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Template:Team:USyd-Australia/Calendar/Events List
From 2014.igem.org
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| - | { title: | + | { title: "Plasmid Prep psB6A1, Finish", |
| - | start: new Date(2014, | + | start: new Date(2014,6,09), |
| - | description: | + | description: "Result: Plasmid prep yielded 4x100uL tubes<br><br>- Rokiah"}, |
| - | { title: | + | { title: "Plasmid prep of pUS44, Checking: Setup restriction digest and incubate", |
| - | start: new Date(2014, | + | start: new Date(2014,6,09), |
| - | description: | + | description: "Details: restriction digest to check plasmids (EcoR1, Ssp1, null). Incubated at 37C overnight.<br><br>- Abi"}, |
| - | { title: | + | { title: "Plasmid prep of pUS44, Checking: finish digests, gel electrophoresis of digests", |
| - | start: new Date(2014, | + | start: new Date(2014,6,10), |
| - | description: | + | description: "Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. <br><br>- Abi"}, |
| - | { title: | + | { title: "Plasmid prep of psB6A1, Checking: finish digests, gel electrophoresis of digests", |
| - | start: new Date(2014, | + | start: new Date(2014,6,10), |
| - | description: | + | description: "Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. pSB6A1 yielded 5104.1ng/uL DNA & (A260/280) 1.62.<br><br>Conclusion: Possible protein contamination to samples<br><br>- Abi"}, |
| - | { title: | + | { title: "Plasmid prep of pSB6A1, Re-doing digest", |
| - | start: new Date(2014, | + | start: new Date(2014,6,11), |
| - | description: | + | description: "Details: Restriction digest to check plasmids (EcoR1, EcoR1+Pst1, EcoR1+Nde1). <br><br>- Jeanne"}, |
| - | { title: | + | { title: "Plasmid prep of pUS44, Re-doing digest", |
| - | start: new Date(2014, | + | start: new Date(2014,6,15), |
| - | description: | + | description: "Details: Restriction digest to check plasmids (EcoR1, EcoR1+Pst1, EcoR1+Nde1). <br><br>- Abi"}, |
| - | { title: | + | { title: "Plasmid prep of pUS44, Re-doing digest", |
| - | start: new Date(2014, | + | start: new Date(2014,6,17), |
| - | description: | + | description: "Details: Restriction digest. Did the protocol change?<br><br>Conclusion: Nanospec: 395.5ng/mL DNA yield<br><br>- Abi & Tom"}, |
| - | { title: | + | { title: "Construction of pUS201, PCR of pSB6A1 using iGEM401/402 primers ", |
| - | start: new Date(2014, | + | start: new Date(2014,6,25), |
| - | description: | + | description: "Details: PCR Master mix with 1401/1402 primers. Used iGEM pSB6A1. Ran on regular program PCR ~2HRS<br><br>Result: Yield 1018.6ng/uL and low 260/280<br><br>Conclusion: ?<br><br>- Callum"}, |
| - | { title: | + | { title: "Construction of pUS201, Checking: finish pcr, gel electrophoresis of digests", |
| - | start: new Date(2014, | + | start: new Date(2014,6,25), |
| - | description: | + | description: "Details: Gel electrophoresis (100V, 400mA, 1hr). Stained in gel red for 30min<br><br>Result: Expected fragment size of 2.6kB from pSB6A1<br><br>Conclusion: Primers amplified expected product from pSB6A1<br><br>- Callum"}, |
| - | { title: | + | { title: "Construction of pUS204, Restriction digest of pSB1C3", |
| - | start: new Date(2014, | + | start: new Date(2014,6,30), |
| - | description: | + | description: "Details: Restriction digest (EcoR1+Pst1)<br><br>Result: Yield 25ng/mL<br><br>Conclusion: Enough to use for gibson assembly<br><br>- Callum"}, |
| - | { title: | + | { title: "Construction of pUS201, PCR of pSB1C3 using iGEM403/1404 primers ", |
| - | start: new Date(2014, | + | start: new Date(2014,6,31), |
| - | description: | + | description: "Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Yield 329.4ng/uL, low 260/280<br><br>- Callum"}, |
| - | { title: | + | { title: "Construction of pUS204, Transformation: pUS204 into top10 cells", |
| - | start: new Date(2014, | + | start: new Date(2014,6,31), |
| - | description: | + | description: "Details: Transformation (competent top10, pUS204). Incubated on AmpR plates for 2hrs<br><br>Result: Some AmpR colonies present, no blue colonies detected<br><br>Conclusion: pUS024 did not successfully transform, \"far unlikely positive control worked\" <br><br>- ?"}, |
| - | { title: | + | { title: "construction of pUS201, see notebook: second GA and transformation successful???", |
| - | start: new Date(2014, | + | start: new Date(2014,7,01), |
| - | description: | + | description: ""}, |
| - | { title: | + | { title: "construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404", |
| - | start: new Date(2014, | + | start: new Date(2014,7,04), |
| - | description: | + | description: "Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>- Jeanne & Callum"}, |
| - | { title: | + | { title: "construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ", |
| - | start: new Date(2014, | + | start: new Date(2014,7,05), |
| - | description: | + | description: "Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>- Jeanne & Callum"}, |
| - | { title: | + | { title: "Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies", |
| - | start: new Date(2014, | + | start: new Date(2014,7,05), |
| - | description: | + | description: "Details: Incubated overnight to finish later<br><br>Result: Yield 5.7ng/ul, 1.61 260/280<br><br>Conclusion: Yield too low to use<br><br>- Jeanne & Callum"}, |
| - | { title: | + | { title: "Checking IntI1 from SamR\'s construct, Restriction digest: SamR\'s pSB1C3+ARApBAD", |
| - | start: new Date(2014,7, | + | start: new Date(2014,7,08), |
| - | description: | + | description: "Details: Restriction digest (EcoR1+Pst1), incubated at 37 C for 1hr<br><br>Result: Yield: 4.46 ug/uL. Stored in -20 C fridge<br><br>Conclusion: Yield much higher than estimate provided by SamR (50ng/uL). Useable<br><br>- Rokiah"}, |
| - | { title: | + | { title: "Checking pUS201 construct, Checking pUS201 with digests", |
| - | start: new Date(2014, | + | start: new Date(2014,7,12), |
| - | description: | + | description: "Details: Restriction Digest (uncut, EcoR1, HindIII, HindIII+BamHI)<br><br>Result: gel????? Results???<br><br>- ?"}, |
| + | { title: "Checking IntI1 from SamR\'s construct, Checking: SamR\'s digests on gel electrohoresis", | ||
| + | start: new Date(2014,7,12), | ||
| + | description: "Details: Gel electrophoresis (Spe1, uncut) at 120V for 1hr. Stained in gel red for ~30min.<br><br>Result: [12.08 SamR gel]. Cut lanes travelled further than uncut, as expected.<br><br>Conclusion: NOTE:: notes at the end cannot be read<br><br>- Rokiah"}, | ||
| + | { title: "Making IntI1 for pUS203, Restriction Digest: extracting SamR\'s AraC-pBAD from pSB1C3", | ||
| + | start: new Date(2014,7,13), | ||
| + | description: "Details: pSB1C3 with AraC-pBAD was incubated with *specific enzyme* at 80 C for 20 min. The enzyme was heat-killed<br><br>- andy"}, | ||
| + | { title: "Construction of pUS204, Restrction digestion of aeBlue gBlock", | ||
| + | start: new Date(2014,7,14), | ||
| + | description: "Details: Restriction Digestion (EcoR1+Pst1)<br><br>Result: Yield: 1.6ng/uL<br><br>Conclusion: Enough yield to continue with ligation<br><br>- abi and tom"}, | ||
| + | { title: "Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies", | ||
| + | start: new Date(2014,7,21), | ||
| + | description: "Details: Plasmid prep<br><br>Result: Yield: 3680.1ng/uL. 2.03 A260/280<br><br>Conclusion: A260/280 high ratio, may have contamination<br><br>- Callum"}, | ||
| + | { title: "Construction of pUS204, Ligation of pSB1C3 and aeBlue gBlock digestion products", | ||
| + | start: new Date(2014,7,28), | ||
| + | description: "Details: Ligation using T4 ligase and products. Incubated for 1 hr at room temperature and them left overnight in 4C. Enzymes heat-killed at 65C.<br><br>- Tom"}, | ||
| + | { title: "Construction of pUS204, Transformaiton of ligated products into Top10 cells", | ||
| + | start: new Date(2014,8,01), | ||
| + | description: "Details: Transformation and then incubated overnight at 37 C<br><br>- Abi and Tom"}, | ||
| + | { title: "Checking pUS201 construct, Re-checking the pUS201 by restriction digestion", | ||
| + | start: new Date(2014,8,04), | ||
| + | description: "Details: Restriction digestion (EcoR1, NdeI, NdeI/BamHI, NdeI/HindIII)<br><br>"}, | ||
| + | { title: "construction of PUS204, Junction PCR to check whether pUS204 has been ligated successfully", | ||
| + | start: new Date(2014,8,09), | ||
| + | description: "Details: Junction PCR with iGEM1407/iGEM1408 primers and Taq<br><br>Result: Products plated for…why?<br><br>- Tom"}, | ||
| + | { title: "Construction of pUS203, Checking: heat-killing and running a gel of products", | ||
| + | start: new Date(2014,8,10), | ||
| + | description: "Details: Heat killed construct at 80 C for 20 min. Ran a gel electrophoresis of products wfor 100V for 1hr.<br><br>Result: Gel failed.<br><br>Conclusion: Gibson Assembly attempted anyway, gel fail was not indication of no construct<br><br>- Tom"}, | ||
| + | { title: "Construction of pUS201, Plating out Pus201 Gibson Assembly", | ||
| + | start: new Date(2014,8,10), | ||
| + | description: "Details: Plated out GA products onto Amp plates.<br><br>- Tom"}, | ||
| + | { title: "Construction of pUS204, Junction PCR gel", | ||
| + | start: new Date(2014,8,10), | ||
| + | description: "Details: Gel electrophoresis with PCR products (overnight pSB1C3/aeBlue cultures, 1hr growth cultures, no plasmid colony, no template control)<br><br>Result: ??<br><br>Conclusion: ??<br><br>"}, | ||
| + | { title: "Checkin pUS203, Patch plate Int1I-2 and SamR construct", | ||
| + | start: new Date(2014,8,11), | ||
| + | description: "Details: GA product cultures transferred to patch plates using 25x \"small\" colonies and 25x\"large\" colonies.<br><br>Result: Colonies to be screened with junction PCR. Why patch plate?<br><br>Conclusion: ?<br><br>- ?"}, | ||
| + | { title: "Checking Pus203, Preperation of colony PCR of PUS203: of the \"small\" and \"large\" colonies on patch plate", | ||
| + | start: new Date(2014,8,12), | ||
| + | description: "Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>"}, | ||
| + | { title: "Checking Pus203, colony PCR of PUS203: of the \"small\" and \"large\" colonies on patch plate", | ||
| + | start: new Date(2014,8,15), | ||
| + | description: "Details: PCR with Hs463a and Hs464 primers and Taq.<br><br>Result: Expected fragment size was 473bp. No data received. ????<br><br>Conclusion: ?? Re-do?<br><br>- rokiah"}, | ||
] | ] | ||
}); | }); | ||
Latest revision as of 02:33, 15 October 2014
