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Team:Cambridge-JIC/Guide/Constructs/Reporters
From 2014.igem.org
(Created page with "The following fluorescent proteins have been used with consistent results in MP: [Venus] [MRFP1] [eGFP]. A range of localisation tags can be added to enable to distinguish sourc...") |
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The following fluorescent proteins have been used with consistent results in MP: | The following fluorescent proteins have been used with consistent results in MP: | ||
| - | [Venus] | + | [http://parts.igem.org/Part:BBa_K165005 Venus YFP], MRFP1 and eGFP. |
A range of localisation tags can be added to enable to distinguish sources of expression and to visualise individual cells and nuclei. These include: | A range of localisation tags can be added to enable to distinguish sources of expression and to visualise individual cells and nuclei. These include: | ||
| - | [N7] [LTI]. | + | [http://parts.igem.org/Part:BBa_K1484104 N7] and [http://parts.igem.org/Part:BBa_K1484106 LTI]. |
| - | These can be added | + | These can be added instead of the stop codon for the reporters |
These are clearly visible under fluorescent microscopes and confocal microscopy. Sample images include: | These are clearly visible under fluorescent microscopes and confocal microscopy. Sample images include: | ||
| Line 11: | Line 11: | ||
<img: LTI> | <img: LTI> | ||
| - | + | [[Image:CAM14_eGFP-N7.png|thumb|center|500px|Confocal Microscopy image of eGFP n7. Excitation:488, Detection range: 520-540.]] | |
Fluorescent transformants are visible first from 3-5 days after transformation. | Fluorescent transformants are visible first from 3-5 days after transformation. | ||
| - | + | GUS staining has been successfully used to characterise expression, as seen in in this [http://www.researchgate.net/publication/256611354_Comparison_of_the_MpEF1_and_CaMV35_promoters_for_application_in_Marchantia_polymorpha_overexpression_studies paper] | |
Revision as of 23:06, 16 October 2014
The following fluorescent proteins have been used with consistent results in MP: [http://parts.igem.org/Part:BBa_K165005 Venus YFP], MRFP1 and eGFP.
A range of localisation tags can be added to enable to distinguish sources of expression and to visualise individual cells and nuclei. These include: [http://parts.igem.org/Part:BBa_K1484104 N7] and [http://parts.igem.org/Part:BBa_K1484106 LTI].
These can be added instead of the stop codon for the reporters
These are clearly visible under fluorescent microscopes and confocal microscopy. Sample images include:
<img: LTI>
Fluorescent transformants are visible first from 3-5 days after transformation.
GUS staining has been successfully used to characterise expression, as seen in in this [http://www.researchgate.net/publication/256611354_Comparison_of_the_MpEF1_and_CaMV35_promoters_for_application_in_Marchantia_polymorpha_overexpression_studies paper]
