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Team:SUSTC-Shenzhen/Notebook/Parts building
From 2014.igem.org
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For the linear backbone were so few and the backbone of BBa_J04450 was pSB1C3, we decide to cut it by XbaI and PstI restriction enzyme and recycle the about 2000bp vector as the backbone for biobricks. Of course, one advantage to make it as backbone is that we can recognize whether the gene we designed have inserted. Because if not, the bacterium will express red chromoprotein which one we unnecessary to pick up. | For the linear backbone were so few and the backbone of BBa_J04450 was pSB1C3, we decide to cut it by XbaI and PstI restriction enzyme and recycle the about 2000bp vector as the backbone for biobricks. Of course, one advantage to make it as backbone is that we can recognize whether the gene we designed have inserted. Because if not, the bacterium will express red chromoprotein which one we unnecessary to pick up. | ||
| - | =week 4, Aug= | + | ==week 4, Aug== |
| - | ==2014/08/22== | + | ===2014/08/22=== |
<span style="color: red">''<u>Transfect the backbone of biobricks</u>'' | <span style="color: red">''<u>Transfect the backbone of biobricks</u>'' | ||
Revision as of 16:13, 15 October 2014
Parts building
design and building
Contents |
By Rifei Chen
For the linear backbone were so few and the backbone of BBa_J04450 was pSB1C3, we decide to cut it by XbaI and PstI restriction enzyme and recycle the about 2000bp vector as the backbone for biobricks. Of course, one advantage to make it as backbone is that we can recognize whether the gene we designed have inserted. Because if not, the bacterium will express red chromoprotein which one we unnecessary to pick up.
week 4, Aug
2014/08/22
Transfect the backbone of biobricks
