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Team:Cambridge-JIC/Guide/Constructs/Terminators
From 2014.igem.org
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| - | Unfortunately, there is a lack of terminators in MP. The only one to find consistent use is [ | + | Unfortunately, there is a lack of terminators in MP. The only one to find consistent use is the [http://parts.igem.org/Part:BBa_K1484215 nopaline synthase terminator]. |
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| + | This is a terminator found on the endogenous A. tumefaciens T-DNA! | ||
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| + | This makes sequencing multi-gene constructs difficult. Slight variations to the sequence can be introduced via PCR, and mutiple repeats of the terminator can be added. | ||
Revision as of 23:15, 16 October 2014
Unfortunately, there is a lack of terminators in MP. The only one to find consistent use is the [http://parts.igem.org/Part:BBa_K1484215 nopaline synthase terminator].
This is a terminator found on the endogenous A. tumefaciens T-DNA!
This makes sequencing multi-gene constructs difficult. Slight variations to the sequence can be introduced via PCR, and mutiple repeats of the terminator can be added.
