Team:AMU-Poznan/Modeling
From 2014.igem.org
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+ | Different algorithms gives very different results. To have the most reliable structure it recommended to check the structure</br> | ||
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Revision as of 18:03, 15 October 2014
Survey14.10.2014 31 survey responses: what survey answerers think that RNAi is ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ConclusionsKnowledge of RNAi is good. however people do not know what exactly sh-miR is. Users are eager to wait up to 8h, 24h only 50% and 2 days only 20% (work without software will take 2 days). What are the most important features of bioinformatic software: user-friendly interface documentation easy user-guide fast response from software what is important in shmiR designer: speed of software possibilty to choose miRNA scaffold experimental evaluationModelingWe decided to pay more attention to model sh-miR molecules with different RNA 2D structure prediction software (we use RNAfold in our software). Here are our results: analyzed sequence: CTAAAGAAGGTATATTGCTGTTGACAGTGAGCGACTGUUUGUAUUCGCCCUAGCGCCTGTGAAGCCACAGATGGGGCGCUAUGGCGAAUACAAACATGCCTACTGCCTCGGACTTCAAGGGGCTACTTTAGGAGCA We always take first generated structure made with default options If there is difference in algoruthm we always take RNA folding over DNA folding mfold![]() ![]() ![]() ConclusionsDifferent algorithms gives very different results. To have the most reliable structure it recommended to check the structure with different software. |