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| - | {{:Team:Brasil-SP/Templates/Header}}
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| - | <html>
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| - | <!--main content -->
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| - | <table width="70%" align="center">
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| - | <p><h2>Measurement Interlab Study</h2></p>
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| - | <h1><h1>
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| - | </li>
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| - | <a href="#characterizationprotocols">Protocols</a>
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| - | <a href="#results">Results</a>
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| - | <!--track measurement-->
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| - | <p align="justify"> As part of the first international interlab measurement study in synthetic biology, all Measurement Track teams were required to obtain fluorescence data for three specific genetic devices. They are<p/>
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| - | <p align="center"> Biobrick device 1: BBa_I20260, an existing device </p>
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| - | <br>
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| - | <a href="https://static.igem.org/mediawiki/2014/c/c1/Assembly_Biobrick_Device_1_Measurement_Brasilsp.pdf">Assembly Protocol Biobrick Device 1</a>
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| - | <img src="https://static.igem.org/mediawiki/2014/d/d0/Device1_measurement_Brasil-SP.png" width="330" height="auto">
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| - | <p align="center"> Biobrick device 2: BBa_J23101 + BBa_E0240, a new device built by each team</p>
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| - | <a href="https://static.igem.org/mediawiki/2014/d/d3/Assembly_Biobrick_Devices_2_and_3_Measurement_Interlab_Study.pdf"> Assembly Protocol Biobrick Device 2 and 3 </a>
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| - | <img src="https://static.igem.org/mediawiki/2014/8/84/Device2_measurement_brasilsp.png" width="350" height="auto">
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| - | <p align="center"> Biobrick device 3: BBa_J23115 + BBa_E0240, a new device built by each team.<br><strong>Note:</strong> Compared to biobrick device 1 and 2, biobrick device 3 was constructed with a different promoter (BBa_J23115).</p>
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| - | <a href="https://static.igem.org/mediawiki/2014/d/d3/Assembly_Biobrick_Devices_2_and_3_Measurement_Interlab_Study.pdf"> Assembly Protocol Biobrick Device 2 and 3 </a>
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| - | <img src="https://static.igem.org/mediawiki/2014/9/93/Device3_measurement_brasilsp.png" width="350" height="auto">
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| - | <li><a href="https://static.igem.org/mediawiki/2014/4/40/Interlab_Study_Measurement_Flow_Cytometer_Brasil-SP_Team.pdf">Flow Cytometry Protocol-Samples Preparation</a></li>
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| - | <br><br>
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| - | <p align="justify"> After colect and store data, it was necessary to analyze our results. Data file format standard for flow cytometry is FCS (Flow Cytometry Standard), it contains all information about data acquired, instrument used and sample. In order to extract useful data we converted .fcs file to .csv using GenePattern Flow Cytometry suite.</p>
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| - | <!--end characterization protocols-->
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| - | <div id="results" class="row">
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| - | <h2 align="left"><b>Results</b></h2></ul>
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| - | <p align="justify"> For the proposed specific genetic devices, we have measured fluorescence by <strong>Flow Cytometry</strong> and <strong>Fluorometry</strong>. Device 1 and 2 are equivalent assemblies, however, we did not expect equal fluorescence values since device 1 was an existing device and 2 was built. When it comes to device 3, only the promoter was changed from BBa_J23101 to BBa_J23115, in all likelihood, differing in strength.</p>
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| - | <img src="https://static.igem.org/mediawiki/2014/d/d8/Fl1-h_interlabstudy_fcyt_brasilsp.png" width="500" height="auto">
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| - | <br><span style="font-size: 10-small">Flow cytometry - Mean values</span>
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| - | </html>
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| - | {{:Team:Brasil-SP/Templates/Footer}}
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