Team:INSA-Lyon/DataPage

From 2014.igem.org

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Revision as of 20:35, 16 October 2014

Curly'on - IGEM 2014 INSA-LYON

DATA PAGE

Data for our favorite new parts

1.Main page - P70-csgA, Wild-type csgA under control of the short curli promoter K342000. This part enables the production curlis, promoting biofilm formation.

2.Main page - P70-csgA-His,single His-tagged csgA under control of the short curli promoter K342000. This part enables the production curlis, promoting biofilm formation, and the single His tag improves nickel capture

3.Main page - P70-csgA-His2, double His-tagged csgA under control of the short curli promoter K342000. This part enables the production curlis, promoting biofilm formation, and the double His tag further improves nickel capture compared to K1404007.

Data for pre-existing parts

1.Main page - P70, short curli promoter. We characterised the expression of the short curli promoter deposited on the registry and compared it with the full curli intergenic region

We've also characterized the following parts

These parts are a continuation of the iGEM 2011 "Cobalt buster" project

1.Main page - Ptac-NiCoTB-rcn-csgBAEFG, device transforming E. coli into a nickel and cobalt biofilter. This part increases the internalization of cobalt and nickel and promotes biofilm formation

2.Main page - Ptac-NiCoTB, constitutive nickel and cobalt transporter generator. This part increases the internalization of cobalt and nickel

@@ Line 30: / Line 30: @@
 <h6 align="left">Data for our favorite new parts</h6>
-<p>1.<a href="http://partsregistry.org/Part:BBa_K1404006">Main page</a> - <b><i>P70-csgA</i>, cobalt-inducible promoter controlling a curli-generating gene, BBa_K540000</b>. This part enable production of cobalt induced curlis, which induces the formation of a biofilm.<br/></p>
+<p>1.<a href="http://partsregistry.org/Part:BBa_K1404006">Main page</a> - <b><i>P70-csgA</i>, Wild-type <i>csgA</i> under control of the short curli promoter K342000</b>. This part enables the production curlis, promoting biofilm formation.</p>
-<p>2.<a href="http://partsregistry.org/Part:BBa_K1404007">Main page</a> - <b><i>P70-csgA-His</i>, cobalt-inducible promoter, BBa_K540001 </b>. This promoter increases the production of a gfp placed under its control when cobalt is in the medium.</p>
+<p>2.<a href="http://partsregistry.org/Part:BBa_K1404007">Main page</a> - <b><i>P70-csgA-His</i>,single His-tagged <i>csgA</i> under control of the short curli promoter K342000</b>. This part enables the production curlis, promoting biofilm formation, and the single His tag improves nickel capture</p>
-<p>3.<a href="http://partsregistry.org/Part:BBa_K1404008">Main page</a> - <b><i>P70-csgA-His2</i>, cobalt-inducible promoter, BBa_K540001 </b>. This promoter increases the production of a gfp placed under its control when cobalt is in the medium.</p>
+<p>3.<a href="http://partsregistry.org/Part:BBa_K1404008">Main page</a> - <b><i>P70-csgA-His2</i>, double His-tagged <i>csgA</i> under control of the short curli promoter K342000</b>. This part enables the production curlis, promoting biofilm formation, and the double His tag further improves nickel capture compared to K1404007. </p>
 </br>
@@ Line 45: / Line 45: @@
 <p>These parts are a continuation of the iGEM 2011 "Cobalt buster" project</p>
 <p>1.<a href="http://partsregistry.org/Part:BBa_K1404001">Main page</a> - <b><i>Ptac-NiCoTB-rcn-csgBAEFG</i>, device transforming E. coli into a nickel and cobalt biofilter</b>. This part increases the internalization of cobalt and nickel and promotes biofilm formation</p>
-<p>1.<a href="http://partsregistry.org/Part:BBa_K1404002">Main page</a> - <b><i>Ptac-NiCoTB</i>, constitutive nickel and cobalt transporter generator</b>. This part increases the internalization of cobalt and nickel</p>
+<p>2.<a href="http://partsregistry.org/Part:BBa_K1404002">Main page</a> - <b><i>Ptac-NiCoTB</i>, constitutive nickel and cobalt transporter generator</b>. This part increases the internalization of cobalt and nickel</p>
 </br>