1Main functions

1.1Submit a Crisper-X

requestURL: getMain.php

1.2Get result

Get the result given the request id.

URL	getResult.php
POST	id
Return	Result in json style,with status=0, 1, 2 means ok, error and not finished respectively.
Example	POST: id=355Return: {“status”:2,“message”:“not finished yet”}

1.3Get requests History

Get requests history. Unregistered users will get the global history.

URL	getHistory.php
POST	token
Return	Top ten records, ordered by time, in Json array style
Example	POST: token=005536bf21179e54370c75b6fb136f2aReturn: [{“request id”:“351”,“status”:“0”},{“request id”:“355”,“status”:“2”}]
See also	Login [ section 2.1 ]

2User Management

2.1Login

Login(Sign in) using Username and Password. A token will be returned forfuture authentication.

URL	login/
POST	name(username), pswd(password)
Return	32 byte token when login succeed, or ’-’ otherwise.
Example	POST: name=guest&pswd=1234Return: 005536bf21179e54370c75b6fb136f2a

2.2Log out

Delete user log information and Log out.

URL	login/logout.php
POST	token
Return	Nothing.
See also	Login [ section 2.1 ]

2.3Sign Up

Create a new account with username, password and email.

URL	login/signup.php
POST	name(username, unique),pswd(password, encrypt using md5),email(unique).
Return	”Sign In Succeed” or error information.

2.4Update user’s Information

Update your password and/or email address.

URL	login/signup.php
POST	name(username),pswd(old password),newpswd(new password *),newemail(new email *),* Not necessary
Return	’+’: ok, ’-’: failed.

3File/Specie Upload

3.1Check upload

Check if this token can upload files

URL	upload/check.php
POST	status=0(Yes)/1(No) and message if status=1 in JSON.
Return	’+’: ok, ’-’: failed.
Example	{”status”:1, ”message”:”Authentication failed”}

3.2Upload file

Upload your file.

URL	upload/
POST	token, filename(no longer than 255),note(no longer than 1000), file
Return	A(Upload succeed) or N(Something goes wrong)

3.3Import

Import data from file(s).

URL	upload/import.php
POST	token, command(in json, see section3.7)
Return	New specie Name(start with number), or ’N’ for ’Something goes wrong’

3.4Viewmyfiles

View all my files.

URL	upload/viewmyfiles.php
POST	token
Return	All my files in JSON Array Style
Example	[{”fileName”:”NC 001133-chromosome1”,”note”:”Saccharomycetes’s chromosome”}]

3.5Viewmyspecies

View all my species.

URL	upload/viewmyspecies.php
POST	token
Return	All my species in JSON Array Style
Example	[ {”specie”:”Saccharomyces-cerevisiae”, ”PAMs”:[{”PAM”:”NGG”},{”PAM”:”NRG”}], ”chromosomes”:[{”chromosome”:”NC 001147-chromosome1”}, {”chromosome”:”NC 001147-chromosome16”} ]

3.6Deletemyfiles

Delete your file.

URL	upload/deletemyfiles.php
POST	token, filename
Return	A(Delete succeed) or N(Something goes wrong)

3.7json.command.txt

How to write commands in Import(section 3.3) method.

The structure of a command:

		{
		specie 			varchar(96) 		Name of the new Specie.
		files 				array 			All correlative chromosome files(.fna).
		[
			fileName varchar(255) File name
		]
		PAM 			varchar(20)			PAM
		}

Example:

{”specie”:”testSpecie”,”files”:[{”fileName”:”NC 012971”}],”PAM”:”NGG”}

4josn.txt

4.1Input

		type 1 1=Knockout
		specie E.coil Specie
		gene thrA Gene Name
		location Chr2:336..2798 Location
		pam NGG PAM Sequence [1]
		r1 0.65 r1, r2=1-r1
		length 20 nt length
		region ”0000” Region filter. 1 for in and 0 for out.
		EXON,INTRON,UTR,INTERGENIC
		rfc ”000000” 1 for use and 0 for not.
		RFC: 10, 12, 12a, 21, 23, 25

4.2Input Example

		{
		“specie”	:	“Saccharomyces-cerevisiae”,
		“location”	:	“NC 001144-chromosome12:1..500”,
		“pam”		:	“NGG”,
		“rfc”		:	“100101”
		}

4.3Output

		status 		0 				Request Status. (0: ok, 1: failed, 2: still running)
		message  	no args 			Return message
		specie  		E.coil 			Specie
		gene  		thrA 				Gene name
		location  		Chr2:336..2798 	Location
		region  		array[]			Selected sequence information
		{
		endpoint		1807			Region end point
		description		intergenic		Region information
		}
		result array[] Top 50 Only
		{
		grna TC. . . CGG(20) sgRNA sequence
		position Chr2:15413205 Location
		strand + Which DNA chain (+/-)
		region exon Region of this sgRNA
		total score 86 Sguide
		Sspe 93 Sspe
		Seff 7 Seff
		count 2 Nmm
		offtarget array[] Top 20 Only
		{
		osequence CT. . . TGGG(20) possible-offtarget sgRNA sequence
		oscore 3.5 Smm
		omms 4 Nmm
		oposition Chr3:4158 Location of po-sgRNA
		ostrand + Which DNA chain (+/-)
		oregion Intergenic Region of this sgRNA
		}
		}

4.4Output example when ok:

		{
		”status”: 0,
		”message”: {
		”specie”: ”E.coli”,
		”kind”: ”E.coli K12-MG1655”,
		”gene”: ””,
		”location”: ”1:336..2798”
		},
		”result”: [{”key”: ”#1”,
		”grna”: ”GAAGTTCGGCGGTACATCAGTGG”,
		”position”: ”1:368”,
		”total score”: 100,
		”count”: 0,
		”offtarget”: []
		}, {
		”key”: ”#2”,
		”grna”: ”TAATGAAAAAGGCGAACTGGTGG”,
		”position”: ”1:935”,
		”total score”: 100,
		”count”: 0,
		”offtarget”: []
		}, {
		”key”: ”#3”,
		”grna”: ”TGGAAAGCAATGCCAGGCAGGGG”,
		”position”: ”1:427”,
		”total score”: 100,
		”count”: 0,
		”offtarget”: []
		}, {
		”key”: ”#4”,
		”grna”: ”CAAAATCACCAACCACCTGGTGG”,
		”position”: ”1:479”,
		”total score”: 100,
		”count”: 0,
		”offtarget”: []
		},{
		”key”: ”#44”,
		”grna”: ”ATTTTTGCCGAACTTTTGACGGG”,
		”position”: ”1:564”,
		”total score”: 91,
		”count”: 2,
		”offtarget”: [{
		”osequence”: ”ATTTTCGCCAAACATTTGGCAGG”,
		”oscore”: 0.954750,
		”omms”: 4,
		”ostrand”: ”-”,
		”oposition”: ”1:1924344”,
		”oregion”: ”exco”
		}, {
		”osequence”: ”ATTGTTGCGCAACTTTTGGCTGG”,
		”oscore”: 0.480417,
		”omms”: 4,
		”ostrand”: ”-”,
		”oposition”: ”1:3827949”,
		”oregion”: ”exco”
		}]
		}]
		}

4.5 Output example when failed:

		{
		“status”: 1,
		“message”: “illegal args”
		}

4.6 Appendix. Equations

R=A,G; M=A,C; W=A,T; S=C,G; K=G,T; Y=C,T; H=A,C,T; V=A,C,G; B=C,G,T; D=A,G,T; N=A, G, C, T