Purpose

The difference in the biology of plants unveils a need for new standards. We decided to write a Request for Comments (RFC) to unite the plant SynBio community by defining a common language for plant synthetic biology, extensible to all other Eukaryotes. Its syntax is based on the Golden Gate System¹ cloning method which uses TypeIIS restriction enzymes; variations, such as GB2.0² and MoClo³, are already being used across the field. This RFC brings together their common features and sets a consensus across the plant field for construct assembly and part repositories.

Marchantia polymorpha, a primitive liverwort, is being established as a new model organism for plant Synthetic Biology^{4; Contrary to historical model species, the choice of M. polymorpha has preceded its establishment. This is an opportunity to set standards in a coherent and united way paving the way for faster and more efficient sharing of information and parts.}

The fewer the rules defining a standard, the more widely that standard is adopted. The introduction of this new chassis with its novel tool kit is the occasion to allow more flexibility in the Registry⁵, opening it to wider usage and contributions.

Thank you to the UEA and Valencia iGEM teams for their collaborations.

Rise of the Titles

Third part here