Team:Tuebingen/Notebook/Protocols/restriction

From 2014.igem.org

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<h3>Preparative restriction digests</h3>
<h3>Preparative restriction digests</h3>
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<h4>Reagents</h4>
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<table border="0">
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  <colgroup>
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    <col width="100">
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    <col width="300">
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  </colgroup>
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  <tr>
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    <td style="text-align: center">min. 2 µg</td>
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    <td>Plasmid DNA</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">2 µL</td>
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    <td>of each restriction enzyme</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">10 µL</td>
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    <td>10x NE buffer 2</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">1 µL</td>
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    <td>100x BSA</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">to 100 µL</td>
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    <td>H2O</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">0.5 µL</td>
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    <td>Pfu Polymerase (1 U/µL)</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">to 50 µL</td>
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    <td>H20</td>
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  </tr>
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</table>
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<h4>Procedure</h4>

Revision as of 23:02, 14 October 2014


Preparative restriction digests

Reagents

min. 2 µg Plasmid DNA
2 µL of each restriction enzyme
10 µL 10x NE buffer 2
1 µL 100x BSA
to 100 µL H2O
0.5 µL Pfu Polymerase (1 U/µL)
to 50 µL H20

Procedure

Retrieved from "http://2014.igem.org/Team:Tuebingen/Notebook/Protocols/restriction"