Team:Cooper Union/Notebook/Biohack April

From 2014.igem.org

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<pre>
 
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3/13/14
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<h3>3/13/14</h3>
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Digested pBR322 with HindIII.
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<ul><li>Digested pBR322 with HindIII.</li>
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Double digested pBR322 with HindIII and EcoRI.
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<li>Double digested pBR322 with HindIII and EcoRI.</li>
 +
</ul>
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3/27/14
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<h3>3/27/14</h3>
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Ran digests from 3/13/14 on gel.  
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<ul>
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Excised gel fragments with plasmid in them.
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  <li>Ran digests from 3/13/14 on gel. </li>
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Purified gel fragments.
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  <li>Excised gel fragments with plasmid in them.</li>
 +
  <li>Purified gel fragments.</li>
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</ul>
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4/10/14
+
<h3>4/10/14</h3>
Phosphatase treated the digested and double digested pBR322 from 3/13/14.
Phosphatase treated the digested and double digested pBR322 from 3/13/14.
4/17/14
4/17/14
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Digested UV, HSP, and GFP g-blocks with HindIII and EcoRI.  
+
<ul>
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Performed five ligations:
+
  <li>Digested UV, HSP, and GFP g-blocks with HindIII and EcoRI. </li>
-
pBR322 with UV
+
  <li>Performed five ligations:
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pBR322 with HSP
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<dl><dd>pBR322 with UV</dd>
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pBR322 with GFP
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<dd>pBR322 with HSP</dd>
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UV with GFP
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<dd>pBR322 with GFP</dd>
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HSP with GFP
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<dd>UV with GFP</dd>
 +
<dd>HSP with GFP</dd></dl>
 +
</li>
 +
</ul>
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4/24/14
 
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Purified UV+GFP and HSP+GFP ligations.
 
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Digested the two ligations with HindIII.
 
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Ligated each one into pBR322.
 
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</pre>
+
<h3>4/24/14</h3>
 +
<ul>
 +
  <li>Purified UV+GFP and HSP+GFP ligations.</li>
 +
  <li>Digested the two ligations with HindIII.</li>
 +
  <li>Ligated each one into pBR322.</li>
 +
</ul>

Revision as of 20:35, 16 October 2014

Cooper Union 2014 iGEM

Biohacker Kit

PRE MAY MAY JUN JUL


3/13/14

  • Digested pBR322 with HindIII.
  • Double digested pBR322 with HindIII and EcoRI.

3/27/14

  • Ran digests from 3/13/14 on gel.
  • Excised gel fragments with plasmid in them.
  • Purified gel fragments.

4/10/14

Phosphatase treated the digested and double digested pBR322 from 3/13/14. 4/17/14
  • Digested UV, HSP, and GFP g-blocks with HindIII and EcoRI.
  • Performed five ligations:
    pBR322 with UV
    pBR322 with HSP
    pBR322 with GFP
    UV with GFP
    HSP with GFP

4/24/14

  • Purified UV+GFP and HSP+GFP ligations.
  • Digested the two ligations with HindIII.
  • Ligated each one into pBR322.


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