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Team:BostonU/Data
From 2014.igem.org
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| - | <p><img src="https://static.igem.org/mediawiki/2014/b/b0/PTet_pBad_RFP_all_three.png" height=" | + | <p>For the first tandem promoter flow cytometry experiment, we tested pBad-pTet-BCD2-E1010m-B0015 and pTet-pBad-BCD2-E1010m-B0015. We planned on inducing each promoter separately with it's corresponding small molecule (either arabinose or atc) and also planned on inducing both together. We followed the <a href= "https://2014.igem.org/Team:BostonU/Protocols:>flow cytometry</a> workflow. By growing the constructs in different concentrations of media, we hoped to see RFP fluorescence increase as the small molecule concentration increased. For each concentration, we also had a negative. We also ran controls including: J04B2RM, J04B2GM, COXGR, COXRG, and DH5alpha. </p><img src="https://static.igem.org/mediawiki/2014/b/b0/PTet_pBad_RFP_all_three.png" height="500" width="500" style="float:right" style= "margin-left:10px"></p><br><br> |
<p><center><img src="https://static.igem.org/mediawiki/2014/3/39/PBad_pTet_RFP_all_three.png" width="35%"></center></p><br><br> | <p><center><img src="https://static.igem.org/mediawiki/2014/3/39/PBad_pTet_RFP_all_three.png" width="35%"></center></p><br><br> | ||
<p><center><img src="https://static.igem.org/mediawiki/2014/3/34/Origins_graph.png" width="50%"></center></p><br><br> | <p><center><img src="https://static.igem.org/mediawiki/2014/3/34/Origins_graph.png" width="50%"></center></p><br><br> | ||
Revision as of 02:14, 16 October 2014
Primer Design for Tandem Promoters and Repressor Genes
| Device Name | Forward Primer | Sequence | Reverse Primer | Sequence |
|---|---|---|---|---|
| BetI_CD | BetI_For_C | ATGAAGACGTAATGGTGCCGAAACTGGGTATGCAGAGC | BetI_Rev_D | ACGAAGACCTACCTTTAATCGGTCGGCAGATGCTGGGT |
| PhlF_CD | PhlF_For_C | ATGAAGACGTAATGATGGCACGTACCCCGAGCCGTAGC | PhlF_Rev_D | ACGAAGACCTACCTTTAACGCTGTGTACCCGGACAAAC |
| BM3R1_CD | BM3R1_For_C | ATGAAGACGTAATGATGGAAAGCACCCCGACCAAACAG | BM3R1_Rev_D | ACGAAGACCTACCTTTAGCTCTGACGGCTCAGTGCTGC |
| LmrA_CD | LmrA_For_C | ATGAAGACGTAATGATGAGCTATGGTGATAGCCGTGAA | LmrA_Rev_D | ACGAAGACCTACCTTTAACGTTTCAGCAGATCCGGAAT |
| SrpR_CD | SrpR_For_C | ATGAAGACGTAATGATGGCACGTAAAACCGCAGCAGAA | SrpR_Rev_D | ACGAAGACCTACCTTTATTCGAAGGATTTCACCTGTTT |
| pTet_AK | pTet_For_A | ATGAAGACGTGGAGTCCCTATCAGTGATAGAGATTGAC | pTet_Rev_K | ACGAAGACCTGCATTTCGGTCAGTGCGTCCTGCTGATG |
| pTet_KB | pTet_For_K | ATGAAGACGTATGCTCCCTATCAGTGATAGAGATTGAC | pTet_Rev_B | ACGAAGACCTAGTATTCGGTCAGTGCGTCCTGCTGATG |
| pBad_AK | pBad_For_A | ATGAAGACGTGGAGAAGAAACCAATTGTCCATATTGCA | pBad_Rev_K | ACGAAGACCTGCATTATGGAGAAACAGTAGAGAGTTGC |
| pBad_KB | pBad_For_K | ATGAAGACGTATGCAAGAAACCAATTGTCCATATTGCA | pBad_Rev_B | ACGAAGACCTAGTATATGGAGAAACAGTAGAGAGTTGC |
| pSrpR_KB | pSrpR_For_K | ATGAAGACGTATGCTTCGTTACCAATTGACAGCTAGCT | pSrpR_Rev_B | ACGAAGACCTAGTAGTTTACAAACAAACAAGCATGTAT |
| pLmrA_FK | pLmrA_For_F | ATGAAGACGTCGCTTTCGTTACCAATTGACAACTGGTG | pLmrA_Rev_K | ACGAAGACCTGCATAAATATAGTGACTGGTCTATTATC |
| pBetI_EB | pBet_For_E | ATGAAGACGTGCTTTTCATGGATTCGTTACCAATTGAC | pBetI_Rev_B | ACGAAGACCTAGTAGCTAGCATTATATTGAACGTCCAA |
| pPhlF_GB | pPhlF_For_G | ATGAAGACGTTGCCTTCGTTACCAATTGACATGATACG | pPhlF_Rev_B | ACGAAGACCTAGTAACCTTAACGATACGGTACGTTTCG |
| pBM3R1_FB | pBM3R1_For_F | ATGAAGACGTCGCTTTCGTTACCAATTGACGGAATGAA | pBM3R1_Rev_B | ACGAAGACCTAGTAGCTAGCATTATCGGAATGAACGTT |
Primer Design for Fusion Proteins
| Device Name | Primer | Sequence |
|---|---|---|
| C0080_CI | C0080_Rev_I | ACGAAGACCTTAGACAACTTGACGGCTACATCATTCAC |
| C0040_CI | C0040_Rev_I | ACGAAGACCTTAGACAACTTGACGGCTACATCATTCAC |
| E0040m_ID | E0040m_For_I | ATGAAGACGTTCTAGAATGCGTAAAGGAGAAGAACTTTTC |
| E0030_ID | E0030_For_I | ATGAAGACGTTCTAGAATGGTGAGCAAGGGCGAGGAGCTG |
Flow Cytometry Data
