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Team:Evry/Interlab Study/09-14-2014
From 2014.igem.org
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pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h at 37°C. | pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h at 37°C. | ||
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| + | <i>PSB1C3+E0240+J23114</i>, <i>PSB1C3+E0240+J23115</i> and <i>PSB1C3+E0240+J23116</i>: | ||
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| + | <ul> | ||
| + | <li> PCR colony of 5 colonies for each construction following protocol table 1 and 2. | ||
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| + | <div class="center"> | ||
| + | <div class="thumb tnone"> | ||
| + | <div class="thumbinner" style="width:502px;"> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/75/114gel.jpg" class="image"> | ||
| + | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/7/75/114gel.jpg" width="450px;" class="thumbimage"/> | ||
| + | </a> | ||
| + | <div class="thumbcaption"> | ||
| + | <div class="magnify"> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/75/114gel.jpg" class="internal" title="Enlarge"> | ||
| + | <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | ||
| + | </a> | ||
| + | </div> | ||
| + | <center>GEL 1: 1% agarose gel. PCR product of 6 colonies of the PSB1C3+E0240+J23114 construction. </center> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
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| + | <br> | ||
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| + | <div class="center"> | ||
| + | <div class="thumb tnone"> | ||
| + | <div class="thumbinner" style="width:502px;"> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/4/45/115-116.jpg" class="image"> | ||
| + | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/4/45/115-116.jpg" width="450px;" class="thumbimage"/> | ||
| + | </a> | ||
| + | <div class="thumbcaption"> | ||
| + | <div class="magnify"> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/4/45/115-116.jpg" class="internal" title="Enlarge"> | ||
| + | <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | ||
| + | </a> | ||
| + | </div> | ||
| + | <center>GEL 2: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23116 and PSB1C3+E0240+J23115 constructions.</center> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
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| + | </ul> | ||
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<span class="cd-date">Sep 14</span> | <span class="cd-date">Sep 14</span> | ||
Revision as of 22:47, 16 October 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23101:
Measure of the cells' growth and fluorecence on TECAN infiniteM200.
TECAN FAILED
PSB1C3+E0240+J23106:
pre-culture of the colony 3 in LB+Cam (1:1000) according to the result of PCR of 13th September. Incubation 16h at 37°C.
PSB1C3+E0240+J23114, PSB1C3+E0240+J23115 and PSB1C3+E0240+J23116:
- PCR colony of 5 colonies for each construction following protocol table 1 and 2.
GEL 1: 1% agarose gel. PCR product of 6 colonies of the PSB1C3+E0240+J23114 construction.
GEL 2: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23116 and PSB1C3+E0240+J23115 constructions.
