Team:BostonU/Data

From 2014.igem.org

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<h2>Tandem Promoter and Repressor Gene Primers</h2>
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<h2>Primer Design for Tandem Promoters and Repressor Genes</h2>
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<h2>Primer Design for Fusion Proteins</h2>
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<table id="devices" style="border:1px solid black; border-collapse:collapse">
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    <th style="border:1px solid black"><font color="#B22222">Device Name</font></th>
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    <th style="border:1px solid black"><font color="#B22222">Primer</font></th>
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    <th style="border:1px solid black"><font color="#B22222">Sequence</font></th>
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C0080_Rev_I ACGAAGACCTTAGAcaacttgacggctacatcattcac
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C0040_Rev_I ACGAAGACCTTAGAagctactaaagcgtagttttcgtc
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E1010_For_I ATGAAGACGTTCTAGAatggcttcctccgaGgaTgttatc
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E0040_For_I ATGAAGACGTTCTAGAatgcgtaaaggagaagaacttttc
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E0030_For_I ATGAAGACGTTCTAGAatggtgagcaagggcgaggagctg
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    <td style="border:1px solid black;">C0080_CI</td>
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    <td style="border:1px solid black;">C0080_Rev_I</td>
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    <td style="border:1px solid black;"> ACGAAGACCTTAGACAACTTGACGGCTACATCATTCAC </td>
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    <td style="border:1px solid black;">C0040_CI</td>
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    <td style="border:1px solid black;">C0040_Rev_I</td>
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    <td style="border:1px solid black;"> ACGAAGACCTTAGACAACTTGACGGCTACATCATTCAC </td>
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    <td style="border:1px solid black;">E0040m_ID</td>
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    <td style="border:1px solid black;">E0040m_For_I</td>
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    <td style="border:1px solid black;"> ATGAAGACGTTCTAGAATGCGTAAAGGAGAAGAACTTTTC </td>
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    <td style="border:1px solid black;">E0030_ID</td>
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    <td style="border:1px solid black;">E0030_For_I</td>
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    <td style="border:1px solid black;"> ATGAAGACGTTCTAGAATGGTGAGCAAGGGCGAGGAGCTG </td>
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Revision as of 00:00, 16 October 2014



Data Collected

Primer Design for Tandem Promoters and Repressor Genes

Device Name Forward Primer Sequence Reverse Primer Sequence
BetI_CD BetI_For_C ATGAAGACGTAATGGTGCCGAAACTGGGTATGCAGAGC BetI_Rev_D ACGAAGACCTACCTTTAATCGGTCGGCAGATGCTGGGT
PhlF_CD PhlF_For_C ATGAAGACGTAATGATGGCACGTACCCCGAGCCGTAGC PhlF_Rev_D ACGAAGACCTACCTTTAACGCTGTGTACCCGGACAAAC
BM3R1_CD BM3R1_For_C ATGAAGACGTAATGATGGAAAGCACCCCGACCAAACAG BM3R1_Rev_D ACGAAGACCTACCTTTAGCTCTGACGGCTCAGTGCTGC
LmrA_CD LmrA_For_C ATGAAGACGTAATGATGAGCTATGGTGATAGCCGTGAA LmrA_Rev_D ACGAAGACCTACCTTTAACGTTTCAGCAGATCCGGAAT
SrpR_CD SrpR_For_C ATGAAGACGTAATGATGGCACGTAAAACCGCAGCAGAA SrpR_Rev_D ACGAAGACCTACCTTTATTCGAAGGATTTCACCTGTTT
pTet_AK pTet_For_A ATGAAGACGTGGAGTCCCTATCAGTGATAGAGATTGAC pTet_Rev_K ACGAAGACCTGCATTTCGGTCAGTGCGTCCTGCTGATG
pTet_KB pTet_For_K ATGAAGACGTATGCTCCCTATCAGTGATAGAGATTGAC pTet_Rev_B ACGAAGACCTAGTATTCGGTCAGTGCGTCCTGCTGATG
pBad_AK pBad_For_A ATGAAGACGTGGAGAAGAAACCAATTGTCCATATTGCA pBad_Rev_K ACGAAGACCTGCATTATGGAGAAACAGTAGAGAGTTGC
pBad_KB pBad_For_K ATGAAGACGTATGCAAGAAACCAATTGTCCATATTGCA pBad_Rev_B ACGAAGACCTAGTATATGGAGAAACAGTAGAGAGTTGC

Primer Design for Fusion Proteins

C0080_Rev_I ACGAAGACCTTAGAcaacttgacggctacatcattcac C0040_Rev_I ACGAAGACCTTAGAagctactaaagcgtagttttcgtc E1010_For_I ATGAAGACGTTCTAGAatggcttcctccgaGgaTgttatc E0040_For_I ATGAAGACGTTCTAGAatgcgtaaaggagaagaacttttc E0030_For_I ATGAAGACGTTCTAGAatggtgagcaagggcgaggagctg
Device Name Primer Sequence
C0080_CI C0080_Rev_I ACGAAGACCTTAGACAACTTGACGGCTACATCATTCAC
C0040_CI C0040_Rev_I ACGAAGACCTTAGACAACTTGACGGCTACATCATTCAC
E0040m_ID E0040m_For_I ATGAAGACGTTCTAGAATGCGTAAAGGAGAAGAACTTTTC
E0030_ID E0030_For_I ATGAAGACGTTCTAGAATGGTGAGCAAGGGCGAGGAGCTG

















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