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| - | {{:Team:Brasil-SP/Templates/Header}}
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| - | <html>
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| - | <!--main content -->
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| - | <table width="70%" align="center">
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| - | <p><h2>Measurement Interlab Study</h2></p>
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| - | <h1><h1>
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| - | <a href="#characterizationprotocols">Protocols</a>
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| - | <a href="#results">Results</a>
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| - | <!--track measurement-->
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| - | <p align="justify"> As part of the first international interlab measurement study in synthetic biology, all Measurement Track teams were required to obtain fluorescence data for three specific genetic devices. They are<p/>
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| - | <p align="center"> Biobrick device 1: BBa_I20260, an existing device </p>
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| - | <a href="https://static.igem.org/mediawiki/2014/c/c1/Assembly_Biobrick_Device_1_Measurement_Brasilsp.pdf">Assembly Protocol Biobrick Device 1</a>
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| - | <img src="https://static.igem.org/mediawiki/2014/d/d0/Device1_measurement_Brasil-SP.png" width="330" height="auto">
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| - | <p align="center"> Biobrick device 2: BBa_J23101 + BBa_E0240, a new device built by each team</p>
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| - | <a href="https://static.igem.org/mediawiki/2014/d/d3/Assembly_Biobrick_Devices_2_and_3_Measurement_Interlab_Study.pdf"> Assembly Protocol Biobrick Device 2 and 3 </a>
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| - | <img src="https://static.igem.org/mediawiki/2014/8/84/Device2_measurement_brasilsp.png" width="350" height="auto">
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| - | <p align="center"> Biobrick device 3: BBa_J23115 + BBa_E0240, a new device built by each team.<br><strong>Note:</strong> Compared to biobrick device 1 and 2, biobrick device 3 was constructed with a different promoter (BBa_J23115).</p>
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| - | <a href="https://static.igem.org/mediawiki/2014/d/d3/Assembly_Biobrick_Devices_2_and_3_Measurement_Interlab_Study.pdf"> Assembly Protocol Biobrick Device 2 and 3 </a>
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| - | <img src="https://static.igem.org/mediawiki/2014/9/93/Device3_measurement_brasilsp.png" width="350" height="auto">
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| - | <li><a href="https://static.igem.org/mediawiki/2014/4/40/Interlab_Study_Measurement_Flow_Cytometer_Brasil-SP_Team.pdf">Flow Cytometry Protocol-Samples Preparation</a></li>
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| - | <!--end characterization protocols-->
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| - | <div id="results" class="row">
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| - | <h2 align="left"><b>Results</b></h2></ul>
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| - | <p align="justify"> For the proposed specific genetic devices, we have measured fluorescence by <strong>Flow Cytometry</strong> and <strong>Fluorometry</strong>. Device 1 and 2 are equivalent assemblies, however, we did not expect equal fluorescence values since device 1 was an existing device and 2 was built. When it comes to device 3, only the promoter was changed from BBa_J23101 to BBa_J23115, in all likelihood, differing in strength.</p>
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| - | <img src="https://static.igem.org/mediawiki/2014/d/d8/Fl1-h_interlabstudy_fcyt_brasilsp.png" width="500" height="auto">
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| - | <br><span style="font-size: 10-small">Flow cytometry - Mean values</span>
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| - | {{:Team:Brasil-SP/Templates/Footer}}
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