Team:USyd-Australia/Notebook

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Latest revision as of 15:20, 16 October 2014

Notebook
Date	Day	Activity	Who?
15/5	Thursday	Preparation of common solutions: LB Stock Streptomycin (Sm) 200mg/mL, Chloramphenicol (Cm) 25 (mg/mL) Sm200 and Cm25 plates	Callum and Jeanne
16/5	Friday	Preparation of common solutions: Stock Kanamycin (Km) 50 (mg/ml) Km50 plates	Abi and Tom
22/5	Thursday	Preparation of common solutions: 2M NaOH Stock Trimethoprim (Tm) 50mg/mL Restriction digest: pK18 digested with HindIII, EcoRI, BamHI Agarose Gel Electrophoresis: pK18 digests and undigested DNA Post-stained with GelRed Results...?	Callum and Jeanne
23/5	Friday	Growing up gene source E. coli JM109 containing pK18, for gene ____, on LB-Km _____ containing R388, for gene ____, on LB-Tm JM109 containing pUS44, for Pc-AttI1, on LB-Sm Transform pUS41 by heat shock into Top10, grow on Sm (in hindsight, Top10 already has Sm resistance, so this was not valid) One Loopful of each culture from glycerol stocks was streaked onto appropriate selective media under aseptic conditions. All plates were incubated at 30 degrees over the weekend.	Abi and Tom

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