Team:UFAM Brazil/7-15-2014

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<b>Transformation Day =)</b>
<b>Transformation Day =)</b>
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<p> We made chemical competences with DH5a and we transformed it with:</p>
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<p> We made chemically competent cells with DH5α and we transformed it with:</p>
<p style="margin-left:50px">- Negative Control, no DNA;</p>
<p style="margin-left:50px">- Negative Control, no DNA;</p>
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<p style="margin-left:50px">- BBa_K346004 – pSB1C3 (Bioaccumulation Biobrick).</p>
<p style="margin-left:50px">- BBa_K346004 – pSB1C3 (Bioaccumulation Biobrick).</p>
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<p style="margin-top:20px">  We used chloramphenicol’s concentrations in the 34ug/ml plates.</p>
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<p style="margin-top:20px">  We used chloramphenicol’s concentrations in 34 ug/ml plates.</p>
<p> Studying hard to find a way to produce culture media with Mercury Chloride!!!!</p>
<p> Studying hard to find a way to produce culture media with Mercury Chloride!!!!</p>
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Latest revision as of 21:20, 15 October 2014

07/15/2014

Transformation Day =)

We made chemically competent cells with DH5α and we transformed it with:

- Negative Control, no DNA;

- Positive Control with pUC72;

- BBa_E0840 – pSB1C3 (Biodetection Biobrick);

- BBa_K346004 – pSB1C3 (Bioaccumulation Biobrick).

We used chloramphenicol’s concentrations in 34 ug/ml plates.

Studying hard to find a way to produce culture media with Mercury Chloride!!!!

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