Team:Brasil-SP/Notebook/Interlab Study

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                              <h1><h1>
 
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<a href="#characterizationprotocols">Protocols</a>
 
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<p><h2>Measurement Interlab Study</h2></p>
 
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<p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;As part of the first international interlab measurement study in synthetic biology, all Measurement Track teams were required to obtain fluorescence data for three specific genetic devices. They are<p/>
 
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<p align="center"> Biobrick device 1: BBa_I20260, an existing device </p>
 
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<a href="https://static.igem.org/mediawiki/2014/c/c1/Assembly_Biobrick_Device_1_Measurement_Brasilsp.pdf">Assembly Protocol Biobrick Device 1</a>
 
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<p align="center"> Biobrick device 2: BBa_J23101 + BBa_E0240, a new device built by each team</p>
 
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<a href="https://static.igem.org/mediawiki/2014/d/d3/Assembly_Biobrick_Devices_2_and_3_Measurement_Interlab_Study.pdf"> Assembly Protocol Biobrick Device 2 and 3 </a>
 
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<p align="center"> Biobrick device 3: BBa_J23115 + BBa_E0240, a new device built by each team.<br><strong>Note:</strong> Compared to biobrick device 1 and 2, biobrick device 3 was constructed with a different promoter (BBa_J23115).</p>
 
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<a href="https://static.igem.org/mediawiki/2014/d/d3/Assembly_Biobrick_Devices_2_and_3_Measurement_Interlab_Study.pdf"> Assembly Protocol Biobrick Device 2 and 3 </a>
 
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    <li><a href="https://static.igem.org/mediawiki/2014/4/40/Interlab_Study_Measurement_Flow_Cytometer_Brasil-SP_Team.pdf">Flow Cytometry Protocol-Samples Preparation</a></li>
 
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<h2 align="left"><b>Results</b></h2></ul>
 
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<p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; For the proposed specific genetic devices, we have measured fluorescence by <strong>Flow Cytometry</strong> and <strong>Fluorometry</strong>. Device 1 and 2 are equivalent assemblies, however, we did not expect equal fluorescence values since device 1 was an existing device and 2 was built. When it comes to device 3, only the promoter was changed, so it would be possible to compare different </p>
 
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<br><span style="font-size: 10-small">Flow cytometry - Mean values</span>
 
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Latest revision as of 16:19, 15 October 2014