Team:UESTC-China/Design
From 2014.igem.org
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The ribulose monophosphate (RuMP) pathway is one of the HCHO-fixation pathways found in microorganisms called methylotrophs, which utilize one-carbon compounds as the sole carbon source. The key enzymes of this pathway are 3-hexulose-6-phosphate synthase (HPS), which fixes HCHO to D-ribulose-5-phosphate (Ru5P) to produce D-arabino-3-hexulose 6-phosphate (Hu6P), and 6-phospho-3- hexuloisomerase (PHI), which converts Hu6P to fructose 6-phosphate (F6P).The two key enzymes work in chloroplast both.We will use fusion expression to conduct heterologous expression in tobacco( Li-mei Chen et al,2010). Here are some datas from the paper. | The ribulose monophosphate (RuMP) pathway is one of the HCHO-fixation pathways found in microorganisms called methylotrophs, which utilize one-carbon compounds as the sole carbon source. The key enzymes of this pathway are 3-hexulose-6-phosphate synthase (HPS), which fixes HCHO to D-ribulose-5-phosphate (Ru5P) to produce D-arabino-3-hexulose 6-phosphate (Hu6P), and 6-phospho-3- hexuloisomerase (PHI), which converts Hu6P to fructose 6-phosphate (F6P).The two key enzymes work in chloroplast both.We will use fusion expression to conduct heterologous expression in tobacco( Li-mei Chen et al,2010). Here are some datas from the paper. | ||
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- | <div align="center"><img style="width: | + | <div align="center"><img style="width:50% ;" src="https://static.igem.org/mediawiki/2014/a/a8/Figuestc.jpg" naptha_cursor="text"> |
<p style="position:relative; left:20px; padding:19 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:700px; color:#1b1b1b;"> | <p style="position:relative; left:20px; padding:19 5px; font-size:20px; font-family: calibri, arial, helvetica, sans-serif; font-style: calibri; text-align:justify; width:700px; color:#1b1b1b;"> | ||
<strong>Fig.1</strong> | <strong>Fig.1</strong> |
Revision as of 12:46, 15 October 2014