"
Team:Zamorano/Biosecurity
From 2014.igem.org
(Difference between revisions)
| Line 1,164: | Line 1,164: | ||
<td width="72" nowrap="nowrap" style="width:54.1pt;padding:0cm 3.5pt 0cm 3.5pt;height:8.05pt;"></td> | <td width="72" nowrap="nowrap" style="width:54.1pt;padding:0cm 3.5pt 0cm 3.5pt;height:8.05pt;"></td> | ||
<td width="86" nowrap="nowrap" style="width:64.2pt;padding:0cm 3.5pt 0cm 3.5pt;height:8.05pt;"></td> | <td width="86" nowrap="nowrap" style="width:64.2pt;padding:0cm 3.5pt 0cm 3.5pt;height:8.05pt;"></td> | ||
| - | <td width="451" nowrap="nowrap" colspan="4" style="width:338.5pt;background:#EEECE1;padding:0cm 3.5pt 0cm 3.5pt;height:8.05pt;" bgcolor="#EEECE1"><p class="MsoNormal" align="center" style="text-align:center;"><strong><span style=" | + | <td width="451" nowrap="nowrap" colspan="4" style="width:338.5pt;background:#EEECE1;padding:0cm 3.5pt 0cm 3.5pt;height:8.05pt;" bgcolor="#EEECE1"><p class="MsoNormal" align="center" style="text-align:center;"><strong><span style="font-size:12.0pt; color:black; ">Risk estimation</span></strong></p></td> |
</tr> | </tr> | ||
<tr style="height:11.0pt;"> | <tr style="height:11.0pt;"> | ||
| - | <td width="72" nowrap="nowrap" rowspan="4" style="width:54.1pt;border:solid white 1.0pt;background:#EEECE1;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#EEECE1"><p class="MsoNormal" align="center" style="text-align:center;"><strong><span style=" | + | <td width="72" nowrap="nowrap" rowspan="4" style="width:54.1pt;border:solid white 1.0pt;background:#EEECE1;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#EEECE1"><p class="MsoNormal" align="center" style="text-align:center;"><strong><span style="font-size:12.0pt; color:black; ">Probability</span></strong></p></td> |
| - | <td width="86" nowrap="nowrap" style="width:64.2pt;border:solid white 1.0pt;border-left:none;background:#EEECE1;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#EEECE1"><p class="MsoNormal" align="center" style="text-align:center;"><span style=" | + | <td width="86" nowrap="nowrap" style="width:64.2pt;border:solid white 1.0pt;border-left:none;background:#EEECE1;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#EEECE1"><p class="MsoNormal" align="center" style="text-align:center;"><span style="color:black; ">Very high</span></p></td> |
| - | <td width="125" nowrap="nowrap" style="width:93.75pt;border:solid white 1.0pt;border-left:none;background:#C4D79B;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#C4D79B"><p class="MsoNormal" align="center" style="text-align:center;"><span style=" | + | <td width="125" nowrap="nowrap" style="width:93.75pt;border:solid white 1.0pt;border-left:none;background:#C4D79B;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#C4D79B"><p class="MsoNormal" align="center" style="text-align:center;"><span style="color:black; ">Low</span></p></td> |
<td width="125" nowrap="nowrap" style="width:93.75pt;border:solid white 1.0pt;border-left:none;background:#FDE9D9;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FDE9D9"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">Moderate</span></p></td> | <td width="125" nowrap="nowrap" style="width:93.75pt;border:solid white 1.0pt;border-left:none;background:#FDE9D9;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FDE9D9"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">Moderate</span></p></td> | ||
| - | <td width="106" nowrap="nowrap" style="width:79.5pt;border:solid white 1.0pt;border-left:none;background:#FABF8F;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FABF8F"><p class="MsoNormal" align="center" style="text-align:center;"><span style=" | + | <td width="106" nowrap="nowrap" style="width:79.5pt;border:solid white 1.0pt;border-left:none;background:#FABF8F;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FABF8F"><p class="MsoNormal" align="center" style="text-align:center;"><span style="color:black; ">High</span></p></td> |
| - | <td width="95" nowrap="nowrap" style="width:71.5pt;border:solid white 1.0pt;border-left:none;background:#FABF8F;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FABF8F"><p class="MsoNormal" align="center" style="text-align:center;"><span style=" | + | <td width="95" nowrap="nowrap" style="width:71.5pt;border:solid white 1.0pt;border-left:none;background:#FABF8F;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FABF8F"><p class="MsoNormal" align="center" style="text-align:center;"><span style="color:black; ">High</span></p></td> |
</tr> | </tr> | ||
<tr style="height:11.0pt;"> | <tr style="height:11.0pt;"> | ||
| - | <td width="86" nowrap="nowrap" style="width:64.2pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#EEECE1;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#EEECE1"><p class="MsoNormal" align="center" style="text-align:center;"><span style=" | + | <td width="86" nowrap="nowrap" style="width:64.2pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#EEECE1;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#EEECE1"><p class="MsoNormal" align="center" style="text-align:center;"><span style="color:black; ">High</span></p></td> |
| - | <td width="125" nowrap="nowrap" style="width:93.75pt;border:none;border-right:solid white 1.0pt;background:#C4D79B;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#C4D79B"><p class="MsoNormal" align="center" style="text-align:center;"><span style=" | + | <td width="125" nowrap="nowrap" style="width:93.75pt;border:none;border-right:solid white 1.0pt;background:#C4D79B;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#C4D79B"><p class="MsoNormal" align="center" style="text-align:center;"><span style="color:black; ">Low</span></p></td> |
| - | <td width="125" nowrap="nowrap" style="width:93.75pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#C4D79B;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#C4D79B"><p class="MsoNormal" align="center" style="text-align:center;"><span style=" | + | <td width="125" nowrap="nowrap" style="width:93.75pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#C4D79B;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#C4D79B"><p class="MsoNormal" align="center" style="text-align:center;"><span style="color:black; ">Low</span></p></td> |
| - | <td width="106" nowrap="nowrap" style="width:79.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#FDE9D9;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FDE9D9"><p class="MsoNormal" align="center" style="text-align:center;"><span style=" | + | <td width="106" nowrap="nowrap" style="width:79.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#FDE9D9;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FDE9D9"><p class="MsoNormal" align="center" style="text-align:center;"><span style="color:black; ">Moderate</span></p></td> |
| - | <td width="95" nowrap="nowrap" style="width:71.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#FABF8F;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FABF8F"><p class="MsoNormal" align="center" style="text-align:center;"><span style=" | + | <td width="95" nowrap="nowrap" style="width:71.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#FABF8F;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FABF8F"><p class="MsoNormal" align="center" style="text-align:center;"><span style="color:black; ">High</span></p></td> |
</tr> | </tr> | ||
<tr style="height:11.0pt;"> | <tr style="height:11.0pt;"> | ||
| - | <td width="86" nowrap="nowrap" style="width:64.2pt;border:none;border-bottom:solid white 1.0pt;background:#FFFF99;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FFFF99"><p class="MsoNormal" align="center" style="text-align:center;"><span style=" | + | <td width="86" nowrap="nowrap" style="width:64.2pt;border:none;border-bottom:solid white 1.0pt;background:#FFFF99;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FFFF99"><p class="MsoNormal" align="center" style="text-align:center;"><span style="color:black;">Low</span></p></td> |
| - | <td width="125" nowrap="nowrap" style="width:93.75pt;border:solid red 1.5pt;background:#B8CCE4;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#B8CCE4"><p class="MsoNormal" align="center" style="text-align:center;"><span style=" | + | <td width="125" nowrap="nowrap" style="width:93.75pt;border:solid red 1.5pt;background:#B8CCE4;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#B8CCE4"><p class="MsoNormal" align="center" style="text-align:center;"><span style="color:red; ">Insignificant</span></p></td> |
| - | <td width="125" nowrap="nowrap" style="width:93.75pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#C4D79B;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#C4D79B"><p class="MsoNormal" align="center" style="text-align:center;"><span style=" | + | <td width="125" nowrap="nowrap" style="width:93.75pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#C4D79B;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#C4D79B"><p class="MsoNormal" align="center" style="text-align:center;"><span style="color:black; ">Low</span></p></td> |
| - | <td width="106" nowrap="nowrap" style="width:79.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#FDE9D9;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FDE9D9"><p class="MsoNormal" align="center" style="text-align:center;"><span style=" | + | <td width="106" nowrap="nowrap" style="width:79.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#FDE9D9;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FDE9D9"><p class="MsoNormal" align="center" style="text-align:center;"><span style="color:black; ">Moderate</span></p></td> |
<td width="95" nowrap="nowrap" style="width:71.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#FDE9D9;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FDE9D9"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">Moderate</span></p></td> | <td width="95" nowrap="nowrap" style="width:71.5pt;border-top:none;border-left:none;border-bottom:solid white 1.0pt;border-right:solid white 1.0pt;background:#FDE9D9;padding:0cm 3.5pt 0cm 3.5pt;height:11.0pt;" bgcolor="#FDE9D9"><p class="MsoNormal" align="center" style="text-align:center;"><span style="font-family:'Book Antiqua','serif'; color:black; ">Moderate</span></p></td> | ||
</tr> | </tr> | ||
Revision as of 17:34, 16 October 2014
Risk assessment of the E. coli bacteria modified with genes of fluorescence
Biosafety
Good Security Practices (GSP) were taken of a publishing of the Worldwide Health Organization (WHO) titled “Biosecurity manual in the lab” in the 2005 and adapted to the practice made. This guaranteed the entire safety of the staff members that worked in the modification and testing of the bacteria. It is important to mention that although the organism does not represent a biologic risk, for the development of this Project it was necessary to operate under a biosafety level 2 with the intention of learning how to apply better the GSP. In order to achieve this objective the practice was based in 4 fundamental axes of biosafety.
- Access restricted to people not authorized.
- Personal protection equipment team manages the instruments in the lab.
- Biocontention mechanisms.
- Final disposal methods.
Risk estimation |
|||||
Probability |
Very high |
Low |
Moderate |
High |
High |
High |
Low |
Low |
Moderate |
High |
|
Low |
Insignificant |
Low |
Moderate |
Moderate |
|
Very Low |
Insignificant |
Insignificant |
Low |
Moderate |
|
|
|
Marginal |
Minor |
Intermedia |
Mayor |
|
|
Consequence |
|||
Thanks to the GSP and other basic measures of biosecurity the happening probability estimation and the consequence of this to happen be meaningless.
