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Team:BIT/achievement.html
From 2014.igem.org
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We constructed an amplification plasmid,containg RhlR protein(LVA-) and RhlR/PAI2 Inducible promoter with RFP. Then we transfected the plamid to Trans 5α cells, and used the cells to do follow experiments. | We constructed an amplification plasmid,containg RhlR protein(LVA-) and RhlR/PAI2 Inducible promoter with RFP. Then we transfected the plamid to Trans 5α cells, and used the cells to do follow experiments. | ||
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Revision as of 19:30, 15 October 2014
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constitutive promoter+RhlR protein generator(LVA-)+RhlR/PAI2 Inducible promoter+RBS(B0030)+RFP+Terminator The max excitation wavelength for the RFP we used is 584nm,and the max emission wavelength is 607nm. We constructed an amplification plasmid,containg RhlR protein(LVA-) and RhlR/PAI2 Inducible promoter with RFP. Then we transfected the plamid to Trans 5α cells, and used the cells to do follow experiments.
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