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==Introduction== In our project we dig into the fascinating world of small RNA (sRNA) regulation with the aim of engeneering a so-called trap sRNA regulating module and hereby creating a universal tool for a whole new way of regulating gene expression in synthetic biology. To fully understand our project, at basic knowledge of sRNAs, the way they regulate gene expression, the players involved (the RNA chaperone Hfq) and the unique features of the regulation is required. So continue reading if these words are new to you. A recurring theme for sRNA regulation is the diversity of its mechanism; some sRNAs are repressors other activators, some act catalytically others non-catalytically, some require Hfq others not and so on. All in all this illustrates the extensive variety of sRNAs.