ng/ul worked!! But our transformation with the DNA diluted from the Kit Parts 2013 (BBa_E0840) didn’t grow any transforming cells!!! =( Today we decided to work on the transformation once again with Escherichia coli DH5α RR1 using Transformation Efficiency Kit as a control and DNA diluted from DNAa Parts Kit 2013 (BBa_E0840), using iGEM’s standard transformation protocol. We have to be very careful!!!! We also had a big meeting with all our team members. | ||
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