Team:UB Indonesia/backup
From 2014.igem.org
(Difference between revisions)
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<h2><center>“LAB JOURNAL”</center></h2> | <h2><center>“LAB JOURNAL”</center></h2> | ||
<p align="justify"> | <p align="justify"> | ||
- | <ul> | + | <ul type="circle"> |
- | <li>Preventing</li> | + | <li><b>Preventing</li> |
- | + | A. MAY </b> | |
<ul><li>Week 3</li> | <ul><li>Week 3</li> | ||
Discussion. Discussion about biobrick and what we can do by used this biobrick. | Discussion. Discussion about biobrick and what we can do by used this biobrick. | ||
Line 209: | Line 209: | ||
Discussion. Discussion about project - fix about cervical cancer. | Discussion. Discussion about project - fix about cervical cancer. | ||
</ul> | </ul> | ||
+ | |||
+ | <b>B. JUNE</b> | ||
+ | <ul> | ||
+ | <li>Week 1</li> | ||
+ | Discussion. Discussion about project - fix that we will do research in “preventing, screening and therapy” for cervical cancer. And our sub-team focus in preventing cervical cancer. | ||
+ | <li>Week 2</li> | ||
+ | Bioinformatic. Study bioinformatic and protocol for each project. | ||
+ | <li>Week 3</li> | ||
+ | Bioinformatic. Study bioinformatic for each project and discussion about human practice. | ||
+ | <li>Week 4</li> | ||
+ | Laboratory. Study in laboratory - learn how to work with gene (competent cell, transformation, etc.) | ||
+ | </ul> | ||
+ | |||
+ | <b>C. AUGUST</b> | ||
+ | <ul> | ||
+ | <li>Week 1</li> | ||
+ | Laboratory. Having traine about working in molecular biology, especially in using lab tools (PCR, Electrophoresis, etc.) | ||
+ | <li>Week 2</li> | ||
+ | Discussion. Discussion about each project development, human practice, and future plan | ||
+ | <li>Week 3</li> | ||
+ | Preparation. Competent cell preparation | ||
+ | <li>Week 4</li> | ||
+ | Preparation. Competent cell preparation | ||
+ | </ul> | ||
+ | |||
+ | <b>D. September</b> | ||
+ | <ul> | ||
+ | <li>Week 1</li> | ||
+ | Discussion & Laboratory. Take hela cells from Yogyakarta and did transformation. Cultured hela cell and discuss with Prof Nellen from Kassel university about funding. Prof Nellen also works at molecular biology for school and the public in Kassel University, it is a student club. He told us how to make an event for funding our project, beside he suggess us to have a good relation and collaboration with other university. | ||
+ | <li>Week 2</li> | ||
+ | Discussion. Discussion about protocol and wet lab work timeline. We face a problem to choose what is the best promotor for the siRNA. We confused in using CAMP or U6/H1 promotor. Then we discuss it with our beloved supervisor, Mr. Widodo, he give us other promotor named pUR 6 and ask to check it in NetCutter. | ||
+ | <li>Week 3</li> | ||
+ | Laboratory. Annealing oligonucleotide of siRNA | ||
+ | <li>Week 4</li> | ||
+ | Laboratory. Transformation to DH5α | ||
+ | </ul> | ||
+ | |||
+ | <b>E. OCTOBER</b> | ||
+ | <ul> | ||
+ | <li>Week 1</li> | ||
+ | Laboratory. Cloning and PCR oligonucleotide. | ||
+ | <li>Week 2</li> | ||
+ | Laboratory. Electrophoresis. | ||
+ | </ul> | ||
+ | |||
<li>Therapy</li> | <li>Therapy</li> | ||
<li>Screening</li> | <li>Screening</li> |
Revision as of 12:14, 16 October 2014