Template:Nevada/Main

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<font size = "10"><b><center>Countdown to the Jamboree!</font></b> </center> <br>
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                          <p>(775) 682-7336</p>
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                            <p>vzepeda@unr.edu</p>
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<div class="team_title_neon_sign">The BAITswitch</div></br>
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<div class="explination_neon_sign">The <u>B</u>ioorthoginal <u>A</u>uxin <u>I</u>nduceable <u>T</u>rigger <u>Switch</u></div>
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<title>University of Nevada iGEM</title>
 
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<body>
<body>
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<h2>Team Nevada - Coming Soon</h2>
 
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<p>Since its <strong>inception in 2003 </strong> at MIT, the iGEM competition has  grown to an international level  bringing  <strong>thousands of international students </strong>together. iGEM
 
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is dedicated to education through competition, to the advancement of synthetic biology, and the development of open community and collaboration.</p>
 
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<h2>Team Nevada's Goal: </h2>
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                  <p> Plant cellular regulation is as much affected by targeted protein degradation as it is by transcriptional regulation. This is in part due to the strict three-step ubiquitin pathway that involves three enzymes, the last of which, the E3 ubiquitin ligase, comes in over a thousand forms. The many possible complexes of E3 ligase and substrate also allow for a spectrum of concentration sensitivities, a characteristic that is vital to hormone signaling both in plants and humans. Auxin, a plant hormone, is involved in nearly every aspect of plant growth and development. Auxin receptors induce polyubiquitylation of a targeted protein once bound to auxin, which can be thought of as a protein-to-protein glue between the target protein and the E3 ligase. Jasmonates, although very similar to auxin, respond more to environmental stress. JA-Ile receptors mediate the degradation of JAZ proteins which are transcriptional repressors. <br>
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Nevada iGEM is utilizing these hormone based protein degradation pathways found in plants to create inducible protein degradation systems in yeast (and eventually mammalian cells). We are using the auxin and jasmonic acid pathways to test how fast we can manipulate degradation of a protein of interest. Our goals are to use the systems bioorthogonally to have zero cross-talk, or only beneficial cross-talk, and to utilize the mechanisms of the auxin pathway to have variable sensitivities and response time.
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        <p>Donate to help us get to the iGEM Jamboree. All donations are tax deductible. <br>
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<a class="awesome" href="https://supportnevada.unr.edu/sslpage.aspx?pid=355"><b><FONT COLOR="#C0C0C0">Click Here to Become a Sponsor</b></font></a> <p>  *Choose iGEM competition in the drop down box. <br> Contact our team coordinator for more information or if you prefer to donate by check or lab donations! Every little bit helps :)
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            </div><!-- /home-section-wrapper -->
-
      </p>
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         </section>
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         <h2>Our Sponsors: </h2>
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         <!-- /home-section -->
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         <p><img src="https://static.igem.org/mediawiki/2014/8/88/Nv_inbre_logo.jpg" width="375" height="95" class="sponsors">
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<img src="https://static.igem.org/mediawiki/2014/f/ff/Unr_biotechnology.jpg" width="497" height="110" class="sponsors"></p>
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</div>
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<div class="connect">
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        <div id="mc_embed_signup">
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<input type="email" value="" name="EMAIL" class="email" id="mce-EMAIL" placeholder="Subscribe to Our Newsletter" required>
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 +
 
 +
 
 +
        <!--
 +
      =========================================
 +
        Arrows Section
 +
        ========================================== -->
 +
    <!-- arrows-section -->
 +
        <section class="arrows-section">
 +
            <a href="#works-section" title="How It Works" class="scrollto">
 +
                <i class="angle-down"></i>
 +
            </a>
 +
            <a href="#feature-section" title="Awesome Features" class="scrollto">
 +
                <i class="angle-up"></i>
 +
            </a>
 +
        </section><!-- /arrows-section -->
 +
 
 +
 
 +
 
 +
 
 +
        <!-- =========================================
 +
        How it Works Section
 +
        ========================================== -->
 +
        <!-- works-section -->
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        <section id="works-section">
 +
 
 +
            <!-- container -->
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            <div class="container">
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                <!-- row -->
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                <div class="row">
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 +
 
 +
                    <!-- col-md-12 -->
 +
                    <div class="col-md-12">
 +
 
 +
                        <!-- section-title -->
 +
                        <div class="section-title_works_section" data-uk-scrollspy="{cls:'uk-animation-slide-top', delay:500, repeat: true}">
 +
<h1>Overview</h1><style>
 +
 
 +
 
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.lab_2 {
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.lab_2 i {
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    z-index: 50;
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.lab_2 i:nth-child(1) {
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.lab_2 i:nth-child(2) {
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    animation-delay: .7s;
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.lab_2 i:nth-child(3) {
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    animation-delay: 1.5s;
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    animation-name: bubble3;
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.lab_2 i:nth-child(4) {
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    animation-delay: 2.3s;
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    animation-name: bubble4;
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@keyframes bubble3{
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 +
 
 +
@keyframes bubble4{
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}
 +
</style>
 +
 
 +
 
 +
 
 +
  <div class="lab_2">
 +
  <img src="https://static.igem.org/mediawiki/2014/5/50/Nevada_igem_erlemyer_flask_2014.png" alt="lab bottle"/>
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    <i></i>
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    <i></i>
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    <i></i>
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    <i></i>
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</div>                                <style>
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 +
 
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.lab {
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    margin: 0px 0px 0 35%;
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    height: 10px;
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.lab img {
 +
    position: absolute;
 +
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 +
    left: 0;
 +
    z-index: 40;
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}
 +
 
 +
.lab i {
 +
    z-index: 50;
 +
    position: absolute;
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    width: 8px;
 +
    border-radius: 16px;
 +
    opacity: 0;
 +
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 +
 
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.lab i:nth-child(1) {
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    animation-name: bubble1;
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}
 +
 
 +
.lab i:nth-child(2) {
 +
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    animation-name: bubble2;
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 +
 
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 +
    animation-delay: 1.5s;
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    animation-name: bubble1;
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 +
 
 +
.lab i:nth-child(4) {
 +
    animation-delay: 2.3s;
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    animation-name: bubble2;
 +
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 +
 
 +
@keyframes bubble1{
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 +
 
 +
@keyframes bubble2{
 +
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 +
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 +
    100%{bottom:73px; left:13px; transform:scale(5); opacity:0; background:#61d3ff;}
 +
}
 +
</style>
 +
 
 +
  <div class="lab">
 +
  <img src="https://static.igem.org/mediawiki/2014/5/50/Nevada_igem_erlemyer_flask_2014.png" alt="lab bottle"/>
 +
    <i></i>
 +
    <i></i>
 +
    <i></i>
 +
    <i></i>
 +
</div>
 +
                        </div><!-- /section-title -->
 +
 
 +
                        <!-- section-desc -->
 +
                        <div class="section-desc" data-uk-scrollspy="{cls:'uk-animation-fade', delay:700, repeat: true}">
 +
                            <p>This summer, the iGEM team of Nevada came up with a new strategy for rapid repsonse protein degradation in Yeast cells through our <u>B</u>ioorthoginal <u>A</u>uxin <u>I</u>nduceable <u>T</u>rigger <u>Switch</u> (or BAITSwitch). With usual protein degredation being a very slow process which can take several hours, or even days. And the perfect system being similar to that of a light switch; where a response is seen immediately after a trigger is toggled.
 +
                            This year we attempted to select a specifically labeled protein for immediate degradation via the Bioorthoginal auxin-inducible-degron (AID) System (AUTHOR GOES HERE). This bioorthogonal system has been published and used by previous iGEM teams, but has never been combined with its parallel system, jasmonic acid, in non-plant cells. However, beforew we could start our journey we needed to understand some very unique characteristics about Protein Degredation. </p>
 +
                        </div><!-- /section-desc -->
 +
 
 +
                    </div><!-- /col-md-12 -->
 +
 
 +
 
 +
                    <!-- col-md-12 -->
 +
                    <div class="col-md-12">
 +
 
 +
 
 +
                        <!-- WorksTabs -->
 +
                        <div id="WorksTabs">
 +
 
 +
                            <!-- Tabs List -->
 +
                            <ul>
 +
                                <li><a href="#install" title="Understanding the Perfect Model">Understanding the Perfect Model</a></li>
 +
                                <li><a href="#login" title="Typical Protein Degradation Rates">Typical Protein Degradation Rates</a></li>
 +
                                <li><a href="#share" title ="How Protein Degradation Works">How Protein Degradation Works</a></li>
 +
                                <li><a href="#friend" title="The BAITSwitch">The BAITSwitch</a></li>
 +
 
 +
                            </ul><!-- /Tabs List -->
 +
 
 +
 
 +
                            <!-- install -->
 +
                            <div id="install">
 +
 
 +
 
 +
                                <!-- col-md-6 no-pad-left -->
 +
                              <div class="col-md-6 no-pad-left">
 +
 
 +
 
 +
                                    <!-- section-short-title -->
 +
                                    <div class="section-short-title">
 +
                                        <h1>How a Lightbulb Works</h1>
 +
                                    </div><!-- /section-short-title -->
 +
 
 +
 
 +
                                    <!-- section-short-desc -->
 +
                                    <div class="section-short-desc">
 +
                                        <p>At the begining of our journey, we were told how a light switch is the most perfect example of instantinious rate changes.</p>
 +
                                    </div><!-- /section-short-desc -->
 +
 
 +
 
 +
                                    <!-- feature-box-style-2 -->
 +
                                    <div class="feature-box-style-2">
 +
 
 +
                                        <!-- feature-icon -->
 +
                                        <div class="feature-icon">
 +
                                            <i class="bullseye"></i>
 +
                                        </div><!-- /feature-icon -->
 +
 
 +
                                      <!-- feature-title -->
 +
                                        <div class="feature-title">
 +
                                            <h4>Understanding the Perfect Model</h4>
 +
                                        </div><!-- /feature-title -->
 +
 
 +
                                        <!-- feature-desc -->
 +
                                        <div class="feature-desc">
 +
                                            <p>As seen in the graph to the right, a lightswitch is the perfect model from the fact that there is an imediate response (creation of light) after a trigger is activated (ie. flicking on the light switch). With 0 being representation of no light in a room and 1 being the room filled with light over the time span of a couple seconds. </p>
 +
                                        </div><!-- /feature-desc -->
 +
 
 +
                                    </div><!-- /feature-box-style-2 -->
 +
 
 +
 
 +
 +
 
 +
                                   
 +
 
 +
 
 +
                                </div><!-- /col-md-6 no-pad-left -->
 +
 
 +
 
 +
                                <!-- col-md-6 no-pad-right -->
 +
                                <div class="col-md-6 no-pad-right">
 +
 
 +
 
 +
                                        <div class="col-md-6 no-pad-right">
 +
                                        <div class="description_graph_1">
 +
<h1>Turning on a Light</h1>
 +
</div>
 +
<div class="graph__wrapper_graph_1">
 +
  <div class="coordinates_graph_1">
 +
  <span></span>
 +
  <span></span>
 +
  </div>
 +
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 +
      <defs>
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              <stop stop-color="#58407a
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          <path d="M2.10546875,95.75 L40.5546875,95.75 L55.2109375,95.75 L65.2148437,95.75 L96.1835937,95.75 L131.023438,95.75 L142.15625,95.75 L143.15625,.1953125 L211.007812,.1953125 L234.320312,.1953125 L234.667969,.1953125 L244.019531,.1953125 L247.105469,.1953125 L312.695312,.1953125" id="Path-1" stroke="url(#linearGradient-1)" stroke-width="4" sketch:type="MSShapeGroup" class="path_graph_1"></path>
 +
      </g>
 +
  </svg>
 +
</div>
 +
</div>
 +
 
 +
 
 +
                                </div><!-- /col-md-6 no-pad-right -->
 +
 
 +
 
 +
                          </div><!-- /install -->
 +
 
 +
 
 +
 
 +
                            <!-- login -->
 +
                            <div id="login">
 +
 
 +
 
 +
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                                    <div class="section-short-title">
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                                        <h1>Typical Protein Degredation Rates</h1>
 +
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                                    <div class="section-short-desc">
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                                        <p>One of the first things that we needed to understand was how long it usually takes a protein to degrade. As seen in the graph on the left from <a href="https://static.igem.org/mediawiki/2014/4/41/Fates-shifted_is_an_F-box_protein_that_targets_Bicoid_for_degradation_and_regulates_developmental_fate_determination_in_Drosophila_embryos.pdf"><u>Junbo Liu</u></a> and <a href="https://static.igem.org/mediawiki/2014/3/3d/Metabolism_of_Histones_of_Brain_and_Liver.pdf"><u>R. Sakari Piha</u></a>, regualr protein degredation can take from hours to months to eliminate the protein entirely. Hover over the graph or select the individual proteins to see their individual degradation times.</p>
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                                            <h4>BCD Protein and Histone Protein Degradation</h4>
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                                            <p>Maybe put something about the specific proteins that we have in the graphs. </p>
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                                        <h1>How Protein Degradation Works</h1>
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                                        <p>After understanding that a protein's life can be fairly lengthy, we need to learn on how proteins were mechanically actually broken down. </p>
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                                        <div class="feature-title">
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                                            <h4>Ubiquitination</h4>
 +
                                        </div><!-- /feature-title -->
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                                        <!-- feature-desc -->
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                                        <div class="feature-desc">
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                                            <p>There are 3 types of enzymes involved in ubiquitination which make up the ubiquitin-proteasome system (UPS) are called E1, E2, and E3. Together these three enzymes create a "three-step enzymatic cascade, resulting in the covalent transfer of ubiquitin to target proteins" (<a href="https://static.igem.org/mediawiki/2014/f/f5/Plant_Physiol.-2012-Kelley-47-55-nevada-igem-2014.pdf">D. Kelly</a>). </p>
 +
                                            </br>
 +
                                            <p>In the UPS, each enzyme serves a specific function to target the substrate protein for destruction by the 26S proteasome or alters their biochemical properties and subcellular organization in plants. Here, E1 serves as the ubiquitin activating enzyme while containing two isoforms. E2 serves as the ubiquitin-conjugating enzyme and is believed to have at least 40 different derivatives. Then, with E3 serving as the ubiquitin ligase which is predicted to encode for more than a thousand potential E3 ligases. (<a href="https://static.igem.org/mediawiki/2014/8/84/Plant_ubiquitin_ligases_as_signaling_hubs_nevada_igem_2014.pdf">Nitzan Shabek</a>)</p>
 +
                                            </br>
 +
                                            <p>Although, most reasearch shows this system being used in several different species of plant, it also serves the same role in eukaryotic cells as well. (<a href="https://static.igem.org/mediawiki/2014/8/84/Plant_ubiquitin_ligases_as_signaling_hubs_nevada_igem_2014.pdf">Nitzan Shabek</a>)</p>
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                                        <img src="https://static.igem.org/mediawiki/2014/8/80/Ubiquitin_enymes_nevada_igem_2014.gif" alt="Feature Image" width="574" height="733"/>
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                                    <!-- feature-image -->
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                                    <div class="feature-image">
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                                        <img src="images/feature-images/002.png" alt="Feature Image" />
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                                    </div><!-- /feature-image -->
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                                    <div class="section-short-title">
 +
                                        <h1>The BAITSwitch</h1>
 +
                                    </div><!-- /section-short-title -->
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                                    <!-- section-short-desc -->
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                                    <div class="section-short-desc">
 +
                                        <p>Through the method's seen in BLANKS paper, theoretically we can attach a tag (an IAA tag or Jaz tag) onto a protein. Then intoduced the hormore Auxin or Caronite to allow for the imediate degredation of the specified protein. Successfully eliminating all proteins with a specified tag (Seen by BLANK).</p>
 +
                                    </div><!-- /section-short-desc -->
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                                        <!-- feature-title -->
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                                        <div class="feature-title">
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                                            <h4>Description 1</h4>
 +
                                        </div><!-- /feature-title -->
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                                        <!-- feature-desc -->
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                                        <div class="feature-desc">
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                                            <p>Ut egestas orci in quam sollicitudin aliquet.Duis bibendum diam non erat facilaisis tincidunt. Fusce leo neque, lacinia at tempor vitae.</p>
 +
                                        </div><!-- /feature-desc -->
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                                    <!-- feature-box-style-2 -->
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                                    <div class="feature-box-style-2">
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 +
                                        <!-- feature-icon -->
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                                        <div class="feature-icon">
 +
                                            <i class="fa fa-headphones"></i>
 +
                                        </div><!-- /feature-icon -->
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                                        <!-- feature-title -->
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                                        <div class="feature-title">
 +
                                            <h4>Description 2</h4>
 +
                                        </div><!-- /feature-title -->
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 +
                                        <!-- feature-desc -->
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                                        <div class="feature-desc">
 +
                                            <p>Ut egestas orci in quam sollicitudin aliquet.Duis bibendum diam non erat facilaisis tincidunt. Fusce leo neque, lacinia at tempor vitae.</p>
 +
                                        </div><!-- /feature-desc -->
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        <!-- =========================================
 +
        Arrows Section
 +
        ========================================== -->
 +
        <!-- arrows-section -->
 +
        <section class="arrows-section">
 +
            <a href="#portfolio-section" title="Screenshots of App" class="scrollto">
 +
                <i class="angle-down"></i>
 +
            </a>
 +
            <a href="#works-section" title="How It Works" class="scrollto">
 +
                <i class="angle-up"></i>
 +
            </a>
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        </section><!-- /arrows-section -->
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 +
        <!-- =========================================
 +
        Closer Section
 +
        ========================================== -->
 +
        <!-- closer-section -->
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        <section id="closer-section">
 +
 
 +
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                        <!-- section-title -->
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 +
                            <h1>Breakdown of the Auxin-Induciable-Degron (AID) system</h1>
 +
                        </div><!-- /section-title -->
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 +
                        <!-- section-desc -->
 +
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 +
                            <p>Shown below is a breakdown of the BAITswitch and how it is used to degrade proteins of interest.The individual components are shown below.</p>
 +
                        </div><!-- /section-desc -->
 +
                       
 +
                        <img src="https://static.igem.org/mediawiki/2014/d/d6/Proteasome_team_nevada_igem_2014.png" height="175px" width="100px">
 +
<img src="https://static.igem.org/mediawiki/2014/5/5b/Aid_and_iaa_team_nevada_igem_2014.png" height="100px" width="241px">
 +
<img src="https://static.igem.org/mediawiki/2014/1/15/Auxin_team_nevada_igem_2014.png" height="100px" width="241px">
 +
<img src="https://static.igem.org/mediawiki/2014/c/c0/Coi-1_team_nevada_igem_2014.png" height="100px" width="125px">
 +
<img src="https://static.igem.org/mediawiki/2014/9/9b/Coronatine_team_nevada_igem_2014.png" height="200px" width="250px">
 +
<img src="https://static.igem.org/mediawiki/2014/a/a7/Jaz_degron_team_nevada_igem_2014.png" height="100px" width="150px">
 +
<img src="https://static.igem.org/mediawiki/2014/4/4e/Tir-1_team_nevada_igem_2014.png" width="200px" height="100px">
 +
<img src="https://static.igem.org/mediawiki/2014/5/5c/Protein_of_interest_team_nevada_igem_2014.png" height="136.9px" width="150px">
 +
<img src="https://static.igem.org/mediawiki/2014/d/d9/Ubiquitin_team_nevada_igem_2014.png"  height="136.9px" width="150px">
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 +
 
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 +
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 +
                        <!-- magnify-wrapper -->
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                        <div id="magnify-wrapper">
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 +
                            <!-- magnify-container -->
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                            <div id="magnify-container">
 +
                                <img " src="https://static.igem.org/mediawiki/2014/3/3f/Auxin-and-Coronitine-System-Gif-better-quality-smaller-size-nevada-igem-2014.gif" />
 +
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 +
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 +
        <!-- =========================================
 +
        Arrows Section
 +
        ========================================== -->
 +
        <!-- arrows-section -->
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        <section class="arrows-section">
 +
            <a href="#ready-section" title="Ready for iPhone and iPad" class="scrollto">
 +
                <i class="angle-down"></i>
 +
            </a>
 +
            <a href="#closer-section" title="Getting Closer to App" class="scrollto">
 +
                <i class="angle-up"></i>
 +
            </a>
 +
        </section><!-- /arrows-section -->
 +
 
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 +
        <!-- =========================================
 +
        Ready Section
 +
        ========================================== -->
 +
        <!-- ready-section -->
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        <section id="faq-section">
 +
 
 +
            <!-- container -->
 +
            <div class="container">
 +
<iframe src='http://cdn.knightlab.com/libs/timeline/latest/embed/index.html?source=0AtTNcGQ7siOtdDhQN0tINVdjQ1lhUEsybXpvWkRpYlE&font=Merriweather-NewsCycle&maptype=toner&lang=en&height=650' width='100%' height='650' frameborder='0'></iframe>
 +
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 +
 
 +
        <!-- =========================================
 +
        Arrows Section
 +
        ========================================== -->
 +
        <!-- arrows-section -->
 +
        <section class="arrows-section">
 +
            <a href="#team-section" title="Our Team" class="scrollto">
 +
                <i class="angle-down"></i>
 +
            </a>
 +
            <a href="#ready-section" title="Ready for iPhone and iPad" class="scrollto">
 +
                <i class="angle-up"></i>
 +
            </a>
 +
        </section><!-- /arrows-section -->
 +
 
 +
 
 +
 
 +
 
 +
        <!-- =========================================
 +
        Our Team Section
 +
        ========================================== -->
 +
        <!-- team-section -->
 +
        <section id="team-section">
 +
 
 +
            <!-- container -->
 +
            <div class="container">
 +
                <!-- row -->
 +
                <div class="row">
 +
 
 +
 
 +
                    <!-- col-md-12 -->
 +
                    <div class="col-md-12">
 +
 
 +
                        <!-- section-title -->
 +
                        <div class="section-title" data-uk-scrollspy="{cls:'uk-animation-slide-top', delay:500, repeat: true}">
 +
                            <h1>Our Team</h1>
 +
                        </div><!-- /section-title -->
 +
 
 +
                        <!-- section-desc -->
 +
                        <div class="section-desc" data-uk-scrollspy="{cls:'uk-animation-fade', delay:700, repeat: true}">
 +
                            <p>Only hrough the joint effors of all of these students and our wonderful advisor, Team Nevada was able to achieve all of their summer and semester goals.</p>
 +
                        </div><!-- /section-desc -->
 +
 
 +
                    </div><!-- /col-md-12 -->
 +
 
 +
 
 +
 
 +
                    <!-- col-md-12 -->
 +
                    <div class="col-md-12">
 +
 
 +
 
 +
                        <!-- TeamTabs -->
 +
                        <div id="TeamTabs">
 +
 
 +
                            <!-- Tabs List -->
 +
                            <ul>
 +
                                <li>
 +
                                    <a href="#member1" title="Member Name">
 +
                                        <img src="https://static.igem.org/mediawiki/2014/b/b4/Khurram_3_edited_for_website_igem_teamnevada_2014_small.png" alt="Member Thumb" />
 +
                                    </a>
 +
                                </li>
 +
                                <li>
 +
                                    <a href="#member2" title="Member Name">
 +
                                        <img src="https://static.igem.org/mediawiki/2014/c/c4/Janice_2_edited_for_website_igem_teamnevada_2014_small.png" alt="Member Thumb" />
 +
                                    </a>
 +
                                </li>
 +
                                <li>
 +
                                    <a href="#member3" title="Member Name">
 +
                                        <img src="https://static.igem.org/mediawiki/2014/5/55/Matt_2_edited_for_website_igem_teamnevada_2014_small.png" alt="Member Thumb" />
 +
                                    </a>
 +
                                </li>
 +
                                <li>
 +
                                    <a href="#member4" title="Member Name">
 +
                                        <img src="https://static.igem.org/mediawiki/2014/4/47/Josh_1_edited_for_website_igem_teamnevada_2014_small.png" alt="Member Thumb" />
 +
                                    </a>
 +
                                </li>
 +
                                <li>
 +
                                    <a href="#member5" title="Member Name">
 +
                                        <img src="https://static.igem.org/mediawiki/2014/5/5d/Tori_2_edited_for_website_igem_teamnevada_2014_small.png" alt="Member Thumb" />
 +
                                    </a>
 +
                                </li>
 +
                                <li>
 +
                                    <a href="#member6" title="Member Name">
 +
                                        <img src="https://static.igem.org/mediawiki/2014/6/6c/Zoe_1_edited_for_website_igem_teamnevada_2014_small.png" alt="Member Thumb" />
 +
                                    </a>
 +
                                </li>
 +
                            </ul><!-- /Tabs List -->
 +
 
 +
 
 +
                            <!-- member1 -->
 +
                            <div id="member1">
 +
 
 +
                                <!-- col-md-6 no-pad-left -->
 +
                                <div class="col-md-6 no-pad-left">
 +
                                    <!-- member-image -->
 +
                                    <div class="member-image">
 +
                                        <img src="https://static.igem.org/mediawiki/2014/9/9e/Khurram_3_edited_for_website_igem_teamnevada_2014.png" alt="Member Image" />
 +
                                    </div><!-- /member-image -->
 +
                                </div><!-- /col-md-6 no-pad-left -->
 +
 
 +
                                <!-- col-md-6 no-pad-right -->
 +
                                <div class="col-md-6 no-pad-right">
 +
 
 +
                                    <!-- section-short-title -->
 +
                                    <div class="section-short-title">
 +
                                        <h1>Khurram Fahim</h1>
 +
                                    </div><!-- /section-short-title -->
 +
 
 +
                                    <!-- section-short-desc -->
 +
                                    <div class="section-short-desc">
 +
                                        <p>A Dual Major in Biochemistry and Neuroscience</p>
 +
                                    </div><!-- /section-short-desc -->
 +
 
 +
                                    <!-- member-desc -->
 +
                                    <div class="member-desc">
 +
                                        <p>Khurram Fahim is a senior biochemistry and neuroscience major at the University of Nevada, Reno. His future plans include attending medical school and continue pursuing research opportunities. Khurram is working with the auxin pathway, involving the TIR1 E3 ligase and IAA degron tag. He is cloning this plant hormone regulation system into yeast cells with an overall goal for specific protein degradation. His other interests include playing volleyball, snowboarding, staying fit, and being involved in university organizations.</p>
 +
                                    </div><!-- /member-desc -->
 +
 
 +
                               
 +
 
 +
                                </div><!-- /col-md-6 no-pad-right -->
 +
 
 +
                            </div><!-- /member1 -->
 +
 
 +
                            <!-- member2 -->
 +
                            <div id="member2">
 +
 
 +
                                <!-- col-md-6 no-pad-left -->
 +
                                <div class="col-md-6 no-pad-left">
 +
                                    <!-- member-image -->
 +
                                    <div class="member-image">
 +
                                        <img src="https://static.igem.org/mediawiki/2014/8/86/Janice_2_edited_for_website_igem_teamnevada_2014.png" alt="Member Image" />
 +
                                    </div><!-- /member-image -->
 +
                                </div><!-- /col-md-6 no-pad-left -->
 +
 
 +
                                <!-- col-md-6 no-pad-right -->
 +
                                <div class="col-md-6 no-pad-right">
 +
 
 +
                                    <!-- section-short-title -->
 +
                                    <div class="section-short-title">
 +
                                        <h1>Janice Bautista</h1>
 +
                                    </div><!-- /section-short-title -->
 +
 
 +
                                    <!-- section-short-desc -->
 +
                                    <div class="section-short-desc">
 +
                                        <p>A Dual Major in Biochemistry and Molecular Biology and Microbiology and Immunnology</p>
 +
                                    </div><!-- /section-short-desc -->
 +
 
 +
                                    <!-- member-desc -->
 +
                                    <div class="member-desc">
 +
                                        <p>Janice Bautista was born in the Philippines and moved to Las Vegas, Nevada when she was 8. She currently in her fourth year of college at the University of Nevada, Reno majoring in Biochemistry and Molecular Biology and Microbiology and Immunnology. She hopes to work for a biotech or pharmaceutical company upon graduating in May 2015. She loves research and is extremely excited to see all the research being done by all the iGEM teams around the world!</p>
 +
                                    </div><!-- /member-desc -->
 +
 
 +
                                   
 +
 
 +
                                </div><!-- /col-md-6 no-pad-right -->
 +
 
 +
                            </div><!-- /member2 -->
 +
 
 +
                            <!-- member3 -->
 +
                            <div id="member3">
 +
 
 +
                                <!-- col-md-6 no-pad-left -->
 +
                                <div class="col-md-6 no-pad-left">
 +
                                    <!-- member-image -->
 +
                                    <div class="member-image">
 +
                                        <img src="https://static.igem.org/mediawiki/2014/6/64/Matt_2_edited_for_website_igem_teamnevada_2014.png" alt="Member Image" />
 +
                                    </div><!-- /member-image -->
 +
                                </div><!-- /col-md-6 no-pad-left -->
 +
 
 +
                                <!-- col-md-6 no-pad-right -->
 +
                                <div class="col-md-6 no-pad-right">
 +
 
 +
                                    <!-- section-short-title -->
 +
                                    <div class="section-short-title">
 +
                                        <h1>Matthew Hawn</h1>
 +
                                    </div><!-- /section-short-title -->
 +
 
 +
                                    <!-- section-short-desc -->
 +
                                    <div class="section-short-desc">
 +
                                        <p>A Biochemistry Major</p>
 +
                                    </div><!-- /section-short-desc -->
 +
 
 +
                                    <!-- member-desc -->
 +
                                    <div class="member-desc">
 +
                                        <p>Matt Hawn is a Biochemistry Major and is in his fourth year at the University of Nevada, Reno. He is currently interested in attending medical school or working toward his PhD in Biochemistry. On the side, Matt has worked for several companies in website development. His enjoyment of web development has lead him to an interest in photoshop, cinematography, and marketing.</p>
 +
                                    </div><!-- /member-desc -->
 +
 
 +
 
 +
 
 +
                                </div><!-- /col-md-6 no-pad-right -->
 +
 
 +
                            </div><!-- /member3 -->
 +
 
 +
                            <!-- member4 -->
 +
                            <div id="member4">
 +
 
 +
                                <!-- col-md-6 no-pad-left -->
 +
                                <div class="col-md-6 no-pad-left">
 +
                                    <!-- member-image -->
 +
                                    <div class="member-image">
 +
                                        <img src="https://static.igem.org/mediawiki/2014/8/86/Josh_1_edited_for_website_igem_teamnevada_2014.png" alt="Member Image" />
 +
                                    </div><!-- /member-image -->
 +
                                </div><!-- /col-md-6 no-pad-left -->
 +
 
 +
                                <!-- col-md-6 no-pad-right -->
 +
                                <div class="col-md-6 no-pad-right">
 +
 
 +
                                    <!-- section-short-title -->
 +
                                    <div class="section-short-title">
 +
                                        <h1>Josh Beard</h1>
 +
                                    </div><!-- /section-short-title -->
 +
 
 +
                                    <!-- section-short-desc -->
 +
                                    <div class="section-short-desc">
 +
                                        <p>Biochemistry Major</p>
 +
                                    </div><!-- /section-short-desc -->
 +
 
 +
                                    <!-- member-desc -->
 +
                                    <div class="member-desc">
 +
                                        <p>Josh's Bio Goes Here!</p>
 +
                                    </div><!-- /member-desc -->
 +
 
 +
                                    <!-- member-social -->
 +
                                   
 +
 
 +
                                </div><!-- /col-md-6 no-pad-right -->
 +
 
 +
                            </div><!-- /member4 -->
 +
                            <!-- member5 -->
 +
                            <div id="member5">
 +
 
 +
                                <!-- col-md-6 no-pad-left -->
 +
                                <div class="col-md-6 no-pad-left">
 +
                                    <!-- member-image -->
 +
                                    <div class="member-image">
 +
                                        <img src="https://static.igem.org/mediawiki/2014/8/8e/Tori_2_edited_for_website_igem_teamnevada_2014.png" alt="Member Image" />
 +
                                    </div><!-- /member-image -->
 +
                                </div><!-- /col-md-6 no-pad-left -->
 +
 
 +
                                <!-- col-md-6 no-pad-right -->
 +
                                <div class="col-md-6 no-pad-right">
 +
 
 +
                                    <!-- section-short-title -->
 +
                                    <div class="section-short-title">
 +
                                        <h1>Tori Speicher</h1>
 +
                                    </div><!-- /section-short-title -->
 +
 
 +
                                    <!-- section-short-desc -->
 +
                                    <div class="section-short-desc">
 +
                                        <p>biochemistry Major</p>
 +
                                    </div><!-- /section-short-desc -->
 +
 
 +
                                    <!-- member-desc -->
 +
                                    <div class="member-desc">
 +
                                        <p>Troi's bio goes here!</p>
 +
                                    </div><!-- /member-desc -->
 +
 
 +
                           
 +
 
 +
                                </div><!-- /col-md-6 no-pad-right -->
 +
 
 +
                            </div><!-- /member5 -->
 +
<!-- member6 -->
 +
                            <div id="member6">
 +
 
 +
                                <!-- col-md-6 no-pad-left -->
 +
                                <div class="col-md-6 no-pad-left">
 +
                                    <!-- member-image -->
 +
                                    <div class="member-image">
 +
                                        <img src="https://static.igem.org/mediawiki/2014/3/3c/Zoe_1_edited_for_website_igem_teamnevada_2014.png" alt="Member Image" />
 +
                                    </div><!-- /member-image -->
 +
                                </div><!-- /col-md-6 no-pad-left -->
 +
 
 +
                                <!-- col-md-6 no-pad-right -->
 +
                                <div class="col-md-6 no-pad-right">
 +
 
 +
                                    <!-- section-short-title -->
 +
                                    <div class="section-short-title">
 +
                                        <h1>Zoe Meraz</h1>
 +
                                    </div><!-- /section-short-title -->
 +
 
 +
                                    <!-- section-short-desc -->
 +
                                    <div class="section-short-desc">
 +
                                        <p>Biochemistry Major</p>
 +
                                    </div><!-- /section-short-desc -->
 +
 
 +
                                    <!-- member-desc -->
 +
                                    <div class="member-desc">
 +
                                        <p>As a first-generation college student, Zoe did not grow up talking about college but she knew she wanted to pursue science and that pursuit led her to the bounties of biochemistry. It has allowed her to view the world we live in with great detail and admiration and her curiosities push her to experiment and grow every day. Although born in California, moving to Reno and attending UNR has molded Zoe's idea of home into mountains of sage and rabbit brush and a growing sense of community. Her hobbies include gardening, reading, various art forms, and secret plans of becoming a vigilante. She hopes to further her studies through MD/PhD to become a coroner.</p>
 +
                                    </div><!-- /member-desc -->
 +
 
 +
                                   
 +
                                </div><!-- /col-md-6 no-pad-right -->
 +
 
 +
                            </div><!-- /member6 -->
 +
 
 +
                        </div><!-- /TeamTabs -->
 +
 
 +
 
 +
                    </div><!-- /col-md-12 -->
 +
 
 +
 
 +
                </div><!-- /row -->
 +
            </div><!-- /container -->
 +
 
 +
        </section><!-- /team-section -->
 +
 
 +
 
 +
 
 +
 
 +
     
 +
     
 +
        <!-- =========================================
 +
        Arrows Section
 +
        ========================================== -->
 +
        <!-- arrows-section -->
 +
        <section class="arrows-section">
 +
            <a href="#clients-section" title="What Clients Say" class="scrollto">
 +
                <i class="angle-down"></i>
 +
            </a>
 +
            <a href="#faq-section" title="FAQ Section" class="scrollto">
 +
                <i class="angle-up"></i>
 +
            </a>
 +
        </section><!-- /arrows-section -->
 +
 
 +
 
 +
 
 +
 
 +
        <!-- =========================================
 +
        Clients Section
 +
        ========================================== -->
 +
        <!-- clients-section -->
 +
        <section id="clients-section">
 +
 
 +
            <!-- container -->
 +
            <div class="container">
 +
                <!-- row -->
 +
                <div class="row">
 +
 
 +
 
 +
                    <!-- col-md-12 -->
 +
                    <div class="col-md-12">
 +
 
 +
                        <!-- section-title -->
 +
                        <div class="section-title" data-uk-scrollspy="{cls:'uk-animation-slide-top', delay:500, repeat: true}">
 +
                            <h1>Nevada's Contribution to the Parts Registry</h1>
 +
                        </div><!-- /section-title -->
 +
 
 +
                        <!-- section-desc -->
 +
                        <div class="section-desc" data-uk-scrollspy="{cls:'uk-animation-fade', delay:700, repeat: true}">
 +
                            <p>Each part seen below is a part that Team Nevada contributed to the iGEM Parts Registry, along with a full description of what was added/modified. These parts were then used in various experiments to create the BAITswitch.</p>
 +
                        </div><!-- /section-desc -->
 +
 
 +
                    </div><!-- /col-md-12 -->
 +
 
 +
 
 +
 
 +
                    <!-- col-md-12 -->
 +
                    <div class="col-md-12">
 +
 
 +
 
 +
                        <!-- owl-clients -->
 +
                        <div class="owl-clients">
 +
 
 +
 
 +
                            <!-- client-item -->
 +
                            <div class="client-item">
 +
 
 +
                                <!-- clients-content -->
 +
                                <div class="client-content">
 +
 
 +
                                    <!-- client-quote -->
 +
                                    <div class="client-quote">
 +
                                        <p>Jaz1 is a degron tag utilized in the coronatine pathway. The protein of interest is tagged with Jaz1; when coronatine is added to the system, the protein and its tag are linked to the E3 ubiquitin ligase, COI1. Click the picture or the name below to be taken to the parts registry page.</p>
 +
                                    </div><!-- /client-quote -->
 +
 
 +
                                    <!-- client-name -->
 +
                                    <div class="cient-name">
 +
                                      <a href="http://parts.igem.org/Part:BBa_K1347000">  Jaz1 </a>
 +
                                    </div><!-- /client-name -->
 +
 
 +
                                </div><!-- /client-content -->
 +
 
 +
                                <!-- client-image -->
 +
                                <div class="client-image">
 +
                                    <a href="http://parts.igem.org/Part:BBa_K1347000"><img src="https://static.igem.org/mediawiki/2014/a/a7/Jaz_degron_team_nevada_igem_2014.png" alt="Client Image" /></a>
 +
                                </div><!-- /client-image -->
 +
 
 +
                            </div><!-- /client-item -->
 +
 
 +
 
 +
                            <!-- client-item -->
 +
                            <div class="client-item">
 +
 
 +
                                <!-- clients-content -->
 +
                                <div class="client-content">
 +
 
 +
                                    <!-- client-quote -->
 +
                                    <div class="client-quote">
 +
                                        <p>RFP-Jaz1 is a degron tag utilized in the coronatine pathway. The protein of interest is tagged with RFP-Jaz1; when coronatine is added to the system, the protein and its tag are linked to the E3 ubiquitin ligase, COI1. Click the picture or the name below to be taken to the parts registry page.</p>
 +
                                    </div><!-- /client-quote -->
 +
 
 +
                                    <!-- client-name -->
 +
                                    <div class="cient-name">
 +
                                      <a href="http://parts.igem.org/Part:BBa_K1347001"> RFP-Jaz1</a>
 +
                                    </div><!-- /client-name -->
 +
 
 +
                                </div><!-- /client-content -->
 +
 
 +
                                <!-- client-image -->
 +
                                <div class="client-image">
 +
                                    <a href="http://parts.igem.org/Part:BBa_K1347001"><img src="https://static.igem.org/mediawiki/2014/7/7a/Jaz_degron_with_rfp_team_nevada_igem_2014.png" alt="Client Image" /></a>
 +
                                </div><!-- /client-image -->
 +
 
 +
                            </div><!-- /client-item -->
 +
 
 +
 
 +
                            <!-- client-item -->
 +
                            <div class="client-item">
 +
 
 +
                                <!-- clients-content -->
 +
                                <div class="client-content">
 +
 
 +
                                    <!-- client-quote -->
 +
                                    <div class="client-quote">
 +
                                        <p>Jaz6 is a degron tag utilized in the coronatine pathway. The protein of interest is tagged with Jaz6; when coronatine is added to the system, the protein and its tag are linked to the E3 ubiquitin ligase, COI1. Click the picture or the name below to be taken to the parts registry page. </p>
 +
                                    </div><!-- /client-quote -->
 +
 
 +
                                    <!-- client-name -->
 +
                                    <div class="cient-name">
 +
                                        <a href="http://parts.igem.org/Part:BBa_K1347002">Jaz6</a>
 +
                                    </div><!-- /client-name -->
 +
 
 +
                                </div><!-- /client-content -->
 +
 
 +
                                <!-- client-image -->
 +
                                <div class="client-image">
 +
                                  <a href="http://parts.igem.org/Part:BBa_K1347002"> <img src="https://static.igem.org/mediawiki/2014/a/a7/Jaz_degron_team_nevada_igem_2014.png" alt="Client Image"/></a>
 +
                                </div><!-- /client-image -->
 +
 
 +
                            </div><!-- /client-item -->
 +
 
 +
 
 +
                            <!-- client-item -->
 +
                            <div class="client-item">
 +
 
 +
                                <!-- clients-content -->
 +
                                <div class="client-content">
 +
 
 +
                                    <!-- client-quote -->
 +
                                    <div class="client-quote">
 +
                                        <p>COI-1 or Coronatine Insensitive-1 recruits JAZ-1 or JAZ-6 tagged substrates in the presence of Coronatine for degradation by the Proteasome.</p>
 +
                                    </div><!-- /client-quote -->
 +
 
 +
                                    <!-- client-name -->
 +
                                    <div class="cient-name">
 +
                                        <a href="http://parts.igem.org/Part:BBa_K1347003">COI1</a>
 +
                                    </div><!-- /client-name -->
 +
 
 +
                                </div><!-- /client-content -->
 +
 
 +
                                <!-- client-image -->
 +
                                <div class="client-image">
 +
                                  <a href="http://parts.igem.org/Part:BBa_K1347003"> <img src="https://static.igem.org/mediawiki/2014/c/c0/Coi-1_team_nevada_igem_2014.png" alt="Client Image" /> </a>
 +
                                </div><!-- /client-image -->
 +
 
 +
                            </div><!-- /client-item -->
 +
 
 +
 
 +
                         
 +
 
 +
                        </div><!-- /owl-clients -->
 +
 
 +
 
 +
                        <!-- owl-navigation -->
 +
                        <div class="owl-navigation">
 +
                            <a class="clients-prev"><i class='angle-left'></i></a>
 +
                            <a class="clients-next"><i class='angle-right'></i></a>
 +
                        </div><!-- /owl-navigation -->
 +
 
 +
 
 +
                    </div><!-- /col-md-12 -->
 +
 
 +
 
 +
                </div><!-- /row -->
 +
            </div><!-- /container -->
 +
 
 +
        </section><!-- /clients-section -->
 +
       
 +
        <!-- =========================================
 +
        Arrows Section
 +
        ========================================== -->
 +
        <!-- arrows-section -->
 +
        <section class="arrows-section">
 +
            <a href="#recent-section" title="Recent Posts" class="scrollto">
 +
                <i class="angle-down"></i>
 +
            </a>
 +
            <a href="#price-section" title="Select Your Price" class="scrollto">
 +
                <i class="angle-up"></i>
 +
            </a>
 +
        </section><!-- /arrows-section -->
 +
 
 +
 
 +
 
 +
<!-- =========================================
 +
        Recent Section
 +
        ========================================== -->
 +
        <!-- recent-section -->
 +
        <section id="recent-section">
 +
 
 +
            <!-- container -->
 +
            <div class="container">
 +
                <!-- row -->
 +
                <div class="row">
 +
 
 +
 
 +
                    <!-- col-md-12 -->
 +
                    <div class="col-md-12">
 +
 
 +
                        <!-- section-title -->
 +
                        <div class="section-title" data-uk-scrollspy="{cls:'uk-animation-slide-top', delay:500, repeat: true}">
 +
                            <h1>Our Project</h1>
 +
                        </div><!-- /section-title -->
 +
 
 +
                        <!-- section-desc -->
 +
                        <div class="section-desc" data-uk-scrollspy="{cls:'uk-animation-fade', delay:700, repeat: true}">
 +
                            <p>Below is a breakdown of the four major components of our research involving the BAITswitch. The project started with an understanding of previous research and was then broken down into our two systems, the Auxin and Coronatine system. We then move on to discuss our results and our future plans for the BAITswitch. </p>
 +
                        </div><!-- /section-desc -->
 +
 
 +
                    </div><!-- /col-md-12 -->
 +
 
 +
 
 +
                    <!-- col-md-3 -->
 +
                    <div class="col-md-3 col-sm-6">
 +
 
 +
                        <!-- mini-post -->
 +
                        <div class="mini-post" data-uk-scrollspy="{cls:'uk-animation-slide-bottom', delay:500, repeat: true}">
 +
 
 +
                            <!-- post-image -->
 +
                            <div class="post-image">
 +
                                <img src="https://static.igem.org/mediawiki/2014/e/ea/Reaserch_paper_stack_nevada_igem_team_2014.png" alt="Post Image" height="270px" Width="221px"/>
 +
                            </div><!-- /post-image -->
 +
 
 +
                            <!-- post-title -->
 +
                            <div class="post-title">
 +
                                <h2>
 +
                                    <a href="single-post.html" title="Blog Post Title Here">Previous Research and Begining our project</a>
 +
                                </h2>
 +
                            </div><!-- /post-title -->
 +
 
 +
                            <!-- post-date -->
 +
                            <div class="post-date">
 +
                                <h4>Dates to Be Determined</h4>
 +
                            </div><!-- /post-date -->
 +
 
 +
                            <!-- post-content -->
 +
                            <div class="post-content">
 +
                                <p>In this section you can see summaries of the research papers that Team Nevada read to help create the BAITswitch. Additionally, you can see the begining of our research, involving the affects of Auxin and Coronatine on Yeast Cells.</p>
 +
                            </div><!-- /post-content -->
 +
 
 +
                            <!-- post-btn-more -->
 +
                            <div class="post-btn-more">
 +
                                <a href="single-post.html" title="Read More" class="btn btn-lg btn-nesto">Read More</a>
 +
                            </div><!-- /post-btn-more -->
 +
 
 +
                        </div><!-- /mini-post -->
 +
 
 +
                    </div><!-- /col-md-3 -->
 +
 
 +
 
 +
                    <!-- col-md-3 -->
 +
                    <div class="col-md-3 col-sm-6">
 +
 
 +
                        <!-- mini-post -->
 +
                        <div class="mini-post" data-uk-scrollspy="{cls:'uk-animation-slide-bottom', delay:700, repeat: true}">
 +
 
 +
                            <!-- post-image -->
 +
                            <div class="post-image">
 +
                                <img src="https://static.igem.org/mediawiki/2014/6/63/Auxin_hormone_team_nevada_2014.png" height="270px" Width="221px" alt="Post Image" />
 +
                            </div><!-- /post-image -->
 +
 
 +
                            <!-- post-title -->
 +
                            <div class="post-title">
 +
                                <h2>
 +
                                    <a href="single-post.html" title="The Auxin System">The Auxin System</a>
 +
                                </h2>
 +
                            </div><!-- /post-title -->
 +
 
 +
                            <!-- post-date -->
 +
                            <div class="post-date">
 +
                                <h4>Dates to be Determined</h4>
 +
                            </div><!-- /post-date -->
 +
 
 +
                            <!-- post-content -->
 +
                            <div class="post-content">
 +
                                <p>The breakdown of how Team Nevada genetically designed and engineered the Auxin Degreadtion system in the BAITswitch. </p>
 +
                            </div><!-- /post-content -->
 +
 
 +
                            <!-- post-btn-more -->
 +
                            <div class="post-btn-more">
 +
                                <a href="single-post.html" title="Read More" class="btn btn-lg btn-nesto">Read More</a>
 +
                            </div><!-- /post-btn-more -->
 +
 
 +
                        </div><!-- /mini-post -->
 +
 
 +
                    </div><!-- /col-md-3 -->
 +
 
 +
 
 +
                    <!-- col-md-3 -->
 +
                    <div class="col-md-3 col-sm-6">
 +
 
 +
                        <!-- mini-post -->
 +
                        <div class="mini-post" data-uk-scrollspy="{cls:'uk-animation-slide-bottom', delay:900, repeat: true}">
 +
 
 +
                            <!-- post-image -->
 +
                            <div class="post-image">
 +
                                <img src="https://static.igem.org/mediawiki/2014/a/af/Coronatine_hormone_team_nevada_igem_2014.png" height="270px" Width="221px" alt="Post Image" />
 +
                            </div><!-- /post-image -->
 +
 
 +
                            <!-- post-title -->
 +
                            <div class="post-title">
 +
                                <h2>
 +
                                    <a href="single-post.html" title="The Coronatine System">The Coronatine System</a>
 +
                                </h2>
 +
                            </div><!-- /post-title -->
 +
 
 +
                            <!-- post-date -->
 +
                            <div class="post-date">
 +
                                <h4>Dates to be Determined</h4>
 +
                            </div><!-- /post-date -->
 +
 
 +
                            <!-- post-content -->
 +
                            <div class="post-content">
 +
                                <p>The breakdown of how Team Nevada genetically designed and engineered the Coronatine Degreadtion system in the BAITswitch.</p>
 +
                            </div><!-- /post-content -->
 +
 
 +
                            <!-- post-btn-more -->
 +
                            <div class="post-btn-more">
 +
                                <a href="single-post.html" title="Read More" class="btn btn-lg btn-nesto">Read More</a>
 +
                            </div><!-- /post-btn-more -->
 +
 
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         <p> For further information- contact our team coordinator, Veronica Zepeda, vzepeda@unr.edu
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Revision as of 21:41, 16 October 2014

Nevada iGEM 2014

Team Nevada

The BAITswitch

The Bioorthoginal Auxin Induceable Trigger Switch

Overview

lab bottle
lab bottle

This summer, the iGEM team of Nevada came up with a new strategy for rapid repsonse protein degradation in Yeast cells through our Bioorthoginal Auxin Induceable Trigger Switch (or BAITSwitch). With usual protein degredation being a very slow process which can take several hours, or even days. And the perfect system being similar to that of a light switch; where a response is seen immediately after a trigger is toggled. This year we attempted to select a specifically labeled protein for immediate degradation via the Bioorthoginal auxin-inducible-degron (AID) System (AUTHOR GOES HERE). This bioorthogonal system has been published and used by previous iGEM teams, but has never been combined with its parallel system, jasmonic acid, in non-plant cells. However, beforew we could start our journey we needed to understand some very unique characteristics about Protein Degredation.

How a Lightbulb Works

At the begining of our journey, we were told how a light switch is the most perfect example of instantinious rate changes.

Understanding the Perfect Model

As seen in the graph to the right, a lightswitch is the perfect model from the fact that there is an imediate response (creation of light) after a trigger is activated (ie. flicking on the light switch). With 0 being representation of no light in a room and 1 being the room filled with light over the time span of a couple seconds.

Turning on a Light

Typical Protein Degredation Rates

One of the first things that we needed to understand was how long it usually takes a protein to degrade. As seen in the graph on the left from Junbo Liu and R. Sakari Piha, regualr protein degredation can take from hours to months to eliminate the protein entirely. Hover over the graph or select the individual proteins to see their individual degradation times.

BCD Protein and Histone Protein Degradation

Maybe put something about the specific proteins that we have in the graphs.

How Protein Degradation Works

After understanding that a protein's life can be fairly lengthy, we need to learn on how proteins were mechanically actually broken down.

Ubiquitination

There are 3 types of enzymes involved in ubiquitination which make up the ubiquitin-proteasome system (UPS) are called E1, E2, and E3. Together these three enzymes create a "three-step enzymatic cascade, resulting in the covalent transfer of ubiquitin to target proteins" (D. Kelly).


In the UPS, each enzyme serves a specific function to target the substrate protein for destruction by the 26S proteasome or alters their biochemical properties and subcellular organization in plants. Here, E1 serves as the ubiquitin activating enzyme while containing two isoforms. E2 serves as the ubiquitin-conjugating enzyme and is believed to have at least 40 different derivatives. Then, with E3 serving as the ubiquitin ligase which is predicted to encode for more than a thousand potential E3 ligases. (Nitzan Shabek)


Although, most reasearch shows this system being used in several different species of plant, it also serves the same role in eukaryotic cells as well. (Nitzan Shabek)

Feature Image
Feature Image

The BAITSwitch

Through the method's seen in BLANKS paper, theoretically we can attach a tag (an IAA tag or Jaz tag) onto a protein. Then intoduced the hormore Auxin or Caronite to allow for the imediate degredation of the specified protein. Successfully eliminating all proteins with a specified tag (Seen by BLANK).

Description 1

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Description 2

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Breakdown of the Auxin-Induciable-Degron (AID) system

Shown below is a breakdown of the BAITswitch and how it is used to degrade proteins of interest.The individual components are shown below.

Our Team

Only hrough the joint effors of all of these students and our wonderful advisor, Team Nevada was able to achieve all of their summer and semester goals.

Member Image

Khurram Fahim

A Dual Major in Biochemistry and Neuroscience

Khurram Fahim is a senior biochemistry and neuroscience major at the University of Nevada, Reno. His future plans include attending medical school and continue pursuing research opportunities. Khurram is working with the auxin pathway, involving the TIR1 E3 ligase and IAA degron tag. He is cloning this plant hormone regulation system into yeast cells with an overall goal for specific protein degradation. His other interests include playing volleyball, snowboarding, staying fit, and being involved in university organizations.

Member Image

Janice Bautista

A Dual Major in Biochemistry and Molecular Biology and Microbiology and Immunnology

Janice Bautista was born in the Philippines and moved to Las Vegas, Nevada when she was 8. She currently in her fourth year of college at the University of Nevada, Reno majoring in Biochemistry and Molecular Biology and Microbiology and Immunnology. She hopes to work for a biotech or pharmaceutical company upon graduating in May 2015. She loves research and is extremely excited to see all the research being done by all the iGEM teams around the world!

Member Image

Matthew Hawn

A Biochemistry Major

Matt Hawn is a Biochemistry Major and is in his fourth year at the University of Nevada, Reno. He is currently interested in attending medical school or working toward his PhD in Biochemistry. On the side, Matt has worked for several companies in website development. His enjoyment of web development has lead him to an interest in photoshop, cinematography, and marketing.

Member Image

Josh Beard

Biochemistry Major

Josh's Bio Goes Here!

Member Image

Tori Speicher

biochemistry Major

Troi's bio goes here!

Member Image

Zoe Meraz

Biochemistry Major

As a first-generation college student, Zoe did not grow up talking about college but she knew she wanted to pursue science and that pursuit led her to the bounties of biochemistry. It has allowed her to view the world we live in with great detail and admiration and her curiosities push her to experiment and grow every day. Although born in California, moving to Reno and attending UNR has molded Zoe's idea of home into mountains of sage and rabbit brush and a growing sense of community. Her hobbies include gardening, reading, various art forms, and secret plans of becoming a vigilante. She hopes to further her studies through MD/PhD to become a coroner.

Nevada's Contribution to the Parts Registry

Each part seen below is a part that Team Nevada contributed to the iGEM Parts Registry, along with a full description of what was added/modified. These parts were then used in various experiments to create the BAITswitch.

Jaz1 is a degron tag utilized in the coronatine pathway. The protein of interest is tagged with Jaz1; when coronatine is added to the system, the protein and its tag are linked to the E3 ubiquitin ligase, COI1. Click the picture or the name below to be taken to the parts registry page.

Jaz1
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RFP-Jaz1 is a degron tag utilized in the coronatine pathway. The protein of interest is tagged with RFP-Jaz1; when coronatine is added to the system, the protein and its tag are linked to the E3 ubiquitin ligase, COI1. Click the picture or the name below to be taken to the parts registry page.

RFP-Jaz1
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Jaz6 is a degron tag utilized in the coronatine pathway. The protein of interest is tagged with Jaz6; when coronatine is added to the system, the protein and its tag are linked to the E3 ubiquitin ligase, COI1. Click the picture or the name below to be taken to the parts registry page.

Jaz6
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COI-1 or Coronatine Insensitive-1 recruits JAZ-1 or JAZ-6 tagged substrates in the presence of Coronatine for degradation by the Proteasome.

COI1
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Our Project

Below is a breakdown of the four major components of our research involving the BAITswitch. The project started with an understanding of previous research and was then broken down into our two systems, the Auxin and Coronatine system. We then move on to discuss our results and our future plans for the BAITswitch.

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Previous Research and Begining our project

Dates to Be Determined

In this section you can see summaries of the research papers that Team Nevada read to help create the BAITswitch. Additionally, you can see the begining of our research, involving the affects of Auxin and Coronatine on Yeast Cells.

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The Auxin System

Dates to be Determined

The breakdown of how Team Nevada genetically designed and engineered the Auxin Degreadtion system in the BAITswitch.

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The Coronatine System

Dates to be Determined

The breakdown of how Team Nevada genetically designed and engineered the Coronatine Degreadtion system in the BAITswitch.

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Results and Future Application

Dates to Be Determined

In this final section you can see the final results of combining the Auxin and Coronatine System to form the BAITswitch. Furthermore, you can see the future plans that Team Nevada has for the BAITswitch, along with its real world application.

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Stay updated on everything happening with Team Nevada as they journey toward the iGEM Jamboree .

Our Sponsors

Here is a list of all of the people who have helped make Team Nevada's iGEM expierence possible.

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