Team:Brasil-SP/Notebook/Interlab Study

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<p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; For the proposed specific genetic devices, we have measured fluorescence by <strong>Flow Cytometry</strong> and <strong>Fluorometry</strong>. Device 1 and 2 are equivalent assemblies, however, we did not expect equal fluorescence values since device 1 was an existing device and 2 was built. When it comes to device 3, only the promoter was changed, so it would be possible to compare different </p>
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<p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; For the proposed specific genetic devices, we have measured fluorescence by <strong>Flow Cytometry</strong> and <strong>Fluorometry</strong>. Device 1 and 2 are equivalent assemblies, however, we did not expect equal fluorescence values since device 1 was an existing device and 2 was built. When it comes to device 3, only the promoter was changed.  </p>

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Measurement Interlab Study

     As part of the first international interlab measurement study in synthetic biology, all Measurement Track teams were required to obtain fluorescence data for three specific genetic devices. They are

Biobrick device 1: BBa_I20260, an existing device


Assembly Protocol Biobrick Device 1

Biobrick device 2: BBa_J23101 + BBa_E0240, a new device built by each team


Assembly Protocol Biobrick Device 2 and 3


Biobrick device 3: BBa_J23115 + BBa_E0240, a new device built by each team.
Note: Compared to biobrick device 1 and 2, biobrick device 3 was constructed with a different promoter (BBa_J23115).


Assembly Protocol Biobrick Device 2 and 3

    Results

      For the proposed specific genetic devices, we have measured fluorescence by Flow Cytometry and Fluorometry. Device 1 and 2 are equivalent assemblies, however, we did not expect equal fluorescence values since device 1 was an existing device and 2 was built. When it comes to device 3, only the promoter was changed.


Flow cytometry - Mean values