Team:Tuebingen/Notebook/Protocols/LB plates

From 2014.igem.org

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<h3>LB plates</h3>
<h3>LB plates</h3>
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<h4>Reagents</h4>
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<table border="0">
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  <colgroup>
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    <col width="100">
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    <col width="300">
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  </colgroup>
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  <tr>
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    <td style="text-align: center">16 g</td>
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    <td>Agar</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">1000 mL</td>
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    <td><a href="https://2014.igem.org/Team:Tuebingen/Notebook/Protocols/LB_medium">LB medium</a></td>
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  </tr>
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  <tr>
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    <td style="text-align: center">&nbsp;</td>
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    <td>Antibiotics</td>
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  </tr>
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</table>
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<h4>Procedure</h4>
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<p>Add agar to LB medium and heat in microwave until the agar has completely dissolved. Perform
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next step at laminar flow cabinet! Let cool down a bit. If required, add antibiotics to Agar-LB
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medium. Cast plates in petri dishes (approximately 20 ml per plate) and store them at 4 °C.</p>
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</div>
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</div>

Revision as of 22:12, 14 October 2014


LB plates

Reagents

16 g Agar
1000 mL LB medium
  Antibiotics

Procedure

Add agar to LB medium and heat in microwave until the agar has completely dissolved. Perform next step at laminar flow cabinet! Let cool down a bit. If required, add antibiotics to Agar-LB medium. Cast plates in petri dishes (approximately 20 ml per plate) and store them at 4 °C.

Retrieved from "http://2014.igem.org/Team:Tuebingen/Notebook/Protocols/LB_plates"