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Team:UCLA/Notebook/Protocols
From 2014.igem.org
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<a href="https://static.igem.org/mediawiki/2014/d/d2/Silk_Fibroin_Extraction_%26_Film_Formation_Protocol%283%29.pdf">Electrospun Fiber Protocol</a></td></tr> | <a href="https://static.igem.org/mediawiki/2014/d/d2/Silk_Fibroin_Extraction_%26_Film_Formation_Protocol%283%29.pdf">Electrospun Fiber Protocol</a></td></tr> | ||
| - | + | <td></br></td> | |
| - | <tr><td><br></br></td></tr> | + | <tr><td><b>Silk Thread</b></br> |
| + | <a href="https://static.igem.org/mediawiki/2014/6/6e/Silk_Thread_Protocols.pdf">Silk Thread Protocol</a> | ||
| + | <br>Outline</br> | ||
| + | <ol> | ||
| + | <li>Construction of the Synthetic Spider Silk Multimeric Gene</li> | ||
| + | <li>Cloning of the spider silk multimer into the expression vector</li> | ||
| + | <li>Gene Expression of pETsilk</li> | ||
| + | <li>E.coli Cell Lysis</li> | ||
| + | <li>Silk Protein Purification Using IMAC</li> | ||
| + | <li>Dialysis</li> | ||
| + | <li>Protein Analysis</li> | ||
| + | <li>Amino Acid Analysis</li> | ||
| + | <li>Overall: Timing of Procedure</li> | ||
| + | </ol></td></tr> | ||
| + | <tr><td></br></td></tr> | ||
<tr><td><h3> Reference </h3><b>Materials Fabrication</b></br>Rockwood, Danielle N., Rucsanda C. Preda, Tuna Yücel, Xiaoqin Wang, Michael L. Lovett, and David L. Kaplan. "Materials Fabrication from Bombyx Mori Silk Fibroin." Nature Protocols 6.10 (2011): 1612-631. Web. <br></br> | <tr><td><h3> Reference </h3><b>Materials Fabrication</b></br>Rockwood, Danielle N., Rucsanda C. Preda, Tuna Yücel, Xiaoqin Wang, Michael L. Lovett, and David L. Kaplan. "Materials Fabrication from Bombyx Mori Silk Fibroin." Nature Protocols 6.10 (2011): 1612-631. Web. <br></br> | ||
<br><b>Production of MASP1/2</b></br>Teulé, Florence, Alyssa R. Cooper, William A. Furin, Daniela Bittencourt, Elibio L. Rech, Amanda Brooks, and Randolph V. Lewis. "A Protocol for the Production of Recombinant Spider Silk-like Proteins for Artificial Fiber Spinning." Nature Protocols 4.3 (2009): 341-55. Web.</br></td></tr> | <br><b>Production of MASP1/2</b></br>Teulé, Florence, Alyssa R. Cooper, William A. Furin, Daniela Bittencourt, Elibio L. Rech, Amanda Brooks, and Randolph V. Lewis. "A Protocol for the Production of Recombinant Spider Silk-like Proteins for Artificial Fiber Spinning." Nature Protocols 4.3 (2009): 341-55. Web.</br></td></tr> | ||
| - | <tr><td></ | + | <tr><td></br></br></br></td></tr> |
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</table> | </table> | ||
</html> | </html> | ||
Revision as of 22:40, 2 July 2014
Protocols | ||
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You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well. |
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Equipment for the collection of recombinant silkEquipment Protocol | ||
List of Reagents Reagent Protocol | ||
PCR for MASP1 and MASP2 PCR Protocol | ||
Silk Fibroin ExtractionSilk Fibroin Extraction Protocol | ||
Silk Films Silk Films Protocol | ||
Electrospun Fibers Electrospun Fiber Protocol | ||
| Silk Thread
Silk Thread Protocol
Outline
| ||
ReferenceMaterials FabricationRockwood, Danielle N., Rucsanda C. Preda, Tuna Yücel, Xiaoqin Wang, Michael L. Lovett, and David L. Kaplan. "Materials Fabrication from Bombyx Mori Silk Fibroin." Nature Protocols 6.10 (2011): 1612-631. Web.Production of MASP1/2Teulé, Florence, Alyssa R. Cooper, William A. Furin, Daniela Bittencourt, Elibio L. Rech, Amanda Brooks, and Randolph V. Lewis. "A Protocol for the Production of Recombinant Spider Silk-like Proteins for Artificial Fiber Spinning." Nature Protocols 4.3 (2009): 341-55. Web. | ||
