Team:BostonU/Data

From 2014.igem.org

(Difference between revisions)
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     <td style="border:1px solid black;">pBad_Rev_B</td>
     <td style="border:1px solid black;">pBad_Rev_B</td>
     <td style="border:1px solid black;">ACGAAGACCTAGTATATGGAGAAACAGTAGAGAGTTGC</td>
     <td style="border:1px solid black;">ACGAAGACCTAGTATATGGAGAAACAGTAGAGAGTTGC</td>
 +
  </tr>
 +
<tr>
 +
    <td style="border:1px solid black;">pSrpR_KB</td>
 +
    <td style="border:1px solid black;">pSrpR_For_K </td>
 +
    <td style="border:1px solid black;">ATGAAGACGTATGCTTCGTTACCAATTGACAGCTAGCT</td>
 +
    <td style="border:1px solid black;">pSrpR_Rev_B</td>
 +
    <td style="border:1px solid black;">ACGAAGACCTAGTAGTTTACAAACAAACAAGCATGTAT</td>
 +
  </tr>
 +
<tr>
 +
    <td style="border:1px solid black;">pLmrA_FK</td>
 +
    <td style="border:1px solid black;">pLmrA_For_F </td>
 +
    <td style="border:1px solid black;">ATGAAGACGTCGCTTTCGTTACCAATTGACAACTGGTG</td>
 +
    <td style="border:1px solid black;">pLmrA_Rev_K</td>
 +
    <td style="border:1px solid black;">ACGAAGACCTGCATAAATATAGTGACTGGTCTATTATC</td>
 +
  </tr>
 +
<tr>
 +
    <td style="border:1px solid black;">pBetI_EB</td>
 +
    <td style="border:1px solid black;">pBet_For_E </td>
 +
    <td style="border:1px solid black;">ATGAAGACGTGCTTTTCATGGATTCGTTACCAATTGAC</td>
 +
    <td style="border:1px solid black;">pBetI_Rev_B</td>
 +
    <td style="border:1px solid black;">ACGAAGACCTAGTAGCTAGCATTATATTGAACGTCCAA</td>
 +
  </tr>
 +
<tr>
 +
    <td style="border:1px solid black;">pPhlF_GB</td>
 +
    <td style="border:1px solid black;">pPhlF_For_G </td>
 +
    <td style="border:1px solid black;">ATGAAGACGTTGCCTTCGTTACCAATTGACATGATACG</td>
 +
    <td style="border:1px solid black;">pPhlF_Rev_B</td>
 +
    <td style="border:1px solid black;">ACGAAGACCTAGTAACCTTAACGATACGGTACGTTTCG</td>
 +
  </tr>
 +
<tr>
 +
    <td style="border:1px solid black;">pBM3R1_FB</td>
 +
    <td style="border:1px solid black;">pBM3R1_For_F </td>
 +
    <td style="border:1px solid black;">ATGAAGACGTCGCTTTCGTTACCAATTGACGGAATGAA</td>
 +
    <td style="border:1px solid black;">pBM3R1_Rev_B</td>
 +
    <td style="border:1px solid black;">ACGAAGACCTAGTAGCTAGCATTATCGGAATGAACGTT</td>
   </tr>
   </tr>
</table></center>
</table></center>

Revision as of 01:57, 16 October 2014



Data Collected

Primer Design for Tandem Promoters and Repressor Genes

Device Name Forward Primer Sequence Reverse Primer Sequence
BetI_CD BetI_For_C ATGAAGACGTAATGGTGCCGAAACTGGGTATGCAGAGC BetI_Rev_D ACGAAGACCTACCTTTAATCGGTCGGCAGATGCTGGGT
PhlF_CD PhlF_For_C ATGAAGACGTAATGATGGCACGTACCCCGAGCCGTAGC PhlF_Rev_D ACGAAGACCTACCTTTAACGCTGTGTACCCGGACAAAC
BM3R1_CD BM3R1_For_C ATGAAGACGTAATGATGGAAAGCACCCCGACCAAACAG BM3R1_Rev_D ACGAAGACCTACCTTTAGCTCTGACGGCTCAGTGCTGC
LmrA_CD LmrA_For_C ATGAAGACGTAATGATGAGCTATGGTGATAGCCGTGAA LmrA_Rev_D ACGAAGACCTACCTTTAACGTTTCAGCAGATCCGGAAT
SrpR_CD SrpR_For_C ATGAAGACGTAATGATGGCACGTAAAACCGCAGCAGAA SrpR_Rev_D ACGAAGACCTACCTTTATTCGAAGGATTTCACCTGTTT
pTet_AK pTet_For_A ATGAAGACGTGGAGTCCCTATCAGTGATAGAGATTGAC pTet_Rev_K ACGAAGACCTGCATTTCGGTCAGTGCGTCCTGCTGATG
pTet_KB pTet_For_K ATGAAGACGTATGCTCCCTATCAGTGATAGAGATTGAC pTet_Rev_B ACGAAGACCTAGTATTCGGTCAGTGCGTCCTGCTGATG
pBad_AK pBad_For_A ATGAAGACGTGGAGAAGAAACCAATTGTCCATATTGCA pBad_Rev_K ACGAAGACCTGCATTATGGAGAAACAGTAGAGAGTTGC
pBad_KB pBad_For_K ATGAAGACGTATGCAAGAAACCAATTGTCCATATTGCA pBad_Rev_B ACGAAGACCTAGTATATGGAGAAACAGTAGAGAGTTGC
pSrpR_KB pSrpR_For_K ATGAAGACGTATGCTTCGTTACCAATTGACAGCTAGCT pSrpR_Rev_B ACGAAGACCTAGTAGTTTACAAACAAACAAGCATGTAT
pLmrA_FK pLmrA_For_F ATGAAGACGTCGCTTTCGTTACCAATTGACAACTGGTG pLmrA_Rev_K ACGAAGACCTGCATAAATATAGTGACTGGTCTATTATC
pBetI_EB pBet_For_E ATGAAGACGTGCTTTTCATGGATTCGTTACCAATTGAC pBetI_Rev_B ACGAAGACCTAGTAGCTAGCATTATATTGAACGTCCAA
pPhlF_GB pPhlF_For_G ATGAAGACGTTGCCTTCGTTACCAATTGACATGATACG pPhlF_Rev_B ACGAAGACCTAGTAACCTTAACGATACGGTACGTTTCG
pBM3R1_FB pBM3R1_For_F ATGAAGACGTCGCTTTCGTTACCAATTGACGGAATGAA pBM3R1_Rev_B ACGAAGACCTAGTAGCTAGCATTATCGGAATGAACGTT

Primer Design for Fusion Proteins

Device Name Primer Sequence
C0080_CI C0080_Rev_I ACGAAGACCTTAGACAACTTGACGGCTACATCATTCAC
C0040_CI C0040_Rev_I ACGAAGACCTTAGACAACTTGACGGCTACATCATTCAC
E0040m_ID E0040m_For_I ATGAAGACGTTCTAGAATGCGTAAAGGAGAAGAACTTTTC
E0030_ID E0030_For_I ATGAAGACGTTCTAGAATGGTGAGCAAGGGCGAGGAGCTG

















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