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Team:StanfordBrownSpelman/Cellulose Cross Linker
From 2014.igem.org
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<h6>More methods here. | <h6>More methods here. | ||
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| + | <div class="small-7 small-centered columns"><br><center><img src="https://2014.igem.org/File:Cellulose_cross_linker.png"><br> | ||
| + | <h6><center>Image description goes here.</center></h6> | ||
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| - | <div class="sub4"><a href="work/PUT-PDF-REFERENCE-HEREpdf" target="_blank"><img src=" | + | <div class="sub4"><a href="work/PUT-PDF-REFERENCE-HEREpdf" target="_blank"><img src=""></a><a href="work/PUT-PDF-REFERENCE-HEREpdf">Click here to go to our project journal, which details our design and engineering process and included descriptions of the protocols we developed and used.</a></div> |
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<h5><center>Results</h5> | <h5><center>Results</h5> | ||
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| - | Our initial approach was to include two identical cellulose-binding domains on either side of the streptavidin domain. | + | Our initial approach was to include two identical cellulose-binding domains on either side of the streptavidin domain. Due to the repetitive nature of the sequence and potential homologous recombination, we had many issues with molecular cloning. We changed our approach to using two different cellulose-binding domains with different sequences. This allowed us to successfully conduct the molecular cloning. |
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Revision as of 19:44, 16 October 2014
Cellulose Cross-Linker
The goal of this subproject is to create a cellulose cross-linking protein to increase material strength and allow for the modular attachment of biological sensors. This fusion protein contains two distinct cellulose-binding domains[1] on either side of a streptavidin domain. The cellulose-binding domains cross link the cellulose fibers while the streptavidin serves as a binding domain for biological sensors. The interaction between SA (streptavidin) and biotin is one of the strongest non-covalent interactions in nature [2]. Therefore a cell expressing an outer membrane protein that has been biotinylated will bind tightly to this domain. This will allow our UAV to make use of a number of biological sensors.
The goal of this subproject is to create a cellulose cross-linking protein to increase material strength and allow for the modular attachment of biological sensors. This fusion protein contains two distinct cellulose-binding domains[1] on either side of a streptavidin domain. The cellulose-binding domains cross link the cellulose fibers while the streptavidin serves as a binding domain for biological sensors. The interaction between SA (streptavidin) and biotin is one of the strongest non-covalent interactions in nature [2]. Therefore a cell expressing an outer membrane protein that has been biotinylated will bind tightly to this domain. This will allow our UAV to make use of a number of biological sensors.
Methods
Methods here.
Figure 1. Figure caption here.
More methods here.
