Team:Evry/Interlab Study/10-08-2014

From 2014.igem.org

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<h2>Construction n°1: PSB1C3 with I20260</h2>
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<h2>Other constructions of the Anderson library of constitutive promoters</h2>
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<br/><h2>Construction n°2: PSB1C3 with J23101-E1010</h2>
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<i>PSB1C3+E0240+J23119</i> - <i>PSB1C3+E0240+J23117</i> <br>
 
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Measure of the cells' growth and fluorecence on TECAN infiniteM200 with rank of GFP 80-500nM. (11h00-18h00)<br>
 
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<img src="" alt="IMAGE DU PLAN DE PLAQUE" /> <br>
 
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<i>PSB1C3+E0240+J23114</i>: <br>
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Measure of the cells' growth and fluorecence on TECAN infiniteM200 with rank of GFP 80-500nM. (11h00-18h00)<br>
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<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2>  
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<img src="" alt="IMAGE DU PLAN DE PLAQUE" /> <br>
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Measure of the cells' growth and fluorecence on TECAN infiniteM200 with rank of GFP 80-500nM. (12h00-17h00)<br>
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<i>PSB1C3+E0240+J23115</i> - <i>PSB1C3+E0240+J23116</i> <br>
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<img src="https://static.igem.org/mediawiki/2014/a/a4/PlaqueLeftfinal.png" alt="IMAGE DU PLAN DE PLAQUE" /> <br>
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Measure of the cells' growth and fluorecence on TECAN infiniteM200 with rank of GFP 80-500nM. (16h00-23h00)<br>
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<img src="" alt="IMAGE DU PLAN DE PLAQUE" /> <br>
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<br>
<br>
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<i>PSB1C3+E0240+J23105</i>: <br>
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<br/>
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Measure of the cells' growth and fluorecence on TECAN infiniteM200 with rank of GFP 80-500nM. (16h00-23h00)<br>
+
<h2>Other constructions of the Anderson library of constitutive promoters</h2>
-
<img src="" alt="IMAGE DU PLAN DE PLAQUE" /> <br>
+
<br/>
 +
<i>PSB1C3+E0240+J23100</i>: <br>
 +
Measure of the cells' growth and fluorecence on TECAN infiniteM200 with rank of GFP 80-500nM. (12h00-17h00)<br>
 +
<img src="https://static.igem.org/mediawiki/2014/a/a4/PlaqueLeftfinal.png" alt="IMAGE DU PLAN DE PLAQUE" /> <br>
<br>
<br>
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<i>PSB1C3+E0240+J23110</i> - <i>PSB1C3+E0240+J23107</i> - <i>PSB1C3+E0240+J23106</i>: <br>
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<i>PSB1C3+E0240+J23112</i> - <i>PSB1C3+E0240+J23103</i> - <i>PSB1C3+E0240+J23113</i>: <br>
Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h at 37°C. <br>
Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h at 37°C. <br>
 +
Measure of the cells' growth and fluorecence on TECAN infiniteM200 with rank of GFP 80-500nM. (17h00-00h00)<br>
 +
<img src="https://static.igem.org/mediawiki/2014/8/85/112-103-113.png" alt="IMAGE DU PLAN DE PLAQUE" /> <br>
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<i>PSB1C3+E0240+J23108</i> - <i>PSB1C3+E0240+J23118</i> - <i>PSB1C3+E0240+J23111</i> - <i>PSB1C3+E0240+J23102</i>: <br>
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<i>PSB1C3+E0240+J23109</i> - <i>PSB1C3+E0240+J23117</i> - <i>PSB1C3+E0240+J23114</i>: <br>
Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h at 37°C. <br>
Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h at 37°C. <br>
 +
<br>
-
 
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<i>PSB1C3+E0240+J23115</i> - <i>PSB1C3+E0240+J23116</i> - <i>PSB1C3+E0240+J23105</i>: <br>
 +
Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h at 37°C. <br>
 +
<br>
<span class="cd-date">Oct 08</span>  
<span class="cd-date">Oct 08</span>  

Latest revision as of 12:02, 16 October 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010


Measure of the cells' growth and fluorecence on TECAN infiniteM200 with rank of GFP 80-500nM. (12h00-17h00)
IMAGE DU PLAN DE PLAQUE


Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23100:
Measure of the cells' growth and fluorecence on TECAN infiniteM200 with rank of GFP 80-500nM. (12h00-17h00)
IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23112 - PSB1C3+E0240+J23103 - PSB1C3+E0240+J23113:
Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h at 37°C.
Measure of the cells' growth and fluorecence on TECAN infiniteM200 with rank of GFP 80-500nM. (17h00-00h00)
IMAGE DU PLAN DE PLAQUE

PSB1C3+E0240+J23109 - PSB1C3+E0240+J23117 - PSB1C3+E0240+J23114:
Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h at 37°C.

PSB1C3+E0240+J23115 - PSB1C3+E0240+J23116 - PSB1C3+E0240+J23105:
Pre-culture from glycerol stock in LB+Cam (1:1000). Incubation 16h at 37°C.

Oct 08