Team:Colombia/How to parts

From 2014.igem.org

(Difference between revisions)
 
(7 intermediate revisions not shown)
Line 1: Line 1:
{{Http://2014.igem.org/Team:Colombia Uniandes/Bootstrap}}
{{Http://2014.igem.org/Team:Colombia Uniandes/Bootstrap}}
-
 
<html>
<html>
<div class="container">
<div class="container">
Line 8: Line 7:
</html>
</html>
{| border="2" align="center"
{| border="2" align="center"
-
|+'''Primers used to construct Nicko and Chimi'''
+
|+'''Primers used to construct XXXXXXX'''
| style="text-align: center;" |Name
| style="text-align: center;" |Name
| style="text-align: center;" |Sequence
| style="text-align: center;" |Sequence
-
| style="text-align: center;" |Identification Number
 
|-  
|-  
-
| style="text-align: left;" |FW XbaI-BAP2
+
| style="text-align: left;" |FW pqrr4     
-
| style="text-align: left;" |CAC CTC TAG AGA GCA TTA ATG CAA GTC GAG AAA GG
+
| style="text-align: left;" |CTT GTC TAG ATT GCA TTT TGC AAA TTT TG
-
| style="text-align: center;" |19
+
|-  
|-  
-
| style="text-align: left;" |FW XbaI-GAL1
+
| style="text-align: left;" |RV pqrr4
-
| style="text-align: left;" |CAC CTC TAG AGG CTA TAT TGA AGT ACG GAT TAG
+
| style="text-align: left;" |GGT CAC TAG TCA ATC TAA TAA ATG CAG ATT
-
| style="text-align: center;" |15
+
|-  
|-  
-
| style="text-align: left;" |FW Fusion GAL1-KOZAC-mCherry
+
| style="text-align: left;" |FW LuxOD47E
-
| style="text-align: left;" |GGA AAC TAG ACC CGC CGC CAC CAT GGA GAT GGT GAG CAA GGG CGA GG
+
| style="text-align: left;" |CAA TTC TAG AAA AGA GGA GAA ATA CTA GAT G
-
| style="text-align: center;" |17
+
|-
|-
-
| style="text-align: left;" |RV Fusion GAL1-KOZAC-mCherry
+
| style="text-align: left;" |RV LuxOD47E
-
| style="text-align: left;" |CCT CGC CCT TGC TCA CCA TCT CCA TGG TGG CGG CGG GTC TAG TTT CC
+
| style="text-align: left;" |ATA TAC TAG TTG TGA CTC TAG TAG AGA
-
| style="text-align: center;" |13
+
|}
 +
 
 +
<br><br>
 +
{| border="2" align="center"
 +
|+'''BioBricks used to construct XXXXXXX'''
 +
| style="text-align: center;" |Name
 +
| style="text-align: center;" |Description
 +
| style="text-align: center;" |Developer team
 +
 
 +
|-
 +
| style="text-align: left;" |[http://parts.igem.org/Part:BBa_K581010 BBa_K581010]   
 +
| style="text-align: left;" |Regulatory proteins for receiving signaling of CAI-1
 +
| style="text-align: left;" |Peking_Signaling 2011
 +
 
 +
|-
 +
| style="text-align: left;" |[http://parts.igem.org/Part:BBa_K581011 BBa_K581011]
 +
| style="text-align: left;" |A generator of CAI-1 behind pBAD promoter
 +
| style="text-align: left;" |Peking_Signaling 2011
 +
 
 +
|-
 +
| style="text-align: left;" |[http://parts.igem.org/Part:BBa_K581009 BBa_K581009]
 +
| style="text-align: left;" |GFP behind promoter qrr
 +
| style="text-align: left;" |Peking_Signaling 2011
 +
 
|-
|-
-
| style="text-align: left;" |FW Fusion BAP2-KOZAC-GAL4
+
| style="text-align: left;" |[http://parts.igem.org/Part:BBa_K218017 BBa_K218017]
-
| style="text-align: left;" |CTC AAG TGA TTT TAG AAC CCG CCG CCA CCA TGG AGA TGA AGC TAC TGT CTT C
+
| style="text-align: left;" |LuxO D47E under constitutive expression of TetR repressible promoter
-
| style="text-align: center;" |11
+
| style="text-align: left;" |Calgary 2009
|-
|-
-
| style="text-align: left;" |RV Fusion BAP2-KOZAC-GAL4
+
| style="text-align: left;" |[http://parts.igem.org/Part:BBa_K592025 BBa_K592025]
-
| style="text-align: left;" |GAA GAC AGT AGC TTC ATC TCC ATG GTG GCG GCG GGT TCT AAA ATC ACT TGA G
+
| style="text-align: left;" |Purple-blue chromoprotein amilCP
-
| style="text-align: center;" |3
+
| style="text-align: left;" |Uppsala-Sweden 2011
 +
 
|-
|-
-
| style="text-align: left;" |FW FUSION GAL4-VP16-1
+
| style="text-align: left;" |[http://parts.igem.org/Part:BBa_K518012 BBa_K518012]
-
| style="text-align: left;" |CAG TTG ACT GTA TCT TCG AGG TCG ACC ATT CAG ATC TCA CGA GCC GCC CCC CCG ACC G
+
| style="text-align: left;" |RFP
-
| style="text-align: center;" |1
+
| style="text-align: left;" |UT-Tokyo 2011
 +
 
|-
|-
-
| style="text-align: left;" |RV FUSION GAL4-VP16-1
+
| style="text-align: left;" |[http://parts.igem.org/Part:BBa_K206000 BBa_K206000]
-
| style="text-align: left;" |CGG TCG GGG GGG CGG CTC GTG AGA TCT GAA TGG TCG ACC TCG AAG ATA CAG TCA ACT G
+
| style="text-align: left;" |pBAD strong(arabinose inducible promoter)
-
| style="text-align: center;" |5
+
| style="text-align: left;" |UT-Tokyo British_Columbia 2009
-
|-
+
 
-
| style="text-align: left;" |FW FUSION VP6-GCR
+
-
| style="text-align: left;" |CCT ACG GCG CTC TGG ATA TGA TCT CTC GAG CCA TTC AGC AAG CCA CTG CAG G
+
-
| style="text-align: center;" |9
+
-
|-
+
-
| style="text-align: left;" |RV FUSION VP6-GCR
+
-
| style="text-align: left;" |CCT GCA GTG GCT TGC TGA ATG GCT CGA GAG ATC ATA TCC AGA GCG CCG TAG G
+
-
| style="text-align: center;" |6
+
-
|-
+
-
| style="text-align: left;" |FW Fusion GCR-yeastTerminador
+
-
| style="text-align: left;" |GCT TCT GTT TCA TCA AAA ATG AAA AGT GAA TAT CCA AAC GAG AAT GTC AAT GG
+
-
| style="text-align: center;" |35
+
-
|-
+
-
| style="text-align: left;" |RV Fusion GCR-yeastTerminador
+
-
| style="text-align: left;" |CCA TTG ACA TTC TCG TTT GGA TAT TCA CTT TTC ATT TTT GAT GAA ACA GAA GC
+
-
| style="text-align: center;" |34
+
-
|-
+
-
| style="text-align: left;" |FW Fusion mCherry-yeastTerminador
+
-
| style="text-align: left;" |GGA CGA GCT GTA CAA GTA ATA AAA AGT GAA TAT CCA AAC GAG AAT GTC AAT GG
+
-
| style="text-align: center;" |33
+
-
|-
+
-
| style="text-align: left;" |RV Fusion mCherry-yeastTerminador
+
-
| style="text-align: left;" |CCA TTG ACA TTC TCG TTT GGA TAT TCA CTT TTT ATT ACT TGT ACA GCT CGT CC
+
-
| style="text-align: center;" |32
+
-
|-
+
-
| style="text-align: left;" |FW Constructo final
+
-
| style="text-align: left;" |ACC GTC TAG ATT GAC AGC TAG CTC AGT CCT AGG
+
-
| style="text-align: center;" |40
+
-
|-
+
-
| style="text-align: left;" |FW HoxN
+
-
| style="text-align: left;" |ATG GAA GCA CTG CCG AAC GAT T
+
-
| style="text-align: center;" |42
+
-
|-
+
-
| style="text-align: left;" |RV HoxNconColamRfP
+
-
| style="text-align: left;" |GTC TTC GGA GGA AGC CAT CTA TTC GAC GAA CCG
+
-
| style="text-align: center;" |44
+
-
|-
+
-
| style="text-align: left;" |FW mRFPconColaHoxN
+
-
| style="text-align: left;" |GTT CGT CGA ATA GAT GGC TTC CTC CGA AGA
+
-
| style="text-align: center;" |41
+
-
|-
+
-
| style="text-align: left;" |RV mRFPconXba
+
-
| style="text-align: left;" |ACT ATC TAG ATA TAA ACG CAG AAA GGC CCA CCC
+
-
| style="text-align: center;" |38
+
-
|-
+
-
| style="text-align: left;" |RcnR+B1006 RV
+
-
| style="text-align: left;" |AAA AAC CCC GCC CTG TCA GGG GCG GGG TTT TTT TTT TTA TTT GAT ATA TGA ATC CAG CAC
+
-
| style="text-align: center;" |10
+
-
|-
+
-
| style="text-align: left;" |FW Prcna
+
-
| style="text-align: left;" |GCG GGG TTT TTT TTA CGG ATT GTA TGA GAC AT
+
-
| style="text-align: center;" |43
+
-
|-
+
-
| style="text-align: left;" |RV Prcna
+
-
| style="text-align: left;" |TCC ATT TTC TCC TCT TTG ATA ATA ATT CTT AGT AT
+
-
| style="text-align: center;" |39
+
-
|-
+
-
| style="text-align: left;" |J23117+B0034+RcnR FW
+
-
| style="text-align: left;" |GAC AGC TAG CTC AGT CCT AGG GAT TGT GCT AGC AAA GAG GAG AAA ATG TCT CAT ACA ATC
+
-
| style="text-align: center;" |23
+
-
|-
+
-
| style="text-align: left;" |J23117+B0034+RcnR RV
+
-
| style="text-align: left;" |GAT TGT ATG AGA CAT TTT CTC CTC TTT GCT AGC ACA ATC CCT AGG ACT GAG CTA GCT GTC
+
-
| style="text-align: center;" |21
+
|-
|-
 +
| style="text-align: left;" |[http://parts.igem.org/Part:BBa_Q04400 BBa_Q04400]
 +
| style="text-align: left;" |TetR QPI (inverter)
 +
| style="text-align: left;" |Antiquity 2003
|}
|}
 +
<html>
<html>
</div>
</div>
</div>
</div>
</html>
</html>

Latest revision as of 02:10, 8 July 2014

How to parts


Primers used to construct XXXXXXX
Name Sequence
FW pqrr4 CTT GTC TAG ATT GCA TTT TGC AAA TTT TG
RV pqrr4 GGT CAC TAG TCA ATC TAA TAA ATG CAG ATT
FW LuxOD47E CAA TTC TAG AAA AGA GGA GAA ATA CTA GAT G
RV LuxOD47E ATA TAC TAG TTG TGA CTC TAG TAG AGA



BioBricks used to construct XXXXXXX
Name Description Developer team
[http://parts.igem.org/Part:BBa_K581010 BBa_K581010] Regulatory proteins for receiving signaling of CAI-1 Peking_Signaling 2011
[http://parts.igem.org/Part:BBa_K581011 BBa_K581011] A generator of CAI-1 behind pBAD promoter Peking_Signaling 2011
[http://parts.igem.org/Part:BBa_K581009 BBa_K581009] GFP behind promoter qrr Peking_Signaling 2011
[http://parts.igem.org/Part:BBa_K218017 BBa_K218017] LuxO D47E under constitutive expression of TetR repressible promoter Calgary 2009
[http://parts.igem.org/Part:BBa_K592025 BBa_K592025] Purple-blue chromoprotein amilCP Uppsala-Sweden 2011
[http://parts.igem.org/Part:BBa_K518012 BBa_K518012] RFP UT-Tokyo 2011
[http://parts.igem.org/Part:BBa_K206000 BBa_K206000] pBAD strong(arabinose inducible promoter) UT-Tokyo British_Columbia 2009
[http://parts.igem.org/Part:BBa_Q04400 BBa_Q04400] TetR QPI (inverter) Antiquity 2003

Retrieved from "http://2014.igem.org/Team:Colombia/How_to_parts"