Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/Jun

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<td width="100px"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/May"><img src="https://static.igem.org/mediawiki/2014/b/b9/Bielefeld-CeBiTec_2014-08-14_Arrrow-left.png" width="50px"> </a></td>
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<td style="padding:30px; width:400px"><h1> June </h1></td>
<td style="padding:30px; width:400px"><h1> June </h1></td>
<td width="100px" align="right"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/Jul"><img src="https://static.igem.org/mediawiki/2014/9/9b/Bielefeld-CeBiTec_2014-08-14_Arrow-right.png" width="50px"> </a>      </td>
<td width="100px" align="right"><a href="https://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Journal/rMFC/Jul"><img src="https://static.igem.org/mediawiki/2014/9/9b/Bielefeld-CeBiTec_2014-08-14_Arrow-right.png" width="50px"> </a>      </td>
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                <a href="#Week1">Week 1 &nbsp;&nbsp;&nbsp; 06/02 - 06/08</a>
 
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                <a  style="font-size:24px" href="#"><p style="margin-left:42%">Week 1 &nbsp;&nbsp;&nbsp; 06/02 - 06/08</p></a>
 
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                <a href="#Week2">Week 2 &nbsp;&nbsp;&nbsp; 06/09 - 06/15</a>
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                <a  style="font-size:24px" href="#"><p style="margin-left:42%">Week 2 &nbsp;&nbsp;&nbsp; 06/09 - 06/15</p></a>
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                 <a  style="font-size:24px" href="#"><p style="margin-left:42%">Week 3 &nbsp;&nbsp;&nbsp; 06/16 - 06/22</p></a>
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                 <h6><a  style="font-size:24px" href="#">Week 3 &nbsp;&nbsp;&nbsp; 06/16 - 06/22</a></h6>
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<ul>
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<li><b>Initial cultivation experiments with neutral red </b></li>
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<ul>
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<li> This week we wanted to test the properties of neutral red during a cultivation on a small scale </li>
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<ul>
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<li> Cultivation of the <i>E. coli</i> strain with porines (attempt 1) and wildtyp as control (attempt 2) in LB-medium with 100 µM neutral red </li>
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<li> pH was set up at 8.75 </li>
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<li> Results </li>
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<ul>
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<li> Attempt 1 turned red (pH = 7.495)</li>
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<li> Attempt 2 remained yellow </li>
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</ul>
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                 <a  style="font-size:24px" href="#"><p style="margin-left:42%">Week 4 &nbsp;&nbsp;&nbsp; 06/23 - 06/29</p></a>
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                 <h6><a  style="font-size:24px" href="#">Week 4 &nbsp;&nbsp;&nbsp; 06/23 - 06/29</a></h6>
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<li><b> Reduction and oxidation of neutral red </b>
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<ul>
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<li> This week we checked the properties of neutral red under oxidizing and reducing conditions by adding chemical agents </li>
 +
<ul>
 +
<li> Reducing agent: sodium dithionite </li>
 +
<ul>
 +
<li> Red solution got reduced and turned colorless </li>
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<li> Got oxidised by air again afterwards </li>
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</ul>
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</ul>
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<ul>
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<li> Oxidizing agent: hydrogen peroxide </li>
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<ul>
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<li> Red solution stayed red </li>
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<li> Yellow solution stayed yellow </li>
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</ul>
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<li> Conclusion: the color of the solution depends on the pH </li>
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</ul>
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                 <a  style="font-size:24px" href="#"><p style="margin-left:42%">Week 5 06/30 - 07/06</p></a>
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                 <h6><a  style="font-size:24px" href="#">Week 5 06/30 - 07/06</a></h6>
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             <div class="content" style="margin-right:10%; margin-left:10%">
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<ul>
 +
<li><b> Further cultivation experiments with neutral red </b></li>
 +
<ul> 
 +
<li> This week we cultivated <i>E. coli</i> in LB-medium with 100 µM neutral red which was reduced by different concentrations of sodium dithionite before </li>
 +
<ul>
 +
<li> Attempts and controls </li>
 +
<ul>
 +
<li> Negative controls:
 +
<ul>
 +
<li> 0 µl of sodium dithionite </li>
 +
<li> 100 µl of sodium dithionite </li>
 +
<li> 200 µl of sodium dithionite </li>
 +
<li> 500 µl of sodium dithionite </li>
 +
</ul>
 +
<li> Positive control:
 +
<ul>
 +
<li> 0 µl sodium dithionite with <i>E. coli</i> </li>
 +
</ul>
 +
<li> Attempts: </li>
 +
<ul>
 +
<li>100 µl sodium dithionite with <i>E. coli</i></li>
 +
<li> 200 µl sodium dithionite with <i>E. coli</i> </li>
 +
<li> 500 µl sodium dithionite with <i>E. coli</i> </li>
 +
</ul>
 +
</ul>
 +
<li> Results </li>
 +
<ul>
 +
<li> Negative controls: all controls got colored probably through oxidation by air </li>
 +
<li> Positive control: turned yellow and turbid probably because of alkaline conditions and high cell density </li>
 +
<li> Attempts: all attempts turned red but no turbidity is visible probably because sodium dithionite could be toxic to the cells </li>
 +
</ul>
 +
</ul>
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</ul>
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Latest revision as of 16:31, 15 October 2014


June

Week 3     06/16 - 06/22
Week 3     06/16 - 06/22
  • Initial cultivation experiments with neutral red
    • This week we wanted to test the properties of neutral red during a cultivation on a small scale
      • Cultivation of the E. coli strain with porines (attempt 1) and wildtyp as control (attempt 2) in LB-medium with 100 µM neutral red
      • pH was set up at 8.75
      • Results
        • Attempt 1 turned red (pH = 7.495)
        • Attempt 2 remained yellow
Week 4     06/23 - 06/29
Week 4     06/23 - 06/29
  • Reduction and oxidation of neutral red
    • This week we checked the properties of neutral red under oxidizing and reducing conditions by adding chemical agents
      • Reducing agent: sodium dithionite
        • Red solution got reduced and turned colorless
        • Got oxidised by air again afterwards
      • Oxidizing agent: hydrogen peroxide
        • Red solution stayed red
        • Yellow solution stayed yellow
      • Conclusion: the color of the solution depends on the pH
Week 5     06/30 - 07/06
Week 5 06/30 - 07/06
  • Further cultivation experiments with neutral red
    • This week we cultivated E. coli in LB-medium with 100 µM neutral red which was reduced by different concentrations of sodium dithionite before
      • Attempts and controls
        • Negative controls:
          • 0 µl of sodium dithionite
          • 100 µl of sodium dithionite
          • 200 µl of sodium dithionite
          • 500 µl of sodium dithionite
        • Positive control:
          • 0 µl sodium dithionite with E. coli
        • Attempts:
          • 100 µl sodium dithionite with E. coli
          • 200 µl sodium dithionite with E. coli
          • 500 µl sodium dithionite with E. coli
      • Results
        • Negative controls: all controls got colored probably through oxidation by air
        • Positive control: turned yellow and turbid probably because of alkaline conditions and high cell density
        • Attempts: all attempts turned red but no turbidity is visible probably because sodium dithionite could be toxic to the cells

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