From 2014.igem.org
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| - | {{:Team:Goettingen/header}}
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| - | {{:Team:Goettingen/proLP}}
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| - | <html><div class="proRP">
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| - | <h3 id="construction">Peptide Library Construction</h3>
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| - | <p>Starting with GFP scaffolds (Denoted pRS 316-GFPM), random loop libraries were created. Randomized amino acids were inserted at Asp-102/Asp-103 and Glu-172/Asp-173 by using the NNK method in order to create the random loop libraries. NNK sequences in primers number 13 and 16 were used to create regions I and ӀӀӀ. After having randomized DNA sequences, these two regions were assembled with region ӀӀ which remained intact. In each of the amplification and extension steps, <a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Pfus_PCR"><i>Pfus</i> PCR protocol</a> was used. For the assembly of the three regions, an <a href="https://2014.igem.org/Team:Goettingen/protocol_PCR#Asse_PCR">assembly PCR</a> was used. <br /><br />
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| - | </p>
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| - | <center><img src="https://static.igem.org/mediawiki/2014/d/d3/Goettingen_peptide_library.png" width="350px"/></center><br />
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| - | <p style="text-align:center"><b>Figure. Peptide Library Construction</b></p> <br /><br />
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| - | <div><a href="https://2014.igem.org/Team:Goettingen/protocol_Colony" class="button_pre"><b>Previous</b></a></div>
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| - | </div>
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| - | <div id="footerbanner"><a href="https://2014.igem.org/Team:Goettingen/team_sponsors"><img src="https://static.igem.org/mediawiki/2014/7/7a/Goettingen_footer_banner.png" /></a></div>
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| - | </html>
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Latest revision as of 21:18, 15 October 2014
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