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Team:Valencia UPV/VUPV Parts
From 2014.igem.org
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<p>Four of them could be sent thanks to the collaboration from the <a class="normal-link-page" href="https://2014.igem.org/Team:NRP-UEA-Norwich">NRP-UEA-Norwich</a> team, who very kindly provided us with their MoFlippers. These were the parts coding sequences for <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554001">AtrΔ11</a>, <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554002">HarFAR</a> and <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554003">EaDAcT</a> and the <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554000">TA29</a> promoter.</p><br/> | <p>Four of them could be sent thanks to the collaboration from the <a class="normal-link-page" href="https://2014.igem.org/Team:NRP-UEA-Norwich">NRP-UEA-Norwich</a> team, who very kindly provided us with their MoFlippers. These were the parts coding sequences for <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554001">AtrΔ11</a>, <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554002">HarFAR</a> and <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554003">EaDAcT</a> and the <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554000">TA29</a> promoter.</p><br/> | ||
| - | <p>We designed | + | <p>We also designed a vector that has de backbone of the Biobrick vector pSB1A3 but compatible with GoldenBraid 2.0. It contains two BsmBI restriction sites which leave the required overhangs to perform a GoldenBraid ligation of two transcriptional units in alpha vectors. We call it <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554006">"omega undercover"</a> as it works in a similar way of the GoldenBraid 2.0 omega vector but with a pSB1A3 backbone.</p><br/> |
<div align="center"><img src="https://static.igem.org/mediawiki/2014/4/45/VUPVomega_undercover.jpg" alt="omega_undercover"></img></div><br/> | <div align="center"><img src="https://static.igem.org/mediawiki/2014/4/45/VUPVomega_undercover.jpg" alt="omega_undercover"></img></div><br/> | ||
| - | <p>Using the omega undercover plasmid we constructed the two biosafety devices which | + | <p>Using the omega undercover plasmid, we constructed the two biosafety devices which consist on an identity preservation module, expressing either <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554004">yellow</a> or <a class="normal-link-page" href="http://parts.igem.org/Part:BBa_K1554005">blue</a> chromoprotein, and a male sterility module.</p><br/> |
<div align="center"><img src="https://static.igem.org/mediawiki/2014/e/e2/VUPV_biosafety_constr_omega_undercover.jpg" alt="omega_undercover2"></img></div><br/><br/><br/><br/> | <div align="center"><img src="https://static.igem.org/mediawiki/2014/e/e2/VUPV_biosafety_constr_omega_undercover.jpg" alt="omega_undercover2"></img></div><br/><br/><br/><br/> | ||
Revision as of 17:52, 15 October 2014
Achievements > Parts
Our team sent 7 BioBrick Parts to the Registry.
Four of them could be sent thanks to the collaboration from the NRP-UEA-Norwich team, who very kindly provided us with their MoFlippers. These were the parts coding sequences for AtrΔ11, HarFAR and EaDAcT and the TA29 promoter.
We also designed a vector that has de backbone of the Biobrick vector pSB1A3 but compatible with GoldenBraid 2.0. It contains two BsmBI restriction sites which leave the required overhangs to perform a GoldenBraid ligation of two transcriptional units in alpha vectors. We call it "omega undercover" as it works in a similar way of the GoldenBraid 2.0 omega vector but with a pSB1A3 backbone.
Using the omega undercover plasmid, we constructed the two biosafety devices which consist on an identity preservation module, expressing either yellow or blue chromoprotein, and a male sterility module.
<groupparts>iGEM014 Valencia_UPV</groupparts>
