Team:Arizona State/policypractices

From 2014.igem.org

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<td width="90%" ><p><strong style="font-size: 34px">The Arizona State University iGEM team</strong></p>
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<td width="90%" ><p><strong style="font-size: 34px">Policy</strong>
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<p><strong style="font-size: 34px">Practices</strong></p>
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  <p><strong>Resting Cell Assay</strong> <p>
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    To test the productivity of different plasmids, resting cell assays were run. All assays were run in a shaker incubator at 32 degrees C and 200 RPM. A phosphate buffered solution was used to suspend the growth of the cultures.  </p>
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  <p><strong>Quantification</strong> <p>
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A GC with a DB5 column was used to quantify data from all assays. For testing ethanol production, the method used had an initial temp. of 60 degrees C, and ramped by 10 degrees C until it reached 250 degrees C, which was then held for 3 minutes.
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Revision as of 20:53, 16 October 2014


 

 

Policy


 

Policy and Practices

text

 


 

Policy and Practices

text

 

 

Practices


 

Resting Cell Assay

To test the productivity of different plasmids, resting cell assays were run. All assays were run in a shaker incubator at 32 degrees C and 200 RPM. A phosphate buffered solution was used to suspend the growth of the cultures.

 

Quantification

A GC with a DB5 column was used to quantify data from all assays. For testing ethanol production, the method used had an initial temp. of 60 degrees C, and ramped by 10 degrees C until it reached 250 degrees C, which was then held for 3 minutes.