Team:Brasil-SP/Notebook/Interlab Study

(Difference between revisions)

Revision as of 02:45, 15 October 2014

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Protocols Results Measurement Interlab Study As part of the first international interlab measurement study in synthetic biology, all Measurement Track teams were required to obtain fluorescence data for three specific genetic devices. They are
Biobrick device 1: BBa_I20260, an existing device Assembly Protocol Biobrick Device 1
Biobrick device 2: BBa_J23101 + BBa_E0240, a new device built by each team Assembly Protocol Biobrick Device 2 and 3
Biobrick device 3: BBa_J23115 + BBa_E0240, a new device built by each team. Note: Compared to biobrick device 1 and 2, biobrick device 3 was constructed with a different promoter (BBa_J23115). Assembly Protocol Biobrick Device 2 and 3
Flow Cytometry Protocol-Samples Preparation
Results For the proposed specific genetic devices, we have measured fluorescence by Flow Cytometry and Fluorometry. Device 1 and 2 are equivalent assemblies, however, we did not expect equal fluorescence values since device 1 was an existing device and 2 was built by each team. Flow cytometry - Mean values

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-<p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; For the specific genetic devices proposed, we have measured fluorescence by <strong>Flow Cytometry</strong> and <strong>Fluorometry</strong>.</p>
+<p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; For the proposed specific genetic devices, we have measured fluorescence by <strong>Flow Cytometry</strong> and <strong>Fluorometry</strong>. Device 1 and 2 are equivalent assemblies, however, we did not expect equal fluorescence values since device 1 was an existing device and 2 was built by each team.</p>
 <img src="https://static.igem.org/mediawiki/2014/d/d8/Fl1-h_interlabstudy_fcyt_brasilsp.png" width="500" height="auto">
-<br><span style="font-size: 10-small">Flow cytometry</span>
+<br><span style="font-size: 10-small">Flow cytometry - Mean values</span>