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Team:Tuebingen/Notebook/Protocols/3A assembly
From 2014.igem.org
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<h3>Three part assembly</h3> | <h3>Three part assembly</h3> | ||
| + | |||
| + | <h2>Digestion</h2> | ||
| + | |||
| + | <h4>Reagents</h4> | ||
| + | <table border="0"> | ||
| + | <colgroup> | ||
| + | <col width="100"> | ||
| + | <col width="300"> | ||
| + | </colgroup> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">242 g</td> | ||
| + | <td>Tris</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">57.1 mL</td> | ||
| + | <td>pure acetic acid</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">0.5 M</td> | ||
| + | <td>EDTA 0.5 M pH 8.0</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <h4>Procedure</h4> | ||
| + | <p>Mix all the reagents and add to 1000 ml with distillated water. Adjust to pH 8.3. Store at room temperature.</p> | ||
Revision as of 23:18, 14 October 2014
Three part assembly
Digestion
Reagents
| 242 g | Tris |
| 57.1 mL | pure acetic acid |
| 0.5 M | EDTA 0.5 M pH 8.0 |
Procedure
Mix all the reagents and add to 1000 ml with distillated water. Adjust to pH 8.3. Store at room temperature.
