Revision as of 18:38, 8 July 2014

Notebook : July 8

Sean

Protocol:

Mathieu & Maher

Romain & Sean

Plasmids used:

E. coli strain used:

@@ Line 3: / Line 3: @@
 =='''Lab work'''==
-===='''1 - Transformation in DH5α of the following biobricks:'''====
+===='''1 - Rehydration of the following biobricks:'''====
-Mathieu & Maher
+'''Sean'''
+* BBa B0015(CmR)
+* BBa J23119(CmR)
+* BBa K731020(CmR)
+* BBa K206000(CmR)
+* BBa J04500(CmR)
+* BBa J23100(AmpR)
+* BBa J23106(AmpR)
+* BBa J23114(AmpR)
+Protocol:
+# Pierce foil cover of well containing biobrick of interest.
+# Add 10 μL of water in well.
+# Transfer contents of well into a 1.5 mL microcentrifuge tube.
+===='''2 - Transformation in DH5α of the following biobricks:'''====
+'''Mathieu & Maher'''
 * BBa B0015(CmR)
 * BBa J23119(CmR)
@@ Line 17: / Line 35: @@
 * control -
-Protocol : [https://2013.igem.org/Team:Paris_Saclay/Protocols/Transformation]
+[https://2013.igem.org/Team:Paris_Saclay/Protocols/Transformation Protocol]
+===='''3 - Plasmid DNA preparation:'''====
+'''Romain & Sean'''
+Plasmids used:
+* pJBEI-6409
+''E. coli'' strain used:
+* XL1-Blue