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Team:UFAM Brazil/7-8-2014
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<tr><td colspan="3"><p> Unfortunately, we couldn’t transform the diluted DNA from the 2013 DNA kit. </p> | <tr><td colspan="3"><p> Unfortunately, we couldn’t transform the diluted DNA from the 2013 DNA kit. </p> | ||
| - | <p> Today ,instead of trying to transform using JM110 by washing it with sorbitol 1M or | + | <p> Today, instead of trying to transform using JM110 by washing it with sorbitol 1M or using a commercial cell, we used <i>Escherichia coli</i> DH5α chemically competent cell to transform biobrick BBa_E0840.</p> |
| - | <p> We also transformed Escherichia coli DH5α with pUC72 having approximately 1.000ng/ul and 1ng/ul, because we wanted to find out | + | <p> We also transformed <i>Escherichia coli</i> DH5α with pUC72 having approximately 1.000ng/ul and 1ng/ul, because we wanted to find out if the reason why the diluted DNA didn’t work was due to its low concentration.</p> |
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| - | <td class="back" ><a href="https://2014.igem.org/Team:UFAM_Brazil/7-7-2014"> | + | <td class="back" ><a href="https://2014.igem.org/Team:UFAM_Brazil/7-7-2014">Back</a></td> |
| - | <td class="next" ><a href="https://2014.igem.org/Team:UFAM_Brazil/7-9-2014">Next | + | <td class="next" ><a href="https://2014.igem.org/Team:UFAM_Brazil/7-9-2014">Next</a></td> |
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Latest revision as of 20:58, 15 October 2014
07/08/2014 | ||
Unfortunately, we couldn’t transform the diluted DNA from the 2013 DNA kit. Today, instead of trying to transform using JM110 by washing it with sorbitol 1M or using a commercial cell, we used Escherichia coli DH5α chemically competent cell to transform biobrick BBa_E0840. We also transformed Escherichia coli DH5α with pUC72 having approximately 1.000ng/ul and 1ng/ul, because we wanted to find out if the reason why the diluted DNA didn’t work was due to its low concentration. | ||
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