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Team:BostonU/Data
From 2014.igem.org
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<th style="border:1px solid black"><font color="#B22222">Device Name</font></th> | <th style="border:1px solid black"><font color="#B22222">Device Name</font></th> | ||
Revision as of 01:28, 16 October 2014
Primer Design for Tandem Promoters and Repressor Genes
| Device Name | Forward Primer | Sequence | Reverse Primer | Sequence |
|---|---|---|---|---|
| BetI_CD | BetI_For_C | ATGAAGACGTAATGGTGCCGAAACTGGGTATGCAGAGC | BetI_Rev_D | ACGAAGACCTACCTTTAATCGGTCGGCAGATGCTGGGT |
| PhlF_CD | PhlF_For_C | ATGAAGACGTAATGATGGCACGTACCCCGAGCCGTAGC | PhlF_Rev_D | ACGAAGACCTACCTTTAACGCTGTGTACCCGGACAAAC |
| BM3R1_CD | BM3R1_For_C | ATGAAGACGTAATGATGGAAAGCACCCCGACCAAACAG | BM3R1_Rev_D | ACGAAGACCTACCTTTAGCTCTGACGGCTCAGTGCTGC |
| LmrA_CD | LmrA_For_C | ATGAAGACGTAATGATGAGCTATGGTGATAGCCGTGAA | LmrA_Rev_D | ACGAAGACCTACCTTTAACGTTTCAGCAGATCCGGAAT |
| SrpR_CD | SrpR_For_C | ATGAAGACGTAATGATGGCACGTAAAACCGCAGCAGAA | SrpR_Rev_D | ACGAAGACCTACCTTTATTCGAAGGATTTCACCTGTTT |
| pTet_AK | pTet_For_A | ATGAAGACGTGGAGTCCCTATCAGTGATAGAGATTGAC | pTet_Rev_K | ACGAAGACCTGCATTTCGGTCAGTGCGTCCTGCTGATG |
| pTet_KB | pTet_For_K | ATGAAGACGTATGCTCCCTATCAGTGATAGAGATTGAC | pTet_Rev_B | ACGAAGACCTAGTATTCGGTCAGTGCGTCCTGCTGATG |
| pBad_AK | pBad_For_A | ATGAAGACGTGGAGAAGAAACCAATTGTCCATATTGCA | pBad_Rev_K | ACGAAGACCTGCATTATGGAGAAACAGTAGAGAGTTGC |
| pBad_KB | pBad_For_K | ATGAAGACGTATGCAAGAAACCAATTGTCCATATTGCA | pBad_Rev_B | ACGAAGACCTAGTATATGGAGAAACAGTAGAGAGTTGC |
Primer Design for Fusion Proteins
| Device Name | Primer | Sequence |
|---|---|---|
| C0080_CI | C0080_Rev_I | ACGAAGACCTTAGACAACTTGACGGCTACATCATTCAC |
| C0040_CI | C0040_Rev_I | ACGAAGACCTTAGACAACTTGACGGCTACATCATTCAC |
| E0040m_ID | E0040m_For_I | ATGAAGACGTTCTAGAATGCGTAAAGGAGAAGAACTTTTC |
| E0030_ID | E0030_For_I | ATGAAGACGTTCTAGAATGGTGAGCAAGGGCGAGGAGCTG |
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