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Team:INSA-Lyon/Parts
From 2014.igem.org
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<p>On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction.</br> | <p>On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction.</br> | ||
| - | The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA.</br> | + | The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA-WT.</br> |
| - | The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called His1.</br> | + | The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called CsgA-His1.</br> |
| - | The last CsgA is almost the same but with 2 his tag. This construction is called His2.</br> | + | The last CsgA is almost the same but with 2 his tag. This construction is called CsgA-His2.</br> |
Revision as of 20:29, 16 October 2014
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PROJECT
PROJECT- PROJECT SUMMARY
- ACHIEVEMENTS
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WETLAB
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MODELING
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HUMAN PRACTICE
HUMAN
PRACTICE- HUMAN PRACTICE SUMMARY
- SYNBIO PERCEPTION
- PROJECT'S INTEREST
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TEAM
5 biobricks have been constructed, three for Nickel chelation, three for promoter characterization.
Biobricks for nickel chelationThe principal of the construction is shown in the figure below.
On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction. The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA-WT. The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called CsgA-His1. The last CsgA is almost the same but with 2 his tag. This construction is called CsgA-His2.
