Attributions

Project Design and Wet Lab Experiments

The BananaGuard project consists of multiple wet lab modules. Jeremy constructed the fusaric acid sensor while Wen and Tjaša worked on the expression of fungal growth inhibitors. Tjaša focused on chitinase expression while Wen dedicated her time to the other three compounds. The fusaric acid resistance in the biological control agent was Rik’s project. The final application in the greenhouse was executed by Marlene.

Teresa and Kevin worked on the horizontal gene transfer inhibition and Max, Michiel and Marlene (M&M&M) worked on the kill-switch. Max was also responsible for in situ experiments and promoter design.

Walter was working in the wet lab to obtain production kinetic data for the genome-scale metabolic model of Pseudomonas putida.

The iGEM team Wageningen also participated in the Interlab study. The corresponding experiments were performed by Marlene and Rik.

Modeling

Models were done for the metabolic stress of the toxin production, the kill-switch and the horizontal gene transfer. The kill-switch model and system model under cell division were done by Bob and Walter who modeled the metabolic stress of the designed system.

Human Practice

The iGEM team Wageningen established contact between the National Institute for Public Health and the Environment (RIVM) and all Dutch iGEM teams. Bob was their main contact person and Jeremy organized the national meet up at which RIVM participated. For the collaboration with Synenergene an application scenarios was generated. Miguel, Max, Rik and Wen were involved in this task.

Wiki Construction

The Wiki was constructed by Rik, Teresa and Tjaša who learned how to design a wiki in one summer. Combining everyone’s skills we managed to construct an interactive and user friendly wiki, giving the right importance to the layout as well as to the content. Alba Ramos Álvarez, a Spanish Arts and Design student, and Wen supported the Wiki-team with their illustration skills. Also, Sophia, the team captain of XMU-China 2012 helped us a lot with the design and coding of the wiki. All icons used in this wiki page were made by Icons8 from www.flaticon.com licensed by CC BY 3.0.

Finances

The sponsoring team consisted of Kevin, Rik, Walter, Marlene and Wen. The whole sponsor team was involved in finding and contacting our sponsors. Rik and Kevin were in charge of regulating money flow while Wen and Marlene were responsible for managing the money for different purposes. Wen was in charge of ordering lab supplies and Marlene organized the trip to the Jamboree.

Safety

Responsible for safety within the lab and safety regulations by the government were Tjaša with Miguel's help. Marlene was in charge of providing all data for the GMO-permit extension which was needed for greenhouse experiment.

Other Team Roles

The Captain of BananaGuard was Wen supported by Teresa who was the secretary and Michiel who functioned as vice secretary. Marlene arranged our amazing team clothes.

Supervisors

Christian Fleck was the head of the whole team. Kees van der Ark, Marnix Vlot, Nico Claassens and Stamatios Damalas were the supervisors of all the subprojects involving experimental work in the laboratory, while Robert Smith and Ruben van Heck supervised the modelling part. Gert Kema and Fernando Garcia Bastidas from Plant Research International supervised the greenhouse and Fusarium oxysporum experiments.