"
3/13/14 Digested pBR322 with HindIII. Double digested pBR322 with HindIII and EcoRI. 3/27/14 Ran digests from 3/13/14 on gel. Excised gel fragments with plasmid in them. Purified gel fragments. 4/10/14 Phosphatase treated the digested and double digested pBR322 from 3/13/14. 4/17/14 Digested UV, HSP, and GFP g-blocks with HindIII and EcoRI. Performed five ligations: pBR322 with UV pBR322 with HSP pBR322 with GFP UV with GFP HSP with GFP 4/24/14 Purified UV+GFP and HSP+GFP ligations. Digested the two ligations with HindIII. Ligated each one into pBR322.
Team:Cooper Union/Notebook/Biohack April
From 2014.igem.org
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Revision as of 14:08, 16 October 2014
