Team:Tianjin/Judging

From 2014.igem.org

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<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
 
-
<h1 >WELCOME TO iGEM 2014! </h1>
 
-
<p>Your team has been approved and you are ready to start the iGEM season!
 
-
<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
 
-
<br>
 
-
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Tianjin/Parts&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
 
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        <tr bgcolor="#3A2108">
 +
          <td height="50"><span class="STYLE69"><a href="#part1">Biobrick</a></span></td>
 +
        </tr>
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        <tr bgcolor="#3A2108">
 +
          <td height="50"><span class="STYLE69"><a href="#part2">Achievements</a></span></td>
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        </tr>
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        <tr bgcolor="#3A2108">
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          <td height="35" bgcolor="#663300"><span class="STYLE69"><a href="#top">Go to the top</a></span></td>
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</div> 
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<table width="1230" border="0" cellspacing="0" cellpadding="0">
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  <tr>
 +
    <td width="19%" bgcolor="#3A2108"><p><a href="https://2014.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2014/5/5d/Team：Tianjin_image_IGEM_official_logo.png" alt="image igem" width="205" height="114" border="0" align="top" /></a><br />
 +
    </p>
 +
        <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p></td>
 +
    <td width="43%" bgcolor="#3A2108"><p><span class="STYLE43"><span class="STYLE24"><a name="top" id="top"></a><span class="STYLE77">T</span></span><span class="STYLE76">rans<em>f</em> ibre</span></span><br />
 +
            <span class="STYLE40">Welcome to Team Tianjin! </span><br />
 +
    </p></td>
 +
    <td width="38%" bgcolor="#3A2108"><img src="https://static.igem.org/mediawiki/2014/e/eb/Tianjin-head3.gif" width="456" height="226" align="right" /></td>
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        <tr>
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          <td bgcolor="#663300"><span class="STYLE45"><a href="https://2014.igem.org/Team:Tianjin">Home|</a></span></td>
 +
        </tr>
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    <td width="169" bgcolor="#663300"><div class="STYLE46" id="project" onmouseover="MM_showHideLayers('Layer8','','show')" onmouseout="MM_showHideLayers('Layer8','','hide')"><a href="https://2014.igem.org/Team:Tianjin/Project" class="STYLE48">Project|</a></div></td>
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    <td width="174" bgcolor="#663300"><div id="judging" onmouseover="MM_showHideLayers('Layer11','','show')" onmouseout="MM_showHideLayers('Layer11','','hide')">
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 +
        <tr>
 +
          <td bgcolor="#663300"><a href="https://2014.igem.org/Team:Tianjin/Judging" class="STYLE47">Judging|</a></td>
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        <tr>
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          <td bgcolor="#663300"><a href="https://2014.igem.org/Team:Tianjin/Notebook" class="STYLE47">Notebook|</a></td>
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          <tr>
 +
            <td bgcolor="#663300"><span class="STYLE45"><a href="https://2014.igem.org/Team:Tianjin/Team">Team|</a></span></td>
 +
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    </div></td>
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          <tr>
 +
            <td bgcolor="#663300"><span class="STYLE45"><a href="https://2014.igem.org/Team:Tianjin/Safety">Safety|</a></span></td>
 +
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            <td bgcolor="#663300"><span class="STYLE45"><a href="https://2014.igem.org/Team:Tianjin/HumanPractice">Human&nbsp;Practice|</a></span></td>
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<br />
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<table width="1230" border="0" align="center" cellpadding="0" cellspacing="0">
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  <tr>
 +
    <td bgcolor="#993300"><table width="800" border="10" align="center" cellpadding="0" cellspacing="0" bordercolor="#C67B14" bgcolor="#993300">
 +
      <tr>
 +
        <td bgcolor="#C67B14"><span class="STYLE62"><a name="part1" id="part1"></a><strong>①Biobrick</strong></span></td>
 +
      </tr>
 +
      <tr>
 +
        <td width="100%" bgcolor="#FFED97"><p class="STYLE33"><strong>BBa_K1361000</strong></p>
 +
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            <tr>
 +
              <td bgcolor="#FFED97" class="STYLE33"><img src="https://static.igem.org/mediawiki/2014/f/f0/Tianjin-biobrick.png" width="500" height="60" /></td>
 +
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 +
          <p class="STYLE33">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Based on the concept that change of cell  motility by control of CheZ chemotaxis regulator could disturb pre-expressed  curli fiber structure, we assume that if the inducer occurs, in this case  acacia, CsgB protein will secrete into extracellular and act as nucleator to  recruit soluble CsgA monomer into amyloid fibres, and the transcription of CheZ  will be downgrade via cI-Plamda NOT gate. If induction condition disappears at  a certain point, suppression of CheZ will be discharged. As a result, cells  will be more moveable thus disturb or inhibit the establishment of curli  structure. And in our final device, the dynamic detection could achieve by  monitoring current change between two electrodes in the culture.</p>
 +
          <p class="STYLE33"><strong>BBa_K1361001</strong></p>
 +
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
 +
            <tr>
 +
              <td bgcolor="#FFED97" class="STYLE33"><img src="https://static.igem.org/mediawiki/2014/8/86/Tianjin-biobrick1.png" width="350" height="60" /></td>
 +
            </tr>
 +
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 +
          <p class="STYLE33">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This part contains the major subunit of  curli fiber CsgA and its nucleator CsgB, in which CsgA is constitutive  expression(a relatively weak promoter compared to BBa_K1361003) whereas CsgB is  under the control of T7 promotor. This part cannot functioning alone without  expression of CsgEFG genes in the same cell, for they coding the outer membrane  channel secrete system for CsgA and CsgB.</p>
 +
          <p class="STYLE33"><strong>BBa_K1361002</strong></p>
 +
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 +
            <tr>
 +
              <td height="61" bgcolor="#FFED97" class="STYLE33"><img src="https://static.igem.org/mediawiki/2014/3/37/Tianjin-biobrick2.png" width="350" height="60" /></td>
 +
            </tr>
 +
          </table>
 +
          <p class="STYLE33">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This part contains the major subunit of  curli fiber CsgA and its nucleator CsgBtrunc, in which CsgA is constitutive  expression whereas CsgBtrunc is under the control of Pbad promotor. CsgBtrunc  is different from native CsgB for the deletion of C-terminal amino acid from  133 to 155 so that it cannot attach cell outer membrane. And CsgBtrunc itself  is highly aggregative in the culture. This part is designed for immediate  generation of large amount of curli fiber after adding the inducer. This part  cannot function alone without the expression of CsgEFG genes in the same cell,  for they coding the outer membrane channel secrete system for CsgA and CsgB.</p>
 +
          <p class="STYLE33"><strong>BBa_K1361003</strong></p>
 +
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
 +
            <tr>
 +
              <td height="61" bgcolor="#FFED97" class="STYLE33"><img src="https://static.igem.org/mediawiki/2014/b/b1/Tianjin-biobrick3.png" width="350" height="60" /></td>
 +
            </tr>
 +
          </table>
 +
          <p class="STYLE33">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This part contains the major subunit of  curli fiber CsgA and its nucleator CsgB, in which CsgA is constitutive  expression(a relatively strong promoter compared to BBa_K1361001) whereas CsgB  is under the control of T7 promoter. This part cannot function alone without  expression of CsgEFG genes in the same cell, for they coding the outer membrane  channel secrete system for CsgA and CsgB.</p>
 +
          <p class="STYLE33"><strong>BBa_K1361004</strong></p>
 +
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
 +
            <tr>
 +
              <td height="61" bgcolor="#FFED97" class="STYLE33"><img src="https://static.igem.org/mediawiki/2014/3/31/Tianjin-biobrick4.png" width="350" height="60" /></td>
 +
            </tr>
 +
          </table>
 +
          <p class="STYLE33">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This part contains the major subunit of  curli fiber CsgA-his (CsgA modified by His tag) and its nucleator CsgB, in  which CsgA-his is constitutive expression whereas CsgB is under the control of  Pbad promoter. This part is designed for immediate generation of large amount  of curli fiber after the adding of the inducer and the purification of CsgA by Ni-NTA  reign or absorbing Au-NTA-Ni nano partial. This part cannot function alone  without expression of CsgEFG genes in the same cell, for they coding the outer  membrane channel secrete system for CsgA and CsgB.</p>
 +
          <p class="STYLE33"><strong>BBa_K1361005</strong></p>
 +
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
 +
            <tr>
 +
              <td height="61" bgcolor="#FFED97" class="STYLE33"><img src="https://static.igem.org/mediawiki/2014/b/b7/Tianjin-biobrick5.png" width="150" height="60" /></td>
 +
            </tr>
 +
          </table>
 +
          <p class="STYLE33">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This part contains CsgE, CsgF, CsgG, which  serve as the outer membrane secrete device for curli fiber, at relatively low  constitutive. This part is for specific transport of CsgA(BBa_K1361999) and CsgB(BBa_K1361997)  into extracellular matrix. CsgEFG(BBa_K1361992) was placed after a relatively  weak constitutive promoter.</p>
 +
          <p class="STYLE33"><strong>BBa_K1361006</strong></p>
 +
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
 +
            <tr>
 +
              <td height="61" bgcolor="#FFED97" class="STYLE33"><img src="https://static.igem.org/mediawiki/2014/f/fb/Tianjin-biobrick6.png" width="350" height="60" /></td>
 +
            </tr>
 +
          </table>
 +
          <p class="STYLE33">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This part contains the major subunit of  curli fiber CsgA and its nucleator CsgBtrunc (a dissociative nucleator), in  which CsgA is constitutive expression whereas CsgBtrunc is under the control of  Pbad promoter. CsgBtrunc is different from native CsgB for the deletion of  C-terminal amino acid from 133 to 155 so that it cannot attach cell outer  membrane. And CsgBtrunc itself is highly aggregative in the culture. This part  is designed for immediate generation of large amount of curli fiber after the adding  of the inducer, and a stronger promoter for CsgA was chosen to parallel with  BBa_K1361002. This part cannot function alone without expression of CsgEFG  genes in the same cell, for they coding the outer membrane channel secrete  system for CsgA and CsgB.</p>
 +
          <p class="STYLE33"><strong>BBa_K1361007</strong></p>
 +
          <table width="100%" border="0" cellspacing="0" cellpadding="0">
 +
            <tr>
 +
              <td height="61" bgcolor="#FFED97" class="STYLE33"><img src="https://static.igem.org/mediawiki/2014/d/d8/Tianjin-biobrick7.png" width="450" height="60" /></td>
 +
            </tr>
 +
          </table>
 +
          <p class="STYLE33">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;This part contains the major subunit of  curli fiber CsgA-his CsgA modified by His tag) and its  nucleator CsgB, in which CsgA-his is constitutive expression whereas CsgB is  under the control of Pbad promoter. This part is designed for immediate generation  of large amount of curli fiber after adding of the inducer and the purification  of CsgA by Ni-NTA reign, or absorbing Au-NTA-Ni nano partical. This part cannot  function alone without expression of CsgEFG genes in the same cell, for they  coding the outer membrane channel secrete system for CsgA and CsgB.</p>          </td>
 +
      </tr>
 +
      <tr>
 +
        <td height="56" bgcolor="#C67B14"><span class="STYLE29"><a name="part2" id="part2"></a><span class="STYLE62"><strong>②Achievements</strong></span></span></td>
 +
      </tr>
 +
      <tr>
 +
        <td bgcolor="#FFED97"><p><span class="STYLE33">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></p>
 +
          </td>
 +
      </tr>
 +
    </table></td>
 +
  </tr>
 +
</table>
 +
<br />
 +
<table width="1230" border="5" cellpadding="0" cellspacing="0" bordercolor="#993300" bgcolor="#FFFFFF">
 +
  <tr>
 +
    <td height="59" bgcolor="#993300"><table width="100%" border="15" cellpadding="0" cellspacing="0" bordercolor="#3A2108">
 +
      <tr>
 +
        <td width="19%" bgcolor="#3A2108"><a href="http://www.tju.edu.cn/index.htm"><img src="https://static.igem.org/mediawiki/2014/0/01/Tianjin-school.png" width="175" height="163" border="0" /></a></td>
 +
        <td width="81%" bgcolor="#3A2108"><p><span class="STYLE61">Our sponser:<br />
 +
        Tianjin University<br />
 +
          Eddress:
 +
          Tianjin University, Weijin street No.92, Tianjin, China<br />
 +
          Email:@edu.tju.cn</span></p>
 +
          <p>&nbsp;</p></td>
 +
      </tr>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
    </table>    
-
<a href="https://2014.igem.org/Team:Tianjin"style="color:#000000">Home </a> </td>
+
      <p class="STYLE18"><br />
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    </p>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
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    </td>
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<a href="https://2014.igem.org/Team:Tianjin/Team"style="color:#000000"> Team </a> </td>
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<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Tianjin"style="color:#000000"> Official Team Profile </a></td>
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<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
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<a href="https://2014.igem.org/Team:Tianjin/Project"style="color:#000000"> Project</a></td>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<a href="https://2014.igem.org/Team:Tianjin/Parts"style="color:#000000"> Parts</a></td>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<a href="https://2014.igem.org/Team:Tianjin/Modeling"style="color:#000000"> Modeling</a></td>
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<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
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<a href="https://2014.igem.org/Team:Tianjin/Notebook"style="color:#000000"> Notebook</a></td>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<a href="https://2014.igem.org/Team:Tianjin/Safety"style=" color:#000000"> Safety </a></td>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<a href="https://2014.igem.org/Team:Tianjin/Attributions"style="color:#000000"> Attributions </a></td>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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</table>
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      <script language="javascript">
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    var speed = 100;
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    var scrollTop = null;
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    var hold = 0;
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    scrollTop = document.documentElement.scrollTop+document.body.scrollTop;
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<tr> <td colspan="3"  height="15px"> </td></tr>
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    pos = pos/10
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    moveHeight = pos>0?Math.ceil(pos):Math.floor(pos);
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<!--Parts Submitted to the Registry  -->
+
    }
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<tr><td > <h3> Parts Submitted to the Registry </h3></td>
+
    //alert(scrollTop);
-
<td ></td >
+
    }
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<td > <h3>What information do I need to start putting my parts on the Registry? </h3></td>
+
    </script>
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<td width="45%"  valign="top">
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<p>
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will submit them to the <a href="http://partsregistry.org"> Registry of Standard Biological Parts</a>. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox. 
+
-
 
+
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<p>
+
-
<strong>Note that if you want to document a part you need to document it on the <a href="http://partsregistry.org Registry"> Registry</a>, not on your team wiki.</strong> Future teams and other users and are much more likely to find parts on the Registry than on your team wiki.
+
</p>
</p>
 +
</body>
-
<p>
 
-
Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
 
-
</p>
 
-
 
-
 
-
 
-
<h3>When should you put parts into the Registry?</h3>
 
-
 
-
<p>
 
-
As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements.
 
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-
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-
 
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<td > </td>
 
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<td width="45%" valign="top">
 
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<p>
 
-
The information needed to initially create a part on the Registry is:
 
-
</p>
 
-
<ol>
 
-
 
-
<li>Part Name</li>
 
-
<li>Part type</li>
 
-
<li>Creator</li>
 
-
<li>Sequence</li>
 
-
<li>Short Description (60 characters on what the DNA does)</li>
 
-
<li>Long Description (Longer description of what the DNA does)</li>
 
-
<li>Design considerations</li>
 
-
</ol>
 
-
 
-
<p>
 
-
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part <a href="http://parts.igem.org/Part:BBa_K404003">BBa_K404003</a> for an excellent example of a highly characterized part.
 
-
</p>
 
-
 
-
<p>
 
-
You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry"> Add a Part to the Registry</a> link.
 
-
</p>
 
-
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-
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-
 
-
 
-
<tr> <td colspan="3"  height="15px"> </td></tr>
 
-
 
-
<tr><td colspan="3" > <h3> Parts Table</h3></td></tr>
 
-
 
-
 
-
<tr><td width="45%" colspan="3"  valign="top">
 
-
Any parts your team has created will appear in this table below:</td></tr>
 
-
 
-
</table>
 
</html>
</html>
-
 
-
<groupparts>iGEM013 Tianjin</groupparts>
 

Revision as of 20:46, 15 October 2014

Team:Tianjin2014/Safety

Biobrick
Achievements
Go to the top

image igem

 

 

 

Transf ibre
Welcome to Team Tianjin!


①Biobrick

BBa_K1361000

        Based on the concept that change of cell motility by control of CheZ chemotaxis regulator could disturb pre-expressed curli fiber structure, we assume that if the inducer occurs, in this case acacia, CsgB protein will secrete into extracellular and act as nucleator to recruit soluble CsgA monomer into amyloid fibres, and the transcription of CheZ will be downgrade via cI-Plamda NOT gate. If induction condition disappears at a certain point, suppression of CheZ will be discharged. As a result, cells will be more moveable thus disturb or inhibit the establishment of curli structure. And in our final device, the dynamic detection could achieve by monitoring current change between two electrodes in the culture.

BBa_K1361001

        This part contains the major subunit of curli fiber CsgA and its nucleator CsgB, in which CsgA is constitutive expression(a relatively weak promoter compared to BBa_K1361003) whereas CsgB is under the control of T7 promotor. This part cannot functioning alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.

BBa_K1361002

        This part contains the major subunit of curli fiber CsgA and its nucleator CsgBtrunc, in which CsgA is constitutive expression whereas CsgBtrunc is under the control of Pbad promotor. CsgBtrunc is different from native CsgB for the deletion of C-terminal amino acid from 133 to 155 so that it cannot attach cell outer membrane. And CsgBtrunc itself is highly aggregative in the culture. This part is designed for immediate generation of large amount of curli fiber after adding the inducer. This part cannot function alone without the expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.

BBa_K1361003

        This part contains the major subunit of curli fiber CsgA and its nucleator CsgB, in which CsgA is constitutive expression(a relatively strong promoter compared to BBa_K1361001) whereas CsgB is under the control of T7 promoter. This part cannot function alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.

BBa_K1361004

        This part contains the major subunit of curli fiber CsgA-his (CsgA modified by His tag) and its nucleator CsgB, in which CsgA-his is constitutive expression whereas CsgB is under the control of Pbad promoter. This part is designed for immediate generation of large amount of curli fiber after the adding of the inducer and the purification of CsgA by Ni-NTA reign or absorbing Au-NTA-Ni nano partial. This part cannot function alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.

BBa_K1361005

        This part contains CsgE, CsgF, CsgG, which serve as the outer membrane secrete device for curli fiber, at relatively low constitutive. This part is for specific transport of CsgA(BBa_K1361999) and CsgB(BBa_K1361997) into extracellular matrix. CsgEFG(BBa_K1361992) was placed after a relatively weak constitutive promoter.

BBa_K1361006

        This part contains the major subunit of curli fiber CsgA and its nucleator CsgBtrunc (a dissociative nucleator), in which CsgA is constitutive expression whereas CsgBtrunc is under the control of Pbad promoter. CsgBtrunc is different from native CsgB for the deletion of C-terminal amino acid from 133 to 155 so that it cannot attach cell outer membrane. And CsgBtrunc itself is highly aggregative in the culture. This part is designed for immediate generation of large amount of curli fiber after the adding of the inducer, and a stronger promoter for CsgA was chosen to parallel with BBa_K1361002. This part cannot function alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.

BBa_K1361007

        This part contains the major subunit of curli fiber CsgA-his CsgA modified by His tag) and its nucleator CsgB, in which CsgA-his is constitutive expression whereas CsgB is under the control of Pbad promoter. This part is designed for immediate generation of large amount of curli fiber after adding of the inducer and the purification of CsgA by Ni-NTA reign, or absorbing Au-NTA-Ni nano partical. This part cannot function alone without expression of CsgEFG genes in the same cell, for they coding the outer membrane channel secrete system for CsgA and CsgB.
②Achievements

       

Our sponser:
Tianjin University
Eddress: Tianjin University, Weijin street No.92, Tianjin, China
Email:@edu.tju.cn

 


Retrieved from "http://2014.igem.org/Team:Tianjin/Judging"