"
3/13/14 Digested pBR322 with HindIII. Double digested pBR322 with HindIII and EcoRI. 3/27/14 Ran digests from 3/13/14 on gel. Excised gel fragments with plasmid in them. Purified gel fragments. 4/10/14 Phosphatase treated the digested and double digested pBR322 from 3/13/14. 4/17/14 Digested UV, HSP, and GFP g-blocks with HindIII and EcoRI. Performed five ligations: pBR322 with UV pBR322 with HSP pBR322 with GFP UV with GFP HSP with GFP 4/24/14 Purified UV+GFP and HSP+GFP ligations. Digested the two ligations with HindIII. Ligated each one into pBR322.
Team:Cooper Union/Notebook/Biohack April
From 2014.igem.org
(Difference between revisions)
(Created page with "<html> <link rel="stylesheet" href="https://2014.igem.org/Team:Cooper_Union/CSS?action=raw&ctype=text/css" type="text/css" /> <link rel="stylesheet" href="https://2014.igem.org/Tea...") |
|||
| Line 52: | Line 52: | ||
<div class="notebook-nav-container"> | <div class="notebook-nav-container"> | ||
<div class="month-links"> | <div class="month-links"> | ||
| - | <a href="https://2014.igem.org/Team:Cooper_Union/Notebook/Biohack_April">PRE MAY</a> | + | <a href="https://2014.igem.org/Team:Cooper_Union/Notebook/Biohack_April" class="selected">PRE MAY</a> |
| - | <a href="https://2014.igem.org/Team:Cooper_Union/Notebook/Biohack_May | + | <a href="https://2014.igem.org/Team:Cooper_Union/Notebook/Biohack_May">MAY</a> |
<a href="https://2014.igem.org/Team:Cooper_Union/Notebook/Biohack_June">JUN</a> | <a href="https://2014.igem.org/Team:Cooper_Union/Notebook/Biohack_June">JUN</a> | ||
<a href="https://2014.igem.org/Team:Cooper_Union/Notebook/Biohack_July">JUL</a> | <a href="https://2014.igem.org/Team:Cooper_Union/Notebook/Biohack_July">JUL</a> | ||
Revision as of 06:17, 16 October 2014
